Validation of the intermediary proteins between β-catenin and SMAD4.
<p><b>(A–F)</b> Immunoprecipitation assays showing the interactions between RNF20 (A, D), ERC1 (B, E), and USP7 (C, F) with SMAD4 and β-catenin, respectively, as validated by Flag-IP. In these assays, HA-TbC was used as the control for panels A–C, while GFP-Flag was used as the con...
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2025
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