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Purification of N-terminal His-tagged CHUA_005136 from 1.5 L of <i>Pichia pastoris</i> culture supernatant.

Purification of N-terminal His-tagged CHUA_005136 from 1.5 L of <i>Pichia pastoris</i> culture supernatant.

<p>Purification of N-terminal His-tagged CHUA_005136 from 1.5 L of <i>Pichia pastoris</i> culture supernatant.</p>

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Bibliografske podrobnosti
Glavni avtor: Takuya Yamaguchi (4543105) (author)
Drugi avtorji: Yasuhisa Asano (727606) (author)
Izdano: 2025
Teme:
Biochemistry
Evolutionary Biology
Ecology
Plant Biology
Virology
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
subsequently converting aldoxime
natural defensive chemicals
indicating sequential duplication
catalyse aldoxime dehydration
div >< p
cyanogenic millipedes evolved
chamberlinius hualienensis </
hualienensis </
r </
typically lineage
related enzymes
plant lineages
plant kingdoms
paralogous genes
millipedes demonstrates
mechanisms underlying
hydroxynitrile lyase
hydrogen cyanide
giving rise
enzymes independently
dependent monooxygenase
deeper understanding
cytochrome p450
complete set
coding genes
biological characterisation
>)- mandelonitrile
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  • Purification of N-terminal His-tagged CHUA_005137 from 1.5 L of <i>Pichia pastoris</i> culture supernatant.
    od: Takuya Yamaguchi (4543105)
    Izdano: (2025)
  • Purification of N-terminal His-tagged ChuaHNL (CHUA_005138) from 1.5 L of <i>Pichia pastoris</i> culture supernatant.
    od: Takuya Yamaguchi (4543105)
    Izdano: (2025)
  • Heterologous production of ChuaHNL and its paralogous proteins in <i>Pichia pastoris.</i>
    od: Takuya Yamaguchi (4543105)
    Izdano: (2025)
  • (<i>R</i>)-mandelonitrile synthetic reaction catalyzed by CHUA_005138 (ChuaHNL), CHUA_005137, and CHUA_005136.
    od: Takuya Yamaguchi (4543105)
    Izdano: (2025)
  • Substrate saturation curves for CHUA_005138 (ChuaHNL), CHUA_005137, and CHUA_005136 with (<i>R,S</i>)-mandelonitrile (mandelonitrile cleavage reaction) (A) or benzaldehyde ((<i>R</i>)-mandelonitrile synthetic reaction) (B).
    od: Takuya Yamaguchi (4543105)
    Izdano: (2025)

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