The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells.
<p>Epidermal cells were agroinfiltrated with a GFP:p33 + p22, GFP:p33ΔN30 + p22, or just p22 VSR expressing construct and visualized at six dpi using an epifluorescence microscope. The images in the enlarged view column represent a close-up view of the regions within the box in the GFP column....
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2025
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| _version_ | 1849927641288671232 |
|---|---|
| author | Vicken Aknadibossian (22676694) |
| author2 | Clare Stokes (816556) Roger L. Papke (685990) Hao Wei Teh (22676697) Ying Wang (11406) Svetlana Y. Folimonova (8786570) |
| author2_role | author author author author author |
| author_facet | Vicken Aknadibossian (22676694) Clare Stokes (816556) Roger L. Papke (685990) Hao Wei Teh (22676697) Ying Wang (11406) Svetlana Y. Folimonova (8786570) |
| author_role | author |
| dc.creator.none.fl_str_mv | Vicken Aknadibossian (22676694) Clare Stokes (816556) Roger L. Papke (685990) Hao Wei Teh (22676697) Ying Wang (11406) Svetlana Y. Folimonova (8786570) |
| dc.date.none.fl_str_mv | 2025-11-24T18:34:58Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013730.g007 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/The_p33_deletion_mutant_GFP_p33_N30_is_localized_in_cytoplasmic_aggregates_compared_to_the_plasma_membrane_and_vesicle_localization_of_GFP_p33_when_ectopically_expressed_in_i_N_benthamiana_i_epidermal_cells_/30697740 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Medicine Microbiology Cell Biology Ecology Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified substantial structural similarities ion channel properties ion channel activity form ion channels closterovirus tristezae </ alphainfluenzavirus influenzae </ div >< p citrus tristeza virus lowered membrane potentials host ion homeostasis discuss additional characteristics m2 protein encoded viroporin </ p plant virus viroporin plant virus viroporins plant virus xenopus </ viroporin encoded influenza virus viral proteins using two using confocal third report study represents significantly lagged research focusing present evidence first validation favorable environments electron microscopy cytoplasmic domains associated protein >), possesses |
| dc.title.none.fl_str_mv | The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Epidermal cells were agroinfiltrated with a GFP:p33 + p22, GFP:p33ΔN30 + p22, or just p22 VSR expressing construct and visualized at six dpi using an epifluorescence microscope. The images in the enlarged view column represent a close-up view of the regions within the box in the GFP column. The scale bar represents 100 microns. VSR = viral suppressor of silencing (p22) only. BF = Brightfield.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_0dfca4a20e7f4ae977ab7c715f353962 |
| identifier_str_mv | 10.1371/journal.ppat.1013730.g007 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697740 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells.Vicken Aknadibossian (22676694)Clare Stokes (816556)Roger L. Papke (685990)Hao Wei Teh (22676697)Ying Wang (11406)Svetlana Y. Folimonova (8786570)BiophysicsBiochemistryMedicineMicrobiologyCell BiologyEcologyInfectious DiseasesPlant BiologyVirologyBiological Sciences not elsewhere classifiedsubstantial structural similaritiesion channel propertiesion channel activityform ion channelsclosterovirus tristezae </alphainfluenzavirus influenzae </div >< pcitrus tristeza viruslowered membrane potentialshost ion homeostasisdiscuss additional characteristicsm2 protein encodedviroporin </ pplant virus viroporinplant virus viroporinsplant virusxenopus </viroporin encodedinfluenza virusviral proteinsusing twousing confocalthird reportstudy representssignificantly laggedresearch focusingpresent evidencefirst validationfavorable environmentselectron microscopycytoplasmic domainsassociated protein>), possesses<p>Epidermal cells were agroinfiltrated with a GFP:p33 + p22, GFP:p33ΔN30 + p22, or just p22 VSR expressing construct and visualized at six dpi using an epifluorescence microscope. The images in the enlarged view column represent a close-up view of the regions within the box in the GFP column. The scale bar represents 100 microns. VSR = viral suppressor of silencing (p22) only. BF = Brightfield.</p>2025-11-24T18:34:58ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013730.g007https://figshare.com/articles/figure/The_p33_deletion_mutant_GFP_p33_N30_is_localized_in_cytoplasmic_aggregates_compared_to_the_plasma_membrane_and_vesicle_localization_of_GFP_p33_when_ectopically_expressed_in_i_N_benthamiana_i_epidermal_cells_/30697740CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306977402025-11-24T18:34:58Z |
| spellingShingle | The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells. Vicken Aknadibossian (22676694) Biophysics Biochemistry Medicine Microbiology Cell Biology Ecology Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified substantial structural similarities ion channel properties ion channel activity form ion channels closterovirus tristezae </ alphainfluenzavirus influenzae </ div >< p citrus tristeza virus lowered membrane potentials host ion homeostasis discuss additional characteristics m2 protein encoded viroporin </ p plant virus viroporin plant virus viroporins plant virus xenopus </ viroporin encoded influenza virus viral proteins using two using confocal third report study represents significantly lagged research focusing present evidence first validation favorable environments electron microscopy cytoplasmic domains associated protein >), possesses |
| status_str | publishedVersion |
| title | The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells. |
| title_full | The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells. |
| title_fullStr | The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells. |
| title_full_unstemmed | The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells. |
| title_short | The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells. |
| title_sort | The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells. |
| topic | Biophysics Biochemistry Medicine Microbiology Cell Biology Ecology Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified substantial structural similarities ion channel properties ion channel activity form ion channels closterovirus tristezae </ alphainfluenzavirus influenzae </ div >< p citrus tristeza virus lowered membrane potentials host ion homeostasis discuss additional characteristics m2 protein encoded viroporin </ p plant virus viroporin plant virus viroporins plant virus xenopus </ viroporin encoded influenza virus viral proteins using two using confocal third report study represents significantly lagged research focusing present evidence first validation favorable environments electron microscopy cytoplasmic domains associated protein >), possesses |