Mass spectrometry-based proteomic profiling of extracellular vesicle proteins in diabetic and non-diabetic ischemic stroke patients: a case-control study
<p dir="ltr">Acute ischemic stroke is the most common cause of neurologic dysfunction caused by focal brain ischemia and tissue injury. Diabetes is a major risk factor of stroke, exacerbating disease management and prognosis. Therefore, discovering new diagnostic markers and therapeu...
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2024
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| _version_ | 1864513509704859648 |
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| author | Shahnaz Qadri (2610634) |
| author2 | Muhamad U. Sohail (18827248) Naveed Akhtar (4919398) Ghulam Jeelani Pir (13914063) Ghada Yousif (18827251) Sajitha V. Pananchikkal (13914060) Muna Al-Noubi (18827254) Sunkyu Choi (2141545) Ashfaq Shuaib (4919407) Yousef Haik (2610628) Aijaz Parray (290256) Frank Schmidt (207186) |
| author2_role | author author author author author author author author author author author |
| author_facet | Shahnaz Qadri (2610634) Muhamad U. Sohail (18827248) Naveed Akhtar (4919398) Ghulam Jeelani Pir (13914063) Ghada Yousif (18827251) Sajitha V. Pananchikkal (13914060) Muna Al-Noubi (18827254) Sunkyu Choi (2141545) Ashfaq Shuaib (4919407) Yousef Haik (2610628) Aijaz Parray (290256) Frank Schmidt (207186) |
| author_role | author |
| dc.creator.none.fl_str_mv | Shahnaz Qadri (2610634) Muhamad U. Sohail (18827248) Naveed Akhtar (4919398) Ghulam Jeelani Pir (13914063) Ghada Yousif (18827251) Sajitha V. Pananchikkal (13914060) Muna Al-Noubi (18827254) Sunkyu Choi (2141545) Ashfaq Shuaib (4919407) Yousef Haik (2610628) Aijaz Parray (290256) Frank Schmidt (207186) |
| dc.date.none.fl_str_mv | 2024-06-14T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fmolb.2024.1387859 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Mass_spectrometry-based_proteomic_profiling_of_extracellular_vesicle_proteins_in_diabetic_and_non-diabetic_ischemic_stroke_patients_a_case-control_study/26491042 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Cardiovascular medicine and haematology Medical biochemistry and metabolomics Neurosciences extracellular vesicles (EVs) diabetes stroke exosomes mass spectrometry blood coagulation tissue injury complement system |
| dc.title.none.fl_str_mv | Mass spectrometry-based proteomic profiling of extracellular vesicle proteins in diabetic and non-diabetic ischemic stroke patients: a case-control study |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Acute ischemic stroke is the most common cause of neurologic dysfunction caused by focal brain ischemia and tissue injury. Diabetes is a major risk factor of stroke, exacerbating disease management and prognosis. Therefore, discovering new diagnostic markers and therapeutic targets is critical for stroke prevention and treatment. Extracellular vesicles (EVs), with their distinctive properties, have emerged as promising candidates for biomarker discovery and therapeutic application. This case-control study utilized mass spectrometry-based proteomics to compare EVs from non-diabetic stroke (nDS = 14), diabetic stroke (DS = 13), and healthy control (HC = 12) subjects. Among 1288 identified proteins, 387 were statistically compared. Statistical comparisons using a general linear model (log2 foldchange ≥0.58 and FDR-<i>p</i>≤0.05) were performed for nDS vs HC, DS vs HC, and DS vs nDS. DS vs HC and DS vs nDS comparisons produced 123 and 149 differentially expressed proteins, respectively. Fibrinogen gamma chain (FIBG), Fibrinogen beta chain (FIBB), Tetratricopeptide repeat protein 16 (TTC16), Proline rich 14-like (PR14L), Inhibitor of nuclear factor kappa-B kinase subunit epsilon (IKKE), Biorientation of chromosomes in cell division protein 1-like 1 (BD1L1), and protein PR14L exhibited significant differences in the DS group. The pathway analysis revealed that the complement system pathways were activated, and blood coagulation and neuroprotection were inhibited in the DS group (z-score ≥2; <i>p </i>≤ 0.05). These findings underscore the potential of EVs proteomics in identifying biomarkers for stroke management and prevention, warranting further clinical investigation.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Molecular Biosciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fmolb.2024.1387859" target="_blank">https://dx.doi.org/10.3389/fmolb.2024.1387859</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_ff055375a15862c49ef25ff0a939338f |
