Application of STUPPIT to identify novel intermediary proteins between SMAD4 and β-catenin, central hubs of BMP and Wnt pathway.

(A) Schematic of the opposing effects of BMP and Wnt signaling on stem cell behavior. (B) STUPPIT strategy for labeling proteins that bridge SMAD4 and β-catenin. (C) STUPPIT workflow for capturing intermediary proteins between SMAD4 a...

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Hlavní autor:	Lin Xie (136792) (author)
Další autoři:	Lijuan Gao (5093777) (author), Weihong Fu (8430690) (author), Gangyun Wu (22676687) (author), Hua Li (46469) (author), Wenxiu Ning (18239607) (author)
Vydáno:	2025
Témata:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Physiology Biotechnology Developmental Biology Cancer Inorganic Chemistry Infectious Diseases Plant Biology Virology Chemical Sciences not elsewhere classified step enzymatic reaction offering new insights capturing intermediary proteins capture intermediary proteins biotinylate intermediary proteins plit -< u div >< p labeling intermediary proteins tu </ u proximity labeling tools proximity labeling tool characterized protein triads bmp signaling pathways p </ u bridge two non </ u signaling pathways interacting proteins tools available powerful tool two non protein interactions key components interacting partners identifying upstream downstream effectors disease pathogenesis directly label
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Application of STUPPIT to identify novel intermediary proteins between SMAD4 and β-catenin, central hubs of BMP and Wnt pathway.

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