<i>CCN3</i>,<i> POSTN</i>, and <i>PTHLH</i> as potential key regulators of genomic integrity and cellular survival in iPSCs
<p dir="ltr">Reprogramming human somatic cells into a pluripotent state, achieved through the activation of well-defined transcriptional factors known as OSKM factors, offers significant potential for regenerative medicine. While OSKM factors are a robust reprogramming method, effici...
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2024
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| _version_ | 1864513509827543040 |
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| author | Nuha T. Swaidan (18595219) |
| author2 | Nada H. Soliman (18595222) Ahmed T. Aboughalia (18595225) Toqa Darwish (13222230) Ruba O. Almeshal (19256332) Azhar A. Al-Khulaifi (19256335) Rowaida Z. Taha (8854754) Rania Alanany (19256338) Ahmed Y. Hussein (19256341) Salam Salloum-Asfar (656363) Sara A. Abdulla (13902015) Abdallah M. Abdallah (8277564) Mohamed M. Emara (9913215) |
| author2_role | author author author author author author author author author author author author |
| author_facet | Nuha T. Swaidan (18595219) Nada H. Soliman (18595222) Ahmed T. Aboughalia (18595225) Toqa Darwish (13222230) Ruba O. Almeshal (19256332) Azhar A. Al-Khulaifi (19256335) Rowaida Z. Taha (8854754) Rania Alanany (19256338) Ahmed Y. Hussein (19256341) Salam Salloum-Asfar (656363) Sara A. Abdulla (13902015) Abdallah M. Abdallah (8277564) Mohamed M. Emara (9913215) |
| author_role | author |
| dc.creator.none.fl_str_mv | Nuha T. Swaidan (18595219) Nada H. Soliman (18595222) Ahmed T. Aboughalia (18595225) Toqa Darwish (13222230) Ruba O. Almeshal (19256332) Azhar A. Al-Khulaifi (19256335) Rowaida Z. Taha (8854754) Rania Alanany (19256338) Ahmed Y. Hussein (19256341) Salam Salloum-Asfar (656363) Sara A. Abdulla (13902015) Abdallah M. Abdallah (8277564) Mohamed M. Emara (9913215) |
| dc.date.none.fl_str_mv | 2024-02-05T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fmolb.2024.1342011 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/_i_CCN3_i_i_POSTN_i_and_i_PTHLH_i_as_potential_key_regulators_of_genomic_integrity_and_cellular_survival_in_iPSCs/26403925 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Biomedical and clinical sciences Clinical sciences iPSCs ESCs transcription factors genomic integrity cellular survival |
| dc.title.none.fl_str_mv | <i>CCN3</i>,<i> POSTN</i>, and <i>PTHLH</i> as potential key regulators of genomic integrity and cellular survival in iPSCs |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Reprogramming human somatic cells into a pluripotent state, achieved through the activation of well-defined transcriptional factors known as OSKM factors, offers significant potential for regenerative medicine. While OSKM factors are a robust reprogramming method, efficiency remains a challenge, with only a fraction of cells undergoing successful reprogramming. To address this, we explored genes related to genomic integrity and cellular survival, focusing on iPSCs (A53T-PD1) that displayed enhanced colony stability. Our investigation had revealed three candidate genes <i>CCN3, POSTN, </i>and <i>PTHLH</i> that exhibited differential expression levels and potential roles in iPSC stability. Subsequent analyses identified various protein interactions for these candidate genes. <i>POSTN</i>, significantly upregulated in A53T-PD1 iPSC line, showed interactions with extracellular matrix components and potential involvement in Wnt signaling. CCN3, also highly upregulated, demonstrated interactions with TP53, CDKN1A, and factors related to apoptosis and proliferation. <i>PTHLH</i>, while upregulated, exhibited interactions with CDK2 and genes involved in cell cycle regulation. RT-qPCR validation confirmed elevated <i>CCN3</i> and<i> PTHLH</i> expression in A53T-PD1 iPSCs, aligning with RNA-seq findings. These genes’ roles in preserving pluripotency and cellular stability require further exploration. In conclusion, we identified <i>CCN3, POSTN, </i>and <i>PTHLH</i> as potential contributors to genomic integrity and pluripotency maintenance in iPSCs. Their roles in DNA repair, apoptosis evasion, and signaling pathways could offer valuable insights for enhancing reprogramming efficiency and sustaining pluripotency. Further investigations are essential to unravel the mechanisms underlying their actions.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Molecular Biosciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fmolb.2024.1342011" target="_blank">https://dx.doi.org/10.3389/fmolb.2024.1342011</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_fd844af30149ee18bb99192e47ca0729 |
