Identification of TesA as the periplasmic partner of YbbAP.

<p><b>(A)</b> Pairwise <i><i>in silico</i></i> co-folding of YbbP with all sec/tat secreted proteins from <i><i>Escherichia coli</i></i>. Each co-fold attempt is represented on the 2D plot by their pDockQ score and Molprobity clash sc...

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में बचाया:
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मुख्य लेखक: Martin B. L. McAndrew (22683641) (author)
अन्य लेखक: Jonathan Cook (3435386) (author), Amy Gill (14910614) (author), Kavya Sahoo (22683644) (author), Clare Thomas (7943747) (author), Phillip J. Stansfeld (1267617) (author), Allister Crow (263807) (author)
प्रकाशित: 2025
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सारांश:<p><b>(A)</b> Pairwise <i><i>in silico</i></i> co-folding of YbbP with all sec/tat secreted proteins from <i><i>Escherichia coli</i></i>. Each co-fold attempt is represented on the 2D plot by their pDockQ score and Molprobity clash score. The TesA-YbbP pair is indicated. <b>(B)</b> Genomic arrangement of YbbA, YbbP, TesA, and YbbO genes in <i><i>E. coli</i></i>. <b>(C)</b> Coevolution analysis between YbbP and TesA. All inter-protein residues were scored for coevolution using CCMPred, converted to a Z-score, and depicted as a frequency histogram. Coevolving residue pairs between YbbP and TesA with Z-scores above 10 are annotated (Further coevolving residue pairs within the YbbAP-TesA complex are given in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003427#pbio.3003427.s001" target="_blank">S1 Table</a>). <b>(D)</b> SDS-PAGE gel showing co-purification of YbbAP-TesA as a complex. Underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003427#pbio.3003427.s018" target="_blank">S1 Data</a>–<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003427#pbio.3003427.s020" target="_blank">S3 Data</a>.</p>