The 3D tumor spheroid killing effect of combined CD3/GD2 BiTE and Vγ9Vδ2 T cells.

The 3D tumor spheroid killing effect of combined CD3/GD2 BiTE and Vγ9Vδ2 T cells.

<p>(A) Fluorescence images of 3D tumor spheroids treated with CD3/GD2 BiTE-armed Vγ9Vδ2 T cells. (B) The CTCF fold change of GFP and EthD-1 in 3D tumor spheroids treated with CD3/GD2 BiTE-armed Vγ9Vδ2 T cells. The 3D tumor spheroids of neuroblastoma were generated using SH-SY5Y GFP cells. CD3/...

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Main Author: Kuntida Kitidee (3935783) (author)
Other Authors: Sumet Amonyingcharoen (21512526) (author), Sarinthip Preedagasamzin (15332756) (author), Korakot Atjanasuppat (3821833) (author), Piamsiri Sawaisorn (14608498) (author), Pornprapa Srimorkun (21512529) (author), Sawang Petvises (523491) (author), Wanpen Chaicumpa (17781) (author), Suparerk Borwornpinyo (3509102) (author), Usanarat Anurathapan (4570963) (author), Suradej Hongeng (285857) (author)
Published: 2025
Subjects:
Biophysics
Biochemistry
Cell Biology
Molecular Biology
Neuroscience
Physiology
Pharmacology
Biotechnology
Immunology
Developmental Biology
Cancer
Hematology
Infectious Diseases
Virology
Space Science
Biological Sciences not elsewhere classified
indirect elisa assay
flexible peptide linker
div >< p
compact monomeric structure
2 signal sequence
treating various types
gd bite armed
cells could represent
alternative immunotherapy strategy
alternative immunotherapy method
bind cd3 epsilon
treating neuroblastoma patients
gd2 bite combined
positive jurkat cells
negative raji cells
mouse scfv gd2
vh ), linked
neuroblastoma cell lines
dimensional cell cultures
combining bispecific antibody
treating neuroblastoma
gd2 bite
negative sk
nb ),
human scfv
combining cd3
bispecific antibody
neuroblastoma cells
sy5y cells
cells enabled
cell engager
gd2 bispecific
positive sh
vitro </
transiently produced
target antigens
sy5y gfp
strong potential
results suggest
protein secretion
promising approach
polyhistidine tag
intriguing targets
human vγ9vδ2
highest ability
high yield
cytotoxic abilities
binding specificity
based strategies
360 nm
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Summary:<p>(A) Fluorescence images of 3D tumor spheroids treated with CD3/GD2 BiTE-armed Vγ9Vδ2 T cells. (B) The CTCF fold change of GFP and EthD-1 in 3D tumor spheroids treated with CD3/GD2 BiTE-armed Vγ9Vδ2 T cells. The 3D tumor spheroids of neuroblastoma were generated using SH-SY5Y GFP cells. CD3/GD2 BiTE-armed Vγ9Vδ2 T cells were prepared and added to the 3D tumor spheroids at an E:T ratio of 1:1 for 48 h. Live cells expressed GFP, and dead cells were stained with EthD-1. The fold change of CTCF for GFP and EthD-1 was calculated relative to non-treated CD3/GD2 BiTE. Three independent experiments were performed. The results are plotted individually, and the means ± SD are presented. *<i>p</i> < 0.05, **<i>p</i> < 0.01.</p>

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