Addition of <i>NRG1</i> does not phenocopy Chr7 trisomy.
<p>(A) Diploid strains with an extra copy of <i>NRG1</i> were assayed for filamentation under hyphal-inducing conditions, liquid RPMI at 30°C, for three hours. At least 50 cells across 5 + fields of view were categorized as either yeast or filamentous for each strain per replicate...
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2025
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| Čoahkkáigeassu: | <p>(A) Diploid strains with an extra copy of <i>NRG1</i> were assayed for filamentation under hyphal-inducing conditions, liquid RPMI at 30°C, for three hours. At least 50 cells across 5 + fields of view were categorized as either yeast or filamentous for each strain per replicate and plotted as the average with standard error. Binomial GLMs for each strain background; *** for <i>p</i> < 0.001. N = 3. (B) Fungal cell adherence and uptake after co-incubation with OKF6/TERT-2 cells for 2.5 h and epithelial cell damage after 7 h were assayed. All boxplots represent the interquartile ranges with the median marked and whiskers extending to the outermost data points up to 1.5 times the interquartile range. One-way ANOVAs within the two strain backgrounds for each assay (** for <i>p</i> < 0.01, *** for <i>p</i> < 0.001). HPF = High Power Field. N = 9. (C) Ten C57BL/6 mice (5 male, 5 female) were injected via the tail vein with 5x10<sup>5</sup> <i>C. albicans</i> cells. Survival of the mice is indicated in the Kaplan-Meier curve and significance of pairwise comparisons was determined by Log-Rank test followed by Benjamini-Hochberg correction (p < 0.05 for Chr7x2 v. Chr7x3 (ABB) or Chr7x2 + NRG1).</p> |
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