New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway.
<p>Extracted ion chromatograms for compound <b>1</b> (<i>m</i>/<i>z</i> 282.0387, shown in black lines) and compound <b>2</b>/<b>3</b> (<i>m</i>/<i>z</i> 240.0280, shown in red lines) in the enzymatic reaction...
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2025
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| Izvleček: | <p>Extracted ion chromatograms for compound <b>1</b> (<i>m</i>/<i>z</i> 282.0387, shown in black lines) and compound <b>2</b>/<b>3</b> (<i>m</i>/<i>z</i> 240.0280, shown in red lines) in the enzymatic reaction mixture of GlcNAc-6P with NagS and NagA (<b>a</b>) or deactivated NagA <b>(b)</b>. <b>(c)</b> Updated metabolic pathway of amino sugar in <i>Streptomyces</i>. Based on the metabolic pathway in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003514#pbio.3003514.s001" target="_blank">S1 Fig</a>, we propose a new metabolic route in GlcNAc metabolism. Apart from the canonical reaction whereby GlcNAc-6P is metabolized by NagA and NagB to fructose-6P, GlcNAc-6P can also be dehydrated by NagS and subsequently deacetylated by NagA to form compound <b>3</b> (shown in light red), whose is a likely toxic compound whose chemical structure is similar to ribose-5P. <b>(d)</b> Ribose by-passes GlcNAc toxicity. Spores (5 × 10<sup>5</sup> CFU) of the <i>S. coelicolor</i> M145 <i>nagB</i> mutant were spotted on MM supplemented with 1% mannitol plus 10 mM GlcNAc and different concentrations (0–20 mM) of either glucose or D-ribose, followed by incubation for 72 h at 30 °C. Note that 1 mM ribose or more alleviates GlcNAc toxicity and allows the cells to grow, while even at 20 mM glucose the colonies still are sensitive to GlcNAc. Single colonies are most likely suppressors.</p> |
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