Table 1_Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections.docx
Introduction<p>Vibrio species are widespread in coastal environments, where they are being increasingly associated with mortality episodes of farmed bivalves. This is the case for Vibrio ostreicida strain r172 isolated from a mortality event of adult mussels Mytilus galloprovincialis in Spain...
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2025
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| _version_ | 1849927624716976128 |
|---|---|
| author | Martina Leonessi (22686236) |
| author2 | Jose R. Lopez (8470305) Yanis Cruz-Quintana (8822450) Manon Auguste (8710872) Karl B. Andree (10097092) Margarita Fernandez-Tejedor (22686401) Luigi Vezzulli (426915) Dolors Furones (9581414) Laura Canesi (166198) |
| author2_role | author author author author author author author author |
| author_facet | Martina Leonessi (22686236) Jose R. Lopez (8470305) Yanis Cruz-Quintana (8822450) Manon Auguste (8710872) Karl B. Andree (10097092) Margarita Fernandez-Tejedor (22686401) Luigi Vezzulli (426915) Dolors Furones (9581414) Laura Canesi (166198) |
| author_role | author |
| dc.creator.none.fl_str_mv | Martina Leonessi (22686236) Jose R. Lopez (8470305) Yanis Cruz-Quintana (8822450) Manon Auguste (8710872) Karl B. Andree (10097092) Margarita Fernandez-Tejedor (22686401) Luigi Vezzulli (426915) Dolors Furones (9581414) Laura Canesi (166198) |
| dc.date.none.fl_str_mv | 2025-11-26T05:14:46Z |
| dc.identifier.none.fl_str_mv | 10.3389/fimmu.2025.1727164.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Table_1_Dynamics_of_Vibrio_ostreicida_in_Mytilus_galloprovincialis_through_in_vivo_infections_docx/30717602 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Genetic Immunology V. ostreicida isolate mussel mortality cohabitation infection bath infection PCR horizontal transmission hemolymph responses histopathology |
| dc.title.none.fl_str_mv | Table 1_Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections.docx |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | Introduction<p>Vibrio species are widespread in coastal environments, where they are being increasingly associated with mortality episodes of farmed bivalves. This is the case for Vibrio ostreicida strain r172 isolated from a mortality event of adult mussels Mytilus galloprovincialis in Spain in 2022.</p>Methods and Results<p>In this study, the infection dynamics and immune responses of adult M. galloprovincialis challenged with V. ostreicida r172 were investigated using different in vivo experimental approaches. First, a virulence assay by injection (1 x 108 CFU/100 µL) was performed at different temperatures (12, 18 and 24 °C). The results showed that mussel mortality (about 50 % within 8 days) was independent of increasing temperatures. Subsequently, an injection/cohabitation experiment was carried out placing together in the same tank V. ostreicida-injected (Donors, 108 CFU/mL) with un-injected mussels (Recipients). The time course of infection was then followed by evaluating positivity to V. ostreicida by PCR, responses of haemolymph components (haemocyte lysosomal membrane stability and serum lysozyme activity) and tissue histopathology (gills and digestive gland). The results showed a partial horizontal transfer of V. ostreicida from infected to uninfected mussels, with transient effects on haemolymph responses and histopathological lesions in both groups. Finally, in order to mimic more realistic environmental conditions, a bath infection experiment was carried out, exposing mussels to V. ostreicida in seawater (105 CFU/mL). This condition resulted in lower stress in haemocytes; moreover, no lysozyme release or histopathological alterations were observed.</p>Discussion<p>Overall, the results show that M. galloprovincialis is able to cope with challenge with V. ostreicida, indicating that this Vibrio species is moderately pathogenic to adult mussels under the established experimental conditions.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_0c055e11fe1fca4b8586c35f2cee7af6 |
| identifier_str_mv | 10.3389/fimmu.2025.1727164.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30717602 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Table 1_Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections.docxMartina Leonessi (22686236)Jose R. Lopez (8470305)Yanis Cruz-Quintana (8822450)Manon Auguste (8710872)Karl B. Andree (10097092)Margarita Fernandez-Tejedor (22686401)Luigi Vezzulli (426915)Dolors Furones (9581414)Laura Canesi (166198)Genetic ImmunologyV. ostreicida isolatemussel mortalitycohabitation infectionbath infectionPCRhorizontal transmissionhemolymph responseshistopathologyIntroduction<p>Vibrio species are widespread in coastal environments, where they are being increasingly associated with mortality episodes of farmed bivalves. This is the case for Vibrio ostreicida strain r172 isolated from a mortality event of adult mussels Mytilus galloprovincialis in Spain in 2022.</p>Methods and Results<p>In this study, the infection dynamics and immune responses of adult M. galloprovincialis challenged with V. ostreicida r172 were investigated using different in vivo experimental approaches. First, a virulence assay by injection (1 x 108 CFU/100 µL) was performed at different temperatures (12, 18 and 24 °C). The results showed that mussel mortality (about 50 % within 8 days) was independent of increasing temperatures. Subsequently, an injection/cohabitation experiment was carried out placing together in the same tank V. ostreicida-injected (Donors, 108 CFU/mL) with un-injected mussels (Recipients). The time course of infection was then followed by evaluating positivity to V. ostreicida by PCR, responses of haemolymph components (haemocyte lysosomal membrane stability and serum lysozyme activity) and tissue histopathology (gills and digestive gland). The results showed a partial horizontal transfer of V. ostreicida from infected to uninfected mussels, with transient effects on haemolymph responses and histopathological lesions in both groups. Finally, in order to mimic more realistic environmental conditions, a bath infection experiment was carried out, exposing mussels to V. ostreicida in seawater (105 CFU/mL). This condition resulted in lower stress in haemocytes; moreover, no lysozyme release or histopathological alterations were observed.</p>Discussion<p>Overall, the results show that M. galloprovincialis is able to cope with challenge with V. ostreicida, indicating that this Vibrio species is moderately pathogenic to adult mussels under the established experimental conditions.</p>2025-11-26T05:14:46ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fimmu.2025.1727164.s001https://figshare.com/articles/dataset/Table_1_Dynamics_of_Vibrio_ostreicida_in_Mytilus_galloprovincialis_through_in_vivo_infections_docx/30717602CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307176022025-11-26T05:14:46Z |
| spellingShingle | Table 1_Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections.docx Martina Leonessi (22686236) Genetic Immunology V. ostreicida isolate mussel mortality cohabitation infection bath infection PCR horizontal transmission hemolymph responses histopathology |
| status_str | publishedVersion |
| title | Table 1_Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections.docx |
| title_full | Table 1_Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections.docx |
| title_fullStr | Table 1_Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections.docx |
| title_full_unstemmed | Table 1_Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections.docx |
| title_short | Table 1_Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections.docx |
| title_sort | Table 1_Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections.docx |
| topic | Genetic Immunology V. ostreicida isolate mussel mortality cohabitation infection bath infection PCR horizontal transmission hemolymph responses histopathology |