| identifier_str_mv | 10.3389/fmolb.2024.1387859 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26491042 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Mass spectrometry-based proteomic profiling of extracellular vesicle proteins in diabetic and non-diabetic ischemic stroke patients: a case-control studyShahnaz Qadri (2610634)Muhamad U. Sohail (18827248)Naveed Akhtar (4919398)Ghulam Jeelani Pir (13914063)Ghada Yousif (18827251)Sajitha V. Pananchikkal (13914060)Muna Al-Noubi (18827254)Sunkyu Choi (2141545)Ashfaq Shuaib (4919407)Yousef Haik (2610628)Aijaz Parray (290256)Frank Schmidt (207186)Biomedical and clinical sciencesCardiovascular medicine and haematologyMedical biochemistry and metabolomicsNeurosciencesextracellular vesicles (EVs)diabetesstrokeexosomesmass spectrometryblood coagulationtissue injurycomplement system<p dir="ltr">Acute ischemic stroke is the most common cause of neurologic dysfunction caused by focal brain ischemia and tissue injury. Diabetes is a major risk factor of stroke, exacerbating disease management and prognosis. Therefore, discovering new diagnostic markers and therapeutic targets is critical for stroke prevention and treatment. Extracellular vesicles (EVs), with their distinctive properties, have emerged as promising candidates for biomarker discovery and therapeutic application. This case-control study utilized mass spectrometry-based proteomics to compare EVs from non-diabetic stroke (nDS = 14), diabetic stroke (DS = 13), and healthy control (HC = 12) subjects. Among 1288 identified proteins, 387 were statistically compared. Statistical comparisons using a general linear model (log2 foldchange ≥0.58 and FDR-<i>p</i>≤0.05) were performed for nDS vs HC, DS vs HC, and DS vs nDS. DS vs HC and DS vs nDS comparisons produced 123 and 149 differentially expressed proteins, respectively. Fibrinogen gamma chain (FIBG), Fibrinogen beta chain (FIBB), Tetratricopeptide repeat protein 16 (TTC16), Proline rich 14-like (PR14L), Inhibitor of nuclear factor kappa-B kinase subunit epsilon (IKKE), Biorientation of chromosomes in cell division protein 1-like 1 (BD1L1), and protein PR14L exhibited significant differences in the DS group. The pathway analysis revealed that the complement system pathways were activated, and blood coagulation and neuroprotection were inhibited in the DS group (z-score ≥2; <i>p </i>≤ 0.05). These findings underscore the potential of EVs proteomics in identifying biomarkers for stroke management and prevention, warranting further clinical investigation.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Molecular Biosciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fmolb.2024.1387859" target="_blank">https://dx.doi.org/10.3389/fmolb.2024.1387859</a></p>2024-06-14T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fmolb.2024.1387859https://figshare.com/articles/journal_contribution/Mass_spectrometry-based_proteomic_profiling_of_extracellular_vesicle_proteins_in_diabetic_and_non-diabetic_ischemic_stroke_patients_a_case-control_study/26491042CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/264910422024-06-14T09:00:00Z |
| spellingShingle | Mass spectrometry-based proteomic profiling of extracellular vesicle proteins in diabetic and non-diabetic ischemic stroke patients: a case-control study Shahnaz Qadri (2610634) Biomedical and clinical sciences Cardiovascular medicine and haematology Medical biochemistry and metabolomics Neurosciences extracellular vesicles (EVs) diabetes stroke exosomes mass spectrometry blood coagulation tissue injury complement system |
| status_str | publishedVersion |
| title | Mass spectrometry-based proteomic profiling of extracellular vesicle proteins in diabetic and non-diabetic ischemic stroke patients: a case-control study |
| title_full | Mass spectrometry-based proteomic profiling of extracellular vesicle proteins in diabetic and non-diabetic ischemic stroke patients: a case-control study |
| title_fullStr | Mass spectrometry-based proteomic profiling of extracellular vesicle proteins in diabetic and non-diabetic ischemic stroke patients: a case-control study |
| title_full_unstemmed | Mass spectrometry-based proteomic profiling of extracellular vesicle proteins in diabetic and non-diabetic ischemic stroke patients: a case-control study |
| title_short | Mass spectrometry-based proteomic profiling of extracellular vesicle proteins in diabetic and non-diabetic ischemic stroke patients: a case-control study |
| title_sort | Mass spectrometry-based proteomic profiling of extracellular vesicle proteins in diabetic and non-diabetic ischemic stroke patients: a case-control study |
| topic | Biomedical and clinical sciences Cardiovascular medicine and haematology Medical biochemistry and metabolomics Neurosciences extracellular vesicles (EVs) diabetes stroke exosomes mass spectrometry blood coagulation tissue injury complement system |