Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein
<h3>Background</h3><p dir="ltr">The role of cerebrospinal fluid (CSF) alpha-synuclein as a potential biomarker has been challenged mainly due to variable preanalytical measures between laboratories. To evaluate the impact of the preanalytical factors contributing to such...
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| مؤلفون آخرون: | , , , , , |
| منشور في: |
2021
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| _version_ | 1864513515358781440 |
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| author | Ilham Y. Abdi (14778898) |
| author2 | Nour K. Majbour (8809316) Eline A. J. Willemse (9939754) Wilma D. J. van de Berg (8809325) Brit Mollenhauer (482499) Charlotte E. Teunissen (7830647) Omar M. El-Agnaf (3172641) |
| author2_role | author author author author author author |
| author_facet | Ilham Y. Abdi (14778898) Nour K. Majbour (8809316) Eline A. J. Willemse (9939754) Wilma D. J. van de Berg (8809325) Brit Mollenhauer (482499) Charlotte E. Teunissen (7830647) Omar M. El-Agnaf (3172641) |
| author_role | author |
| dc.creator.none.fl_str_mv | Ilham Y. Abdi (14778898) Nour K. Majbour (8809316) Eline A. J. Willemse (9939754) Wilma D. J. van de Berg (8809325) Brit Mollenhauer (482499) Charlotte E. Teunissen (7830647) Omar M. El-Agnaf (3172641) |
| dc.date.none.fl_str_mv | 2021-03-03T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fnagi.2021.638718 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Preanalytical_Stability_of_CSF_Total_and_Oligomeric_Alpha-Synuclein/25867651 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Neurosciences preanalytical factors alpha-synuclein oligomers cerebrospinal fluid biomarkers |
| dc.title.none.fl_str_mv | Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Background</h3><p dir="ltr">The role of cerebrospinal fluid (CSF) alpha-synuclein as a potential biomarker has been challenged mainly due to variable preanalytical measures between laboratories. To evaluate the impact of the preanalytical factors contributing to such variability, the different subforms of alpha-synuclein need to be studied individually.</p><p><br></p><h3>Method</h3><p dir="ltr">We investigated the effect of exposing CSF samples to several preanalytical sources of variability: (1) different polypropylene (PP) storage tubes; (2) use of non-ionic detergents; (3) multiple tube transfers; (4) multiple freeze-thaw cycles; and (5) delayed storage. CSF oligomeric- and total-alpha-synuclein levels were estimated using our in-house sandwich-based enzyme-linked immunosorbent assays.</p><p><br></p><h3>Results</h3><p dir="ltr">Siliconized tubes provided the optimal preservation of CSF alpha-synuclein proteins among other tested polypropylene tubes. The use of tween-20 detergent significantly improved the recovery of oligomeric-alpha-synuclein, while multiple freeze-thaw cycles significantly lowered oligomeric-alpha-synuclein in CSF. Interestingly, oligomeric-alpha-synuclein levels remained relatively stable over multiple tube transfers and upon delayed storage.</p><p><br></p><h3>Conclusion</h3><p dir="ltr">Our study showed for the first-time distinct impact of preanalytical factors on the different forms of CSF alpha-synuclein. These findings highlight the need for special considerations for the different forms of alpha-synuclein during CSF samples’ collection and processing.</p><p dir="ltr"><br></p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Aging Neuroscience<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fnagi.2021.638718" target="_blank">https://dx.doi.org/10.3389/fnagi.2021.638718</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_ff501b679afe85cd70c5c313bc044a09 |
| identifier_str_mv | 10.3389/fnagi.2021.638718 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25867651 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Preanalytical Stability of CSF Total and Oligomeric Alpha-SynucleinIlham Y. Abdi (14778898)Nour K. Majbour (8809316)Eline A. J. Willemse (9939754)Wilma D. J. van de Berg (8809325)Brit Mollenhauer (482499)Charlotte E. Teunissen (7830647)Omar M. El-Agnaf (3172641)Biomedical and clinical sciencesNeurosciencespreanalytical factorsalpha-synucleinoligomerscerebrospinal fluidbiomarkers<h3>Background</h3><p dir="ltr">The role of cerebrospinal fluid (CSF) alpha-synuclein as a potential biomarker has been challenged mainly due to variable preanalytical measures between laboratories. To evaluate the impact of the preanalytical factors contributing to such variability, the different subforms of alpha-synuclein need to be studied individually.</p><p><br></p><h3>Method</h3><p dir="ltr">We investigated the effect of exposing CSF samples to several preanalytical sources of variability: (1) different polypropylene (PP) storage tubes; (2) use of non-ionic detergents; (3) multiple tube transfers; (4) multiple freeze-thaw cycles; and (5) delayed storage. CSF oligomeric- and total-alpha-synuclein levels were estimated using our in-house sandwich-based enzyme-linked immunosorbent assays.</p><p><br></p><h3>Results</h3><p dir="ltr">Siliconized tubes provided the optimal preservation of CSF alpha-synuclein proteins among other tested polypropylene tubes. The use of tween-20 detergent significantly improved the recovery of oligomeric-alpha-synuclein, while multiple freeze-thaw cycles significantly lowered oligomeric-alpha-synuclein in CSF. Interestingly, oligomeric-alpha-synuclein levels remained relatively stable over multiple tube transfers and upon delayed storage.</p><p><br></p><h3>Conclusion</h3><p dir="ltr">Our study showed for the first-time distinct impact of preanalytical factors on the different forms of CSF alpha-synuclein. These findings highlight the need for special considerations for the different forms of alpha-synuclein during CSF samples’ collection and processing.</p><p dir="ltr"><br></p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Aging Neuroscience<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fnagi.2021.638718" target="_blank">https://dx.doi.org/10.3389/fnagi.2021.638718</a></p>2021-03-03T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fnagi.2021.638718https://figshare.com/articles/journal_contribution/Preanalytical_Stability_of_CSF_Total_and_Oligomeric_Alpha-Synuclein/25867651CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/258676512021-03-03T03:00:00Z |
| spellingShingle | Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein Ilham Y. Abdi (14778898) Biomedical and clinical sciences Neurosciences preanalytical factors alpha-synuclein oligomers cerebrospinal fluid biomarkers |
| status_str | publishedVersion |
| title | Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein |
| title_full | Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein |
| title_fullStr | Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein |
| title_full_unstemmed | Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein |
| title_short | Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein |
| title_sort | Preanalytical Stability of CSF Total and Oligomeric Alpha-Synuclein |
| topic | Biomedical and clinical sciences Neurosciences preanalytical factors alpha-synuclein oligomers cerebrospinal fluid biomarkers |