The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome

<p dir="ltr">Our study objective was to assess the effect of various sperm DNA fragmentation levels on clinical intracytoplasmic sperm injection outcome. This retrospective study included 392 patients who underwent ICSI and performed sperm DNA fragmentation testing before the procedu...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Kareim Khalafalla (14148807) (author)
مؤلفون آخرون: Ahmad Majzoub (14058045) (author), Haitham Elbardisi (14058048) (author), Ashni Bhathella (14779228) (author), Arth Chaudhari (14779231) (author), Ashok Agarwal (325619) (author), Ralf Henkel (8192964) (author), Thoraya AlMarzooki (14779234) (author), Hasan Burjaq (13036446) (author), Mohamed Arafa (14058042) (author)
منشور في: 2021
الموضوعات:
الوسوم: إضافة وسم
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author Kareim Khalafalla (14148807)
author2 Ahmad Majzoub (14058045)
Haitham Elbardisi (14058048)
Ashni Bhathella (14779228)
Arth Chaudhari (14779231)
Ashok Agarwal (325619)
Ralf Henkel (8192964)
Thoraya AlMarzooki (14779234)
Hasan Burjaq (13036446)
Mohamed Arafa (14058042)
author2_role author
author
author
author
author
author
author
author
author
author_facet Kareim Khalafalla (14148807)
Ahmad Majzoub (14058045)
Haitham Elbardisi (14058048)
Ashni Bhathella (14779228)
Arth Chaudhari (14779231)
Ashok Agarwal (325619)
Ralf Henkel (8192964)
Thoraya AlMarzooki (14779234)
Hasan Burjaq (13036446)
Mohamed Arafa (14058042)
author_role author
dc.creator.none.fl_str_mv Kareim Khalafalla (14148807)
Ahmad Majzoub (14058045)
Haitham Elbardisi (14058048)
Ashni Bhathella (14779228)
Arth Chaudhari (14779231)
Ashok Agarwal (325619)
Ralf Henkel (8192964)
Thoraya AlMarzooki (14779234)
Hasan Burjaq (13036446)
Mohamed Arafa (14058042)
dc.date.none.fl_str_mv 2021-07-11T06:00:00Z
dc.identifier.none.fl_str_mv 10.1111/and.14180
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/The_effect_of_sperm_DNA_fragmentation_on_intracytoplasmic_sperm_injection_outcome/22258438
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biological sciences
Genetics
Biomedical and clinical sciences
Clinical sciences
Reproductive medicine
clinical reproductive outcomes
intracytoplasmic sperm injection
sperm chromatin dispersion test
sperm DNA fragmentation
dc.title.none.fl_str_mv The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Our study objective was to assess the effect of various sperm DNA fragmentation levels on clinical intracytoplasmic sperm injection outcome. This retrospective study included 392 patients who underwent ICSI and performed sperm DNA fragmentation testing before the procedure. Based on sperm DNA fragmentation cut-off values, the patients were differentiated into 3 groups as <20%, 20%–30% and >30%. According to the female status, patients were differentiated into favourable group (<i>n</i> = 259) with female age <35 years and anti-Mullerian hormone level ≥7.1 pmol/L; and unfavourable group (<i>n</i> = 133) with female age ≥35 years and anti-Mullerian hormone level ≤7.1 pmol/L. The patient's medical records were reviewed, and patient's demographic, laboratory data including semen analysis, sperm DNA fragmentation determined by means of sperm chromatin dispersion, hormonal profile and data regarding intracytoplasmic sperm injection cycle were collected. This cohort reported that the clinical reproductive outcomes of intracytoplasmic sperm injection showed no statistical significance with increase sperm DNA fragmentation levels. In sperm DNA fragmentation above 30%, favourable females had significantly higher clinical pregnancy rate and live birth rate than unfavourable females, while fertilisation rate and miscarriage rate showed no significance between the subgroups. High sperm DNA fragmentation is linked to poor semen parameters.</p><h2>Other Information</h2><p dir="ltr">Published in: Andrologia<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1111/and.14180" target="_blank">http://dx.doi.org/10.1111/and.14180</a></p>
eu_rights_str_mv openAccess
id Manara2_ff4402a499a7618c4bbceee990674790
identifier_str_mv 10.1111/and.14180
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/22258438
publishDate 2021
repository.mail.fl_str_mv
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rights_invalid_str_mv CC BY 4.0
spelling The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcomeKareim Khalafalla (14148807)Ahmad Majzoub (14058045)Haitham Elbardisi (14058048)Ashni Bhathella (14779228)Arth Chaudhari (14779231)Ashok Agarwal (325619)Ralf Henkel (8192964)Thoraya AlMarzooki (14779234)Hasan Burjaq (13036446)Mohamed Arafa (14058042)Biological sciencesGeneticsBiomedical and clinical sciencesClinical sciencesReproductive medicineclinical reproductive outcomesintracytoplasmic sperm injectionsperm chromatin dispersion testsperm DNA fragmentation<p dir="ltr">Our study objective was to assess the effect of various sperm DNA fragmentation levels on clinical intracytoplasmic sperm injection outcome. This retrospective study included 392 patients who underwent ICSI and performed sperm DNA fragmentation testing before the procedure. Based on sperm DNA fragmentation cut-off values, the patients were differentiated into 3 groups as <20%, 20%–30% and >30%. According to the female status, patients were differentiated into favourable group (<i>n</i> = 259) with female age <35 years and anti-Mullerian hormone level ≥7.1 pmol/L; and unfavourable group (<i>n</i> = 133) with female age ≥35 years and anti-Mullerian hormone level ≤7.1 pmol/L. The patient's medical records were reviewed, and patient's demographic, laboratory data including semen analysis, sperm DNA fragmentation determined by means of sperm chromatin dispersion, hormonal profile and data regarding intracytoplasmic sperm injection cycle were collected. This cohort reported that the clinical reproductive outcomes of intracytoplasmic sperm injection showed no statistical significance with increase sperm DNA fragmentation levels. In sperm DNA fragmentation above 30%, favourable females had significantly higher clinical pregnancy rate and live birth rate than unfavourable females, while fertilisation rate and miscarriage rate showed no significance between the subgroups. High sperm DNA fragmentation is linked to poor semen parameters.</p><h2>Other Information</h2><p dir="ltr">Published in: Andrologia<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1111/and.14180" target="_blank">http://dx.doi.org/10.1111/and.14180</a></p>2021-07-11T06:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1111/and.14180https://figshare.com/articles/journal_contribution/The_effect_of_sperm_DNA_fragmentation_on_intracytoplasmic_sperm_injection_outcome/22258438CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/222584382021-07-11T06:00:00Z
spellingShingle The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome
Kareim Khalafalla (14148807)
Biological sciences
Genetics
Biomedical and clinical sciences
Clinical sciences
Reproductive medicine
clinical reproductive outcomes
intracytoplasmic sperm injection
sperm chromatin dispersion test
sperm DNA fragmentation
status_str publishedVersion
title The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome
title_full The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome
title_fullStr The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome
title_full_unstemmed The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome
title_short The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome
title_sort The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome
topic Biological sciences
Genetics
Biomedical and clinical sciences
Clinical sciences
Reproductive medicine
clinical reproductive outcomes
intracytoplasmic sperm injection
sperm chromatin dispersion test
sperm DNA fragmentation