Membrane integrity in the <i>yqhG</i> mutant.

Membrane integrity in the <i>yqhG</i> mutant.

<p>Fluorescence-based quantification of membrane permeability in wild-type (WT), Δ<i>yqhG</i>, and complemented (Δ<i>yqhG</i>-C) strains using SYTOX Green uptake. Bacteria were incubated with 1 μM SYTOX Green, and fluorescence intensity (RFU) was measured after 30 min....

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Bibliografiske detaljer
Hovedforfatter:	Jianhua Liao (1393228) (author)
Andre forfattere:	Jun Cheng (194158) (author), Baoqing Liu (1508821) (author), Yuzhi Shao (22683349) (author), Chunyan Meng (5993228) (author)
Udgivet:	2025
Fag:	Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence
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Lignende værker

Surface motility of the <i>yqhG</i> mutant.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Oxidative stress resistance of the <i>yqhG</i> mutant.
af: Jianhua Liao (1393228)
Udgivet: (2025)

YqhG is required for systemic virulence but not for <i>in vitro</i> growth.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Kidney bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Heart bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Optical density measurements of MRSA wild-type, Δ<i>yqhG</i>, and complemented strains during <i>in vitro</i> growth.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Survival data of BALB/c mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Crystal violet quantification of biofilm formation in MRSA wild-type, ΔyqhG, and complemented strains.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Spleen bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Liver bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Amount of PSMs (μg/1mL culture) in MRSA wild-type, ΔyqhG, and complemented strains.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Primers Used in This Study.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Bacterial Strains and Plasmids Used in This Study.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Biofilm formation in MRSA strains.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Viable CFU counts following hydrogen peroxide exposure.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Colony diameter measurements from soft agar motility assays.
af: Jianhua Liao (1393228)
Udgivet: (2025)

SYTOX Green fluorescence measurements for membrane permeability assessment.
af: Jianhua Liao (1393228)
Udgivet: (2025)

Table 1_Hard to jump: host shifts appear unlikely in a T4-like phage evolved in the lab.xlsx
af: Yu Ning (588573)
Udgivet: (2025)

Supplementary file 1_Hard to jump: host shifts appear unlikely in a T4-like phage evolved in the lab.docx
af: Yu Ning (588573)
Udgivet: (2025)

Protocatechuic Acid alleviates D-gal-induced renal senescence and injury in mice by regulating taurine metabolism
af: Songhao Tian (22609826)
Udgivet: (