Deep SIM imaging of VGluT1 and VGluT2 nanoclusters and dendritic spines in expanded brain cryosections.

<p>3D rendering of a 166 × 166 × 40 µm brain section from Thy1-YFP-H mice, initially sectioned at 5 µm and expanded isotropically by a factor of eight using the Magnify approach. YFP (gray) labels spine morphology, VGluT1 (magenta) identifies CC synapses, and VGluT2 (cyan) marks TC synapses....

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Bibliographic Details
Main Author:	Yeasmin Akter (7378415) (author)
Other Authors:	Grace Jones (1840372) (author), Grant J. Daskivich (21001625) (author), Victoria Shifflett (21001628) (author), Karina J. Vargas (21001631) (author), Martin Hruska (355590) (author)
Published:	2025
Subjects:	Biophysics Biochemistry Medicine Cell Biology Neuroscience Physiology Biotechnology Evolutionary Biology Developmental Biology Infectious Diseases Biological Sciences not elsewhere classified domain camelid nanobodies combining nanobody labeling single aligned nanomodule organization using tau multiple aligned psd complex brain tissue architecture remains elusive receiving cc input tc synapses varied characterized single afferent input synapses along diverse synapses cc synapses xlink "> tc spines tc afferents synaptic function synaptic architecture subcellular localization sted nanoscopy spine volume resolution imaging nanoscale diversity molecular logic layers examined largely due findings highlight dendritic location constant size brain organize bassoon nanomodules basal axis
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Deep SIM imaging of VGluT1 and VGluT2 nanoclusters and dendritic spines in expanded brain cryosections.

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