New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway.
<p>Extracted ion chromatograms for compound <b>1</b> (<i>m</i>/<i>z</i> 282.0387, shown in black lines) and compound <b>2</b>/<b>3</b> (<i>m</i>/<i>z</i> 240.0280, shown in red lines) in the enzymatic reaction...
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2025
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| _version_ | 1849927625784426496 |
|---|---|
| author | Chao Li (145513) |
| author2 | Mia Urem (22683580) Ioli Kotsogianni (9930187) Josephine Lau (20442343) Chao Du (288036) Somayah S. Elsayed (9192571) Nathaniel I. Martin (847378) Iain W. McNae (840777) Patrick Voskamp (2311429) Christoph Mayer (57204) Sébastien Rigali (724082) Navraj Pannu (10163763) Jan Pieter Abrahams (1429531) Lennart Schada von Borzyskowski (22683583) Gilles P. van Wezel (7838948) |
| author2_role | author author author author author author author author author author author author author author |
| author_facet | Chao Li (145513) Mia Urem (22683580) Ioli Kotsogianni (9930187) Josephine Lau (20442343) Chao Du (288036) Somayah S. Elsayed (9192571) Nathaniel I. Martin (847378) Iain W. McNae (840777) Patrick Voskamp (2311429) Christoph Mayer (57204) Sébastien Rigali (724082) Navraj Pannu (10163763) Jan Pieter Abrahams (1429531) Lennart Schada von Borzyskowski (22683583) Gilles P. van Wezel (7838948) |
| author_role | author |
| dc.creator.none.fl_str_mv | Chao Li (145513) Mia Urem (22683580) Ioli Kotsogianni (9930187) Josephine Lau (20442343) Chao Du (288036) Somayah S. Elsayed (9192571) Nathaniel I. Martin (847378) Iain W. McNae (840777) Patrick Voskamp (2311429) Christoph Mayer (57204) Sébastien Rigali (724082) Navraj Pannu (10163763) Jan Pieter Abrahams (1429531) Lennart Schada von Borzyskowski (22683583) Gilles P. van Wezel (7838948) |
| dc.date.none.fl_str_mv | 2025-11-25T19:00:37Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003514.g005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/New_function_of_i_S_coelicolor_i_NagA_and_an_updated_amino_sugar_metabolic_pathway_/30715211 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Microbiology Ecology Developmental Biology Inorganic Chemistry Biological Sciences not elsewhere classified controls nutrient signaling also revealed 6 highlighted key residues glcnac sensing requires toxicity pathway dependent 6p deacetylase naga novel glcnac 6 key role substrate glcnac novel enzyme work uncovers wall leads unprecedented reaction substrate mycelium structural analogue streptomycetaceae </ streptomyces </ revolves around promiscuous activity nature ’ n </ multicellular lifestyle metabolic checkpoint medicine makers lytic dismantling landmark event hyphal cell highly conserved growth media function analysis clinical antibiotics central metabolism catalytic inhibitor antibiotic production active site >- acetylglucosamine 6p dehydratase |
| dc.title.none.fl_str_mv | New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Extracted ion chromatograms for compound <b>1</b> (<i>m</i>/<i>z</i> 282.0387, shown in black lines) and compound <b>2</b>/<b>3</b> (<i>m</i>/<i>z</i> 240.0280, shown in red lines) in the enzymatic reaction mixture of GlcNAc-6P with NagS and NagA (<b>a</b>) or deactivated NagA <b>(b)</b>. <b>(c)</b> Updated metabolic pathway of amino sugar in <i>Streptomyces</i>. Based on the metabolic pathway in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003514#pbio.3003514.s001" target="_blank">S1 Fig</a>, we propose a new metabolic route in GlcNAc metabolism. Apart from the canonical reaction whereby GlcNAc-6P is metabolized by NagA and NagB to fructose-6P, GlcNAc-6P can also be dehydrated by NagS and subsequently deacetylated by NagA to form compound <b>3</b> (shown in light red), whose is a likely toxic compound whose chemical structure is similar to ribose-5P. <b>(d)</b> Ribose by-passes GlcNAc toxicity. Spores (5 × 10<sup>5</sup> CFU) of the <i>S. coelicolor</i> M145 <i>nagB</i> mutant were spotted on MM supplemented with 1% mannitol plus 10 mM GlcNAc and different concentrations (0–20 mM) of either glucose or D-ribose, followed by incubation for 72 h at 30 °C. Note that 1 mM ribose or more alleviates GlcNAc toxicity and allows the cells to grow, while even at 20 mM glucose the colonies still are sensitive to GlcNAc. Single colonies are most likely suppressors.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_103f903401adca61e6f2d3611f3a9f93 |
| identifier_str_mv | 10.1371/journal.pbio.3003514.g005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30715211 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway.Chao Li (145513)Mia Urem (22683580)Ioli Kotsogianni (9930187)Josephine Lau (20442343)Chao Du (288036)Somayah S. Elsayed (9192571)Nathaniel I. Martin (847378)Iain W. McNae (840777)Patrick Voskamp (2311429)Christoph Mayer (57204)Sébastien Rigali (724082)Navraj Pannu (10163763)Jan Pieter Abrahams (1429531)Lennart Schada von Borzyskowski (22683583)Gilles P. van Wezel (7838948)BiochemistryMicrobiologyEcologyDevelopmental BiologyInorganic ChemistryBiological Sciences not elsewhere classifiedcontrols nutrient signalingalso revealed 6highlighted key residuesglcnac sensing requirestoxicity pathway dependent6p deacetylase naganovel glcnac 6key rolesubstrate glcnacnovel enzymework uncoverswall leadsunprecedented reactionsubstrate myceliumstructural analoguestreptomycetaceae </streptomyces </revolves aroundpromiscuous activitynature ’n </multicellular lifestylemetabolic checkpointmedicine makerslytic dismantlinglandmark eventhyphal cellhighly conservedgrowth mediafunction analysisclinical antibioticscentral metabolismcatalytic inhibitorantibiotic productionactive site>- acetylglucosamine6p dehydratase<p>Extracted ion chromatograms for compound <b>1</b> (<i>m</i>/<i>z</i> 282.0387, shown in black lines) and compound <b>2</b>/<b>3</b> (<i>m</i>/<i>z</i> 240.0280, shown in red lines) in the enzymatic reaction mixture of GlcNAc-6P with NagS and NagA (<b>a</b>) or deactivated NagA <b>(b)</b>. <b>(c)</b> Updated metabolic pathway of amino sugar in <i>Streptomyces</i>. Based on the metabolic pathway in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003514#pbio.3003514.s001" target="_blank">S1 Fig</a>, we propose a new metabolic route in GlcNAc metabolism. Apart from the canonical reaction whereby GlcNAc-6P is metabolized by NagA and NagB to fructose-6P, GlcNAc-6P can also be dehydrated by NagS and subsequently deacetylated by NagA to form compound <b>3</b> (shown in light red), whose is a likely toxic compound whose chemical structure is similar to ribose-5P. <b>(d)</b> Ribose by-passes GlcNAc toxicity. Spores (5 × 10<sup>5</sup> CFU) of the <i>S. coelicolor</i> M145 <i>nagB</i> mutant were spotted on MM supplemented with 1% mannitol plus 10 mM GlcNAc and different concentrations (0–20 mM) of either glucose or D-ribose, followed by incubation for 72 h at 30 °C. Note that 1 mM ribose or more alleviates GlcNAc toxicity and allows the cells to grow, while even at 20 mM glucose the colonies still are sensitive to GlcNAc. Single colonies are most likely suppressors.</p>2025-11-25T19:00:37ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003514.g005https://figshare.com/articles/figure/New_function_of_i_S_coelicolor_i_NagA_and_an_updated_amino_sugar_metabolic_pathway_/30715211CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307152112025-11-25T19:00:37Z |
| spellingShingle | New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway. Chao Li (145513) Biochemistry Microbiology Ecology Developmental Biology Inorganic Chemistry Biological Sciences not elsewhere classified controls nutrient signaling also revealed 6 highlighted key residues glcnac sensing requires toxicity pathway dependent 6p deacetylase naga novel glcnac 6 key role substrate glcnac novel enzyme work uncovers wall leads unprecedented reaction substrate mycelium structural analogue streptomycetaceae </ streptomyces </ revolves around promiscuous activity nature ’ n </ multicellular lifestyle metabolic checkpoint medicine makers lytic dismantling landmark event hyphal cell highly conserved growth media function analysis clinical antibiotics central metabolism catalytic inhibitor antibiotic production active site >- acetylglucosamine 6p dehydratase |
| status_str | publishedVersion |
| title | New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway. |
| title_full | New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway. |
| title_fullStr | New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway. |
| title_full_unstemmed | New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway. |
| title_short | New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway. |
| title_sort | New function of <i>S. coelicolor</i> NagA and an updated amino sugar metabolic pathway. |
| topic | Biochemistry Microbiology Ecology Developmental Biology Inorganic Chemistry Biological Sciences not elsewhere classified controls nutrient signaling also revealed 6 highlighted key residues glcnac sensing requires toxicity pathway dependent 6p deacetylase naga novel glcnac 6 key role substrate glcnac novel enzyme work uncovers wall leads unprecedented reaction substrate mycelium structural analogue streptomycetaceae </ streptomyces </ revolves around promiscuous activity nature ’ n </ multicellular lifestyle metabolic checkpoint medicine makers lytic dismantling landmark event hyphal cell highly conserved growth media function analysis clinical antibiotics central metabolism catalytic inhibitor antibiotic production active site >- acetylglucosamine 6p dehydratase |