Membrane integrity in the <i>yqhG</i> mutant.
<p>Fluorescence-based quantification of membrane permeability in wild-type (WT), Δ<i>yqhG</i>, and complemented (Δ<i>yqhG</i>-C) strains using SYTOX Green uptake. Bacteria were incubated with 1 μM SYTOX Green, and fluorescence intensity (RFU) was measured after 30 min....
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| מחברים אחרים: | , , , |
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2025
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| _version_ | 1849927628600901632 |
|---|---|
| author | Jianhua Liao (1393228) |
| author2 | Jun Cheng (194158) Baoqing Liu (1508821) Yuzhi Shao (22683349) Chunyan Meng (5993228) |
| author2_role | author author author author |
| author_facet | Jianhua Liao (1393228) Jun Cheng (194158) Baoqing Liu (1508821) Yuzhi Shao (22683349) Chunyan Meng (5993228) |
| author_role | author |
| dc.creator.none.fl_str_mv | Jianhua Liao (1393228) Jun Cheng (194158) Baoqing Liu (1508821) Yuzhi Shao (22683349) Chunyan Meng (5993228) |
| dc.date.none.fl_str_mv | 2025-11-25T18:28:05Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0337292.g003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Membrane_integrity_in_the_i_yqhG_i_mutant_/30713755 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence |
| dc.title.none.fl_str_mv | Membrane integrity in the <i>yqhG</i> mutant. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Fluorescence-based quantification of membrane permeability in wild-type (WT), Δ<i>yqhG</i>, and complemented (Δ<i>yqhG</i>-C) strains using SYTOX Green uptake. Bacteria were incubated with 1 μM SYTOX Green, and fluorescence intensity (RFU) was measured after 30 min. Bars represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA. *<i>P</i> < 0.05.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_13b360bf0a6ee0dffabc54aea58b0a1b |
| identifier_str_mv | 10.1371/journal.pone.0337292.g003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30713755 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Membrane integrity in the <i>yqhG</i> mutant.Jianhua Liao (1393228)Jun Cheng (194158)Baoqing Liu (1508821)Yuzhi Shao (22683349)Chunyan Meng (5993228)MedicineMicrobiologyCell BiologyBiotechnologyEvolutionary BiologyEcologyScience PolicyInfectious DiseasesVirologyEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedtherapeutic strategies aimednovel therapeutic approachesmurine infection modeldiv >< pdefense mechanisms employedconserved gene encodingcompromising membrane stabilityaffect biofilm formationstaphylococcus aureus </reduced bacterial burdenimproved host survivalmaintaining mrsa ’oxidative stress resistanceyqhg </withstand hostreduced motilityoxidative stressincreasing resistancestress adaptationpotential targetperiplasmic proteinmrsa virulencemrsa pathogenesiskey determinantinfected organsincreased sensitivityimposed stressesgrowing prevalencefindings highlightexisting antibioticscritical needattenuates virulence<p>Fluorescence-based quantification of membrane permeability in wild-type (WT), Δ<i>yqhG</i>, and complemented (Δ<i>yqhG</i>-C) strains using SYTOX Green uptake. Bacteria were incubated with 1 μM SYTOX Green, and fluorescence intensity (RFU) was measured after 30 min. Bars represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA. *<i>P</i> < 0.05.</p>2025-11-25T18:28:05ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pone.0337292.g003https://figshare.com/articles/figure/Membrane_integrity_in_the_i_yqhG_i_mutant_/30713755CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307137552025-11-25T18:28:05Z |
| spellingShingle | Membrane integrity in the <i>yqhG</i> mutant. Jianhua Liao (1393228) Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence |
| status_str | publishedVersion |
| title | Membrane integrity in the <i>yqhG</i> mutant. |
| title_full | Membrane integrity in the <i>yqhG</i> mutant. |
| title_fullStr | Membrane integrity in the <i>yqhG</i> mutant. |
| title_full_unstemmed | Membrane integrity in the <i>yqhG</i> mutant. |
| title_short | Membrane integrity in the <i>yqhG</i> mutant. |
| title_sort | Membrane integrity in the <i>yqhG</i> mutant. |
| topic | Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence |