Table 1_Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease.docx
Background<p>Polygala tenuifolia Willd (PT) is a plant with both medicinal and edible values. Traditionally, it has been used for sedation, enhancing cognition, resolving phlegm, and relieving cough. However, its protective effects and mechanisms against chronic obstructive pulmonary disease (...
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2025
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| _version_ | 1849927636608876544 |
|---|---|
| author | Yiming An (2746240) |
| author2 | Xiao Yu (139738) Chao Wang (146527) Xin Yu (124814) Jingtong Zheng (128345) Hongqiang Lin (14764759) |
| author2_role | author author author author author |
| author_facet | Yiming An (2746240) Xiao Yu (139738) Chao Wang (146527) Xin Yu (124814) Jingtong Zheng (128345) Hongqiang Lin (14764759) |
| author_role | author |
| dc.creator.none.fl_str_mv | Yiming An (2746240) Xiao Yu (139738) Chao Wang (146527) Xin Yu (124814) Jingtong Zheng (128345) Hongqiang Lin (14764759) |
| dc.date.none.fl_str_mv | 2025-11-25T06:15:31Z |
| dc.identifier.none.fl_str_mv | 10.3389/fmicb.2025.1703853.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Table_1_Integrative_network_pharmacology_transcriptomics_and_microbiomics_elucidate_the_therapeutic_mechanism_of_Polygala_tenuifolia_Willd_water_extract_in_chronic_obstructive_pulmonary_disease_docx/30703673 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Microbiology Polygala tenuifolia Willd water extract chronic obstructive pulmonary disease transcriptomics network pharmacology gut microbiota |
| dc.title.none.fl_str_mv | Table 1_Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease.docx |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | Background<p>Polygala tenuifolia Willd (PT) is a plant with both medicinal and edible values. Traditionally, it has been used for sedation, enhancing cognition, resolving phlegm, and relieving cough. However, its protective effects and mechanisms against chronic obstructive pulmonary disease (COPD) remain unclear.</p>Aim of the study<p>This study aims to observe the protective effects of the water extract of Polygala tenuifolia Willd (WEPT) on COPD, and to preliminarily elucidate its potential therapeutic mechanisms by integrating network pharmacology, molecular docking, multi-omics analysis, and molecular experiments.</p>Methods and materials<p>HPLC quantified WEPT constituents. COPD mice models established via chronic smoke exposure underwent WEPT treatment, and the therapeutic effect was evaluated by lung function test, histopathology and cytokine profiling. Integrated multi-omics analyses (network pharmacology, transcriptomics, microbiomics) identified bioactive compounds, therapeutic targets, pathway regulations, and microbiota dynamics. Molecular docking validated compound-target interactions, while immunohistochemical/fluorescence assays confirmed key protein expression in lung tissues.</p>Results<p>WEPT administration effectively reduced inflammatory cytokine levels in COPD mice, improved lung function, and alleviated histopathological damage like alveolar structural injury and airway inflammation. Network pharmacology and transcriptomic analyses identified Norhyoscyamine and Onjixanthone I as key active components, targeting PIK3CA and AKT1 via PI3K-AKT pathway regulation. Microbiome analysis showed WEPT restored gut microbiota balance. Molecular docking confirmed strong binding of bioactive compounds to core targets, while immunostaining assays demonstrated WEPT suppressed p-PI3K and p-AKT protein expression.</p>Conclusion<p>WEPT may exert its intervention effects on COPD through a multi-target and multi-level comprehensive regulatory mechanism.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_12c243bcac1cfc47819a975913d505fe |
| identifier_str_mv | 10.3389/fmicb.2025.1703853.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30703673 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Table 1_Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease.docxYiming An (2746240)Xiao Yu (139738)Chao Wang (146527)Xin Yu (124814)Jingtong Zheng (128345)Hongqiang Lin (14764759)MicrobiologyPolygala tenuifolia Willdwater extractchronic obstructive pulmonary diseasetranscriptomicsnetwork pharmacologygut microbiotaBackground<p>Polygala tenuifolia Willd (PT) is a plant with both medicinal and edible values. Traditionally, it has been used for sedation, enhancing cognition, resolving phlegm, and relieving cough. However, its protective effects and mechanisms against chronic obstructive pulmonary disease (COPD) remain unclear.</p>Aim of the study<p>This study aims to observe the protective effects of the water extract of Polygala tenuifolia Willd (WEPT) on COPD, and to preliminarily elucidate its potential therapeutic mechanisms by integrating network pharmacology, molecular docking, multi-omics analysis, and molecular experiments.</p>Methods and materials<p>HPLC quantified WEPT constituents. COPD mice models established via chronic smoke exposure underwent WEPT treatment, and the therapeutic effect was evaluated by lung function test, histopathology and cytokine profiling. Integrated multi-omics analyses (network pharmacology, transcriptomics, microbiomics) identified bioactive compounds, therapeutic targets, pathway regulations, and microbiota dynamics. Molecular docking validated compound-target interactions, while immunohistochemical/fluorescence assays confirmed key protein expression in lung tissues.</p>Results<p>WEPT administration effectively reduced inflammatory cytokine levels in COPD mice, improved lung function, and alleviated histopathological damage like alveolar structural injury and airway inflammation. Network pharmacology and transcriptomic analyses identified Norhyoscyamine and Onjixanthone I as key active components, targeting PIK3CA and AKT1 via PI3K-AKT pathway regulation. Microbiome analysis showed WEPT restored gut microbiota balance. Molecular docking confirmed strong binding of bioactive compounds to core targets, while immunostaining assays demonstrated WEPT suppressed p-PI3K and p-AKT protein expression.</p>Conclusion<p>WEPT may exert its intervention effects on COPD through a multi-target and multi-level comprehensive regulatory mechanism.</p>2025-11-25T06:15:31ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fmicb.2025.1703853.s001https://figshare.com/articles/dataset/Table_1_Integrative_network_pharmacology_transcriptomics_and_microbiomics_elucidate_the_therapeutic_mechanism_of_Polygala_tenuifolia_Willd_water_extract_in_chronic_obstructive_pulmonary_disease_docx/30703673CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307036732025-11-25T06:15:31Z |
| spellingShingle | Table 1_Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease.docx Yiming An (2746240) Microbiology Polygala tenuifolia Willd water extract chronic obstructive pulmonary disease transcriptomics network pharmacology gut microbiota |
| status_str | publishedVersion |
| title | Table 1_Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease.docx |
| title_full | Table 1_Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease.docx |
| title_fullStr | Table 1_Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease.docx |
| title_full_unstemmed | Table 1_Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease.docx |
| title_short | Table 1_Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease.docx |
| title_sort | Table 1_Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism of Polygala tenuifolia Willd water extract in chronic obstructive pulmonary disease.docx |
| topic | Microbiology Polygala tenuifolia Willd water extract chronic obstructive pulmonary disease transcriptomics network pharmacology gut microbiota |