Simple steps to use the FastCloneAssist tool.

<p>To use the FastCloneAssist tool, start by running the program and selecting your preferred FastCloning method by entering 1 or 2. Next, input your DNA sequence in the specified format and optionally provide your desired melting temperature (Tm) value. Once you’ve entered the required inform...

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Bibliographic Details
Main Author:	Pradip Kumar Singh (20875050) (author)
Other Authors:	Michael S. Donnenberg (20875053) (author)
Published:	2025
Subjects:	Biochemistry Microbiology Genetics Molecular Biology Biotechnology Evolutionary Biology Developmental Biology Cancer Science Policy Infectious Diseases Plant Biology Biological Sciences not elsewhere classified xlink "> however xlink "> fastcloning step traditional methods optimizing primer design li et al friendly python program desired vector position desired melting temperature constructing fusion proteins revolutionizing molecular cloning powerful technique accessible failed cloning attempts efficient pcr amplification streamlined python tool minimal user input pcr cloning innovative technique accelerates cloning tm ), seamless integration present fastcloneassist paradigm shift manipulating genes introducing fastcloneassist insert sequences insert dna eliminating laborious dpni digestion critical hurdle chimeric cdnas
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Simple steps to use the FastCloneAssist tool.

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