| identifier_str_mv | 10.3389/fmolb.2024.1342011 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26403925 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | <i>CCN3</i>,<i> POSTN</i>, and <i>PTHLH</i> as potential key regulators of genomic integrity and cellular survival in iPSCsNuha T. Swaidan (18595219)Nada H. Soliman (18595222)Ahmed T. Aboughalia (18595225)Toqa Darwish (13222230)Ruba O. Almeshal (19256332)Azhar A. Al-Khulaifi (19256335)Rowaida Z. Taha (8854754)Rania Alanany (19256338)Ahmed Y. Hussein (19256341)Salam Salloum-Asfar (656363)Sara A. Abdulla (13902015)Abdallah M. Abdallah (8277564)Mohamed M. Emara (9913215)Biological sciencesGeneticsBiomedical and clinical sciencesClinical sciencesiPSCsESCstranscription factorsgenomic integritycellular survival<p dir="ltr">Reprogramming human somatic cells into a pluripotent state, achieved through the activation of well-defined transcriptional factors known as OSKM factors, offers significant potential for regenerative medicine. While OSKM factors are a robust reprogramming method, efficiency remains a challenge, with only a fraction of cells undergoing successful reprogramming. To address this, we explored genes related to genomic integrity and cellular survival, focusing on iPSCs (A53T-PD1) that displayed enhanced colony stability. Our investigation had revealed three candidate genes <i>CCN3, POSTN, </i>and <i>PTHLH</i> that exhibited differential expression levels and potential roles in iPSC stability. Subsequent analyses identified various protein interactions for these candidate genes. <i>POSTN</i>, significantly upregulated in A53T-PD1 iPSC line, showed interactions with extracellular matrix components and potential involvement in Wnt signaling. CCN3, also highly upregulated, demonstrated interactions with TP53, CDKN1A, and factors related to apoptosis and proliferation. <i>PTHLH</i>, while upregulated, exhibited interactions with CDK2 and genes involved in cell cycle regulation. RT-qPCR validation confirmed elevated <i>CCN3</i> and<i> PTHLH</i> expression in A53T-PD1 iPSCs, aligning with RNA-seq findings. These genes’ roles in preserving pluripotency and cellular stability require further exploration. In conclusion, we identified <i>CCN3, POSTN, </i>and <i>PTHLH</i> as potential contributors to genomic integrity and pluripotency maintenance in iPSCs. Their roles in DNA repair, apoptosis evasion, and signaling pathways could offer valuable insights for enhancing reprogramming efficiency and sustaining pluripotency. Further investigations are essential to unravel the mechanisms underlying their actions.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Molecular Biosciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fmolb.2024.1342011" target="_blank">https://dx.doi.org/10.3389/fmolb.2024.1342011</a></p>2024-02-05T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fmolb.2024.1342011https://figshare.com/articles/journal_contribution/_i_CCN3_i_i_POSTN_i_and_i_PTHLH_i_as_potential_key_regulators_of_genomic_integrity_and_cellular_survival_in_iPSCs/26403925CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/264039252024-02-05T03:00:00Z |
| spellingShingle | <i>CCN3</i>,<i> POSTN</i>, and <i>PTHLH</i> as potential key regulators of genomic integrity and cellular survival in iPSCs Nuha T. Swaidan (18595219) Biological sciences Genetics Biomedical and clinical sciences Clinical sciences iPSCs ESCs transcription factors genomic integrity cellular survival |
| status_str | publishedVersion |
| title | <i>CCN3</i>,<i> POSTN</i>, and <i>PTHLH</i> as potential key regulators of genomic integrity and cellular survival in iPSCs |
| title_full | <i>CCN3</i>,<i> POSTN</i>, and <i>PTHLH</i> as potential key regulators of genomic integrity and cellular survival in iPSCs |
| title_fullStr | <i>CCN3</i>,<i> POSTN</i>, and <i>PTHLH</i> as potential key regulators of genomic integrity and cellular survival in iPSCs |
| title_full_unstemmed | <i>CCN3</i>,<i> POSTN</i>, and <i>PTHLH</i> as potential key regulators of genomic integrity and cellular survival in iPSCs |
| title_short | <i>CCN3</i>,<i> POSTN</i>, and <i>PTHLH</i> as potential key regulators of genomic integrity and cellular survival in iPSCs |
| title_sort | <i>CCN3</i>,<i> POSTN</i>, and <i>PTHLH</i> as potential key regulators of genomic integrity and cellular survival in iPSCs |
| topic | Biological sciences Genetics Biomedical and clinical sciences Clinical sciences iPSCs ESCs transcription factors genomic integrity cellular survival |