Construction and expression of CD3/GD2 BiTE.

(A) Schematic diagram of CD3/GD2 BiTE in pcDNA3.1(+). CD3/GD2 BiTE consists of human scFv-CD3 clone 18 (VH-VL) linked to mouse scFv-GD2 (VL-VH) via a (G4S)3 linker. An Interleukin-2 signal peptide for protein secretion and a polyhistidine (His6) tag...

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Bibliographic Details
Main Author:	Kuntida Kitidee (3935783) (author)
Other Authors:	Sumet Amonyingcharoen (21512526) (author), Sarinthip Preedagasamzin (15332756) (author), Korakot Atjanasuppat (3821833) (author), Piamsiri Sawaisorn (14608498) (author), Pornprapa Srimorkun (21512529) (author), Sawang Petvises (523491) (author), Wanpen Chaicumpa (17781) (author), Suparerk Borwornpinyo (3509102) (author), Usanarat Anurathapan (4570963) (author), Suradej Hongeng (285857) (author)
Published:	2025
Subjects:	Biophysics Biochemistry Cell Biology Molecular Biology Neuroscience Physiology Pharmacology Biotechnology Immunology Developmental Biology Cancer Hematology Infectious Diseases Virology Space Science Biological Sciences not elsewhere classified indirect elisa assay flexible peptide linker div >< p compact monomeric structure 2 signal sequence treating various types gd bite armed cells could represent alternative immunotherapy strategy alternative immunotherapy method bind cd3 epsilon treating neuroblastoma patients gd2 bite combined positive jurkat cells negative raji cells mouse scfv gd2 vh ), linked neuroblastoma cell lines dimensional cell cultures combining bispecific antibody treating neuroblastoma gd2 bite negative sk nb ), human scfv combining cd3 bispecific antibody neuroblastoma cells sy5y cells cells enabled cell engager gd2 bispecific positive sh vitro </ transiently produced target antigens sy5y gfp strong potential results suggest protein secretion promising approach polyhistidine tag intriguing targets human vγ9vδ2 highest ability high yield cytotoxic abilities binding specificity based strategies 360 nm
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Construction and expression of CD3/GD2 BiTE.

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