Supplementary MovieS2b from Immunotherapeutic Targeting and PET Imaging of DLL3 in Small-Cell Neuroendocrine Prostate Cancer

Supplementary MovieS2b from Immunotherapeutic Targeting and PET Imaging of DLL3 in Small-Cell Neuroendocrine Prostate Cancer

<p>Supplemental Movies 2a-2c: 22Rv1-NuclightGreen and SHP-77-NuclightRed mixed population co-cultures. Time-lapse movies of 22Rv1 cells (labeled with NuclightGreen) and SHP-77 cells (labeled with NuclightRed) co-cultured with T cells. Both the 22Rv1 and SHP-77 cells were plated at equal densit...

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Hlavní autor: Jonathan Chou (14957015) (author)
Další autoři: Emily A. Egusa (14962343) (author), Sinan Wang (14962346) (author), Michelle L. Badura (14962349) (author), Fei Lee (14962352) (author), Anil P. Bidkar (14962355) (author), Jun Zhu (14962358) (author), Tanushree Shenoy (14957003) (author), Kai Trepka (14962361) (author), Troy M. Robinson (14962364) (author), Veronica Steri (14962367) (author), Jiaoti Huang (14962370) (author), Yuzhuo Wang (14962373) (author), Eric J. Small (14962376) (author), Emily Chan (14962379) (author), Bradley A. Stohr (14962382) (author), Alan Ashworth (14957030) (author), Brant Delafontaine (14962385) (author), Sylvie Rottey (14962388) (author), Keegan S. Cooke (14962391) (author), Nooshin Hashemi Sadraei (14962394) (author), Brian Yu (14962397) (author), Mark Salvati (14962400) (author), Julie M. Bailis (14962403) (author), Felix Y. Feng (14845891) (author), Robert R. Flavell (9563200) (author), Rahul Aggarwal (14960826) (author)
Vydáno: 2025
Témata:
Cancer
Therapeutic Research and Development
Immuno-oncology
Methods and Technology
Drug Targets
Cell surface receptor drug targets
Genitourinary Cancers
Prostate cancer
Imaging
Positron emission tomography (PET)
Immunotherapy
Preclinical Models
Xenograft models
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Shrnutí:<p>Supplemental Movies 2a-2c: 22Rv1-NuclightGreen and SHP-77-NuclightRed mixed population co-cultures. Time-lapse movies of 22Rv1 cells (labeled with NuclightGreen) and SHP-77 cells (labeled with NuclightRed) co-cultured with T cells. Both the 22Rv1 and SHP-77 cells were plated at equal densities and treated with either NT control BiTE®, AMG 757, or the PSMA-targeting HLE BiTE, AMG 160. T cells were added to each well at an E:T ratio of 10:1. Images were captured every 4 hours at 4x magnification for 5 days. a.22Rv1, SHP-77, and T cells co-cultured with NT control BiTE®. b.22Rv1, SHP-77, and T cells co-cultured with AMG 757. c.22Rv1, SHP-77, and T cells co-cultured with AMG 160.</p>

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