The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells.

The p33 deletion mutant GFP:p33ΔN30 is localized in cytoplasmic aggregates compared to the plasma membrane and vesicle localization of GFP:p33 when ectopically expressed in <i>N. benthamiana</i> epidermal cells.

<p>Epidermal cells were agroinfiltrated with a GFP:p33 + p22, GFP:p33ΔN30 + p22, or just p22 VSR expressing construct and visualized at six dpi using an epifluorescence microscope. The images in the enlarged view column represent a close-up view of the regions within the box in the GFP column....

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Wedi'i Gadw mewn:

Manylion Llyfryddiaeth
Prif Awdur:	Vicken Aknadibossian (22676694) (author)
Awduron Eraill:	Clare Stokes (816556) (author), Roger L. Papke (685990) (author), Hao Wei Teh (22676697) (author), Ying Wang (11406) (author), Svetlana Y. Folimonova (8786570) (author)
Cyhoeddwyd:	2025
Pynciau:	Biophysics Biochemistry Medicine Microbiology Cell Biology Ecology Infectious Diseases Plant Biology Virology Biological Sciences not elsewhere classified substantial structural similarities ion channel properties ion channel activity form ion channels closterovirus tristezae </ alphainfluenzavirus influenzae </ div >< p citrus tristeza virus lowered membrane potentials host ion homeostasis discuss additional characteristics m2 protein encoded viroporin </ p plant virus viroporin plant virus viroporins plant virus xenopus </ viroporin encoded influenza virus viral proteins using two using confocal third report study represents significantly lagged research focusing present evidence first validation favorable environments electron microscopy cytoplasmic domains associated protein >), possesses
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Eitemau Tebyg

The ectopic expression of GFP:p33 induced the accumulation of GFP:p33-containing vesicles and MVBs in <i>N. benthamiana</i> epidermal cells.
gan: Vicken Aknadibossian (22676694)
Cyhoeddwyd: (2025)

p33 displays cytoplasmic features of Class I viroporins.
gan: Vicken Aknadibossian (22676694)
Cyhoeddwyd: (2025)

Plasmolysis reveals novel p33 characteristics in <i>N. benthamiana</i> cells.
gan: Vicken Aknadibossian (22676694)
Cyhoeddwyd: (2025)

Western blot of purified p33 protein transiently expressed in <i>N. benthamiana</i> displaying protein bands which correspond to the expected sizes of p33 monomers, dimers, and tetramers.
gan: Vicken Aknadibossian (22676694)
Cyhoeddwyd: (2025)

Prediction of p33 TMD pore structure and surface hydrophobicity.
gan: Vicken Aknadibossian (22676694)
Cyhoeddwyd: (2025)

Structural Class I viroporin characteristics displayed by p33.
gan: Vicken Aknadibossian (22676694)
Cyhoeddwyd: (2025)

Functional characteristics shared among the CTV p33 and other known viroporins.
gan: Vicken Aknadibossian (22676694)
Cyhoeddwyd: (2025)

TEM observations of the vesicular structures and cell wall-plasma membrane interface ultrastructure of <i>N. benthamiana</i> epidermal cells in p33-expressing and control cells.
gan: Vicken Aknadibossian (22676694)
Cyhoeddwyd: (2025)

p33 exhibits marked ion channel activity in <i>Xenopus</i> oocytes.
gan: Vicken Aknadibossian (22676694)
Cyhoeddwyd: (2025)

Differential impact of EBOV and MARV on cAMP-mediated function in human gut organoids reveals region-specific loss of forskolin-induced swelling in HCOs.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

Transcriptomic profiling of HIOs reveals viral replication and distinct host responses to EBOV and MARV infection.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

iPSC-derived HIOs are permissive to EBOV and MARV infection.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

Delayed ISG induction in MARV infection and dysregulation of diarrhea-associated genes in EBOV- and MARV-infected human colonic organoids.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

Transcriptomic profiling of EBOV- and MARV-infected HCOs reveals viral load and shared and unique host responses.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

iPSC-derived HCOs are permissive to EBOV and MARV infection.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

iPSC-derived HIOs and HCOs are permissive to EBOV infection.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

Directed differentiation of human iPSCs into region-specific intestinal (HIOs) and colonic (HCOs) organoids.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

Disruption of organoid integrity and impaired forskolin response in PCOs infected with EBOV or MARV at high MOI.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

Selective colocalization of EBOV VP35 with enterocytes in PCOs.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

Experimental overview and infection dynamics of primary cell-derived intestinal colonic (PCOs) organoids.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

Virus- and region-specific epithelial immune activation in gut organoids at 3dpi.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

Viral transcript production in EBOV- and MARV-infected HCOs at 1 and 3days post-infection.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

BU310-Cre2 iPSC-derived HCOs are permissive to EBOV and MARV infection.
gan: Elizabeth Y. Flores (22676699)
Cyhoeddwyd: (2025)

Data and materials for: The Origins and Evolution of the RNA Virome in Land Flora
gan: Qiyan Liu (17898620)
Cyhoeddwyd: (2025)

Image 11_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Image 10_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Image 9_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Image 7_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Image 8_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Image 5_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Image 6_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Image 3_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Image 4_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Image 1_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Image 2_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.tif
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Data Sheet 5_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.pdf
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Data Sheet 6_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.pdf
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Data Sheet 3_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.pdf
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Data Sheet 4_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.pdf
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

Data Sheet 2_Synthetic DNA co-immunization with vaccine-aligned common consensus nucleoprotein and hemagglutinin protects mice against lethal influenza infection with a single immunization.pdf
gan: Ebony N. Gary (2019478)
Cyhoeddwyd: (2025)

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