Validation of the intermediary proteins between β-catenin and SMAD4.

<p><b>(A–F)</b> Immunoprecipitation assays showing the interactions between RNF20 (A, D), ERC1 (B, E), and USP7 (C, F) with SMAD4 and β-catenin, respectively, as validated by Flag-IP. In these assays, HA-TbC was used as the control for panels A–C, while GFP-Flag was used as the con...

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Main Author:	Lin Xie (136792) (author)
Other Authors:	Lijuan Gao (5093777) (author), Weihong Fu (8430690) (author), Gangyun Wu (22676687) (author), Hua Li (46469) (author), Wenxiu Ning (18239607) (author)
Published:	2025
Subjects:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Physiology Biotechnology Developmental Biology Cancer Inorganic Chemistry Infectious Diseases Plant Biology Virology Chemical Sciences not elsewhere classified step enzymatic reaction offering new insights capturing intermediary proteins capture intermediary proteins biotinylate intermediary proteins plit -< u div >< p labeling intermediary proteins tu </ u proximity labeling tools proximity labeling tool characterized protein triads bmp signaling pathways p </ u bridge two non </ u signaling pathways interacting proteins tools available powerful tool two non protein interactions key components interacting partners identifying upstream downstream effectors disease pathogenesis directly label
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Validation of the intermediary proteins between β-catenin and SMAD4.

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