3D reconstruction of confocal-resolved VGluT1 and VGluT2 clusters and corresponding YFP-labeled apical dendrites.

<p>Representative 23 × 23 × 2.8 µm reconstructions of confocal channels in apical segments of L5 pyramidal neurons generated by 3D-rendering of 17 optical sections spaced at 170 nm from five-channel confocal/3D-tau-STED imaging of apical dendrites and dendritic spines on L5 pyramidal neurons i...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Yeasmin Akter (7378415) (author)
مؤلفون آخرون:	Grace Jones (1840372) (author), Grant J. Daskivich (21001625) (author), Victoria Shifflett (21001628) (author), Karina J. Vargas (21001631) (author), Martin Hruska (355590) (author)
منشور في:	2025
الموضوعات:	Biophysics Biochemistry Medicine Cell Biology Neuroscience Physiology Biotechnology Evolutionary Biology Developmental Biology Infectious Diseases Biological Sciences not elsewhere classified domain camelid nanobodies combining nanobody labeling single aligned nanomodule organization using tau multiple aligned psd complex brain tissue architecture remains elusive receiving cc input tc synapses varied characterized single afferent input synapses along diverse synapses cc synapses xlink "> tc spines tc afferents synaptic function synaptic architecture subcellular localization sted nanoscopy spine volume resolution imaging nanoscale diversity molecular logic layers examined largely due findings highlight dendritic location constant size brain organize bassoon nanomodules basal axis
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الملخص:	<p>Representative 23 × 23 × 2.8 µm reconstructions of confocal channels in apical segments of L5 pyramidal neurons generated by 3D-rendering of 17 optical sections spaced at 170 nm from five-channel confocal/3D-tau-STED imaging of apical dendrites and dendritic spines on L5 pyramidal neurons in S1 of Thy-1-YFP-H mice. YFP-labeled dendrites and spines (white, enhanced by the labeling with GFP antibody), VGluT1 (red, nanobody/Atto 542), and VGluT2 (green, antibody/Alexa Flour 750) were imaged in confocal resolution. Dendrites and clusters were reconstructed using Neurolucida-360 to assign CC and TC synapses to YFP-labeled spines based on their colocalization with VGluT1 or VGluT2, respectively. Still, images of this apical dendrite and spines are shown in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002649#pbio.3002649.g005" target="_blank">Fig 5</a>.</p> <p>(MP4)</p>

3D reconstruction of confocal-resolved VGluT1 and VGluT2 clusters and corresponding YFP-labeled apical dendrites.

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