Membrane integrity in the <i>yqhG</i> mutant.

Membrane integrity in the <i>yqhG</i> mutant.

<p>Fluorescence-based quantification of membrane permeability in wild-type (WT), Δ<i>yqhG</i>, and complemented (Δ<i>yqhG</i>-C) strains using SYTOX Green uptake. Bacteria were incubated with 1 μM SYTOX Green, and fluorescence intensity (RFU) was measured after 30 min....

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Autore principale: Jianhua Liao (1393228) (author)
Altri autori: Jun Cheng (194158) (author), Baoqing Liu (1508821) (author), Yuzhi Shao (22683349) (author), Chunyan Meng (5993228) (author)
Pubblicazione: 2025
Soggetti:
Medicine
Microbiology
Cell Biology
Biotechnology
Evolutionary Biology
Ecology
Science Policy
Infectious Diseases
Virology
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
therapeutic strategies aimed
novel therapeutic approaches
murine infection model
div >< p
defense mechanisms employed
conserved gene encoding
compromising membrane stability
affect biofilm formation
staphylococcus aureus </
reduced bacterial burden
improved host survival
maintaining mrsa ’
oxidative stress resistance
yqhg </
withstand host
reduced motility
oxidative stress
increasing resistance
stress adaptation
potential target
periplasmic protein
mrsa virulence
mrsa pathogenesis
key determinant
infected organs
increased sensitivity
imposed stresses
growing prevalence
findings highlight
existing antibiotics
critical need
attenuates virulence
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Riassunto:<p>Fluorescence-based quantification of membrane permeability in wild-type (WT), Δ<i>yqhG</i>, and complemented (Δ<i>yqhG</i>-C) strains using SYTOX Green uptake. Bacteria were incubated with 1 μM SYTOX Green, and fluorescence intensity (RFU) was measured after 30 min. Bars represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA. *<i>P</i> < 0.05.</p>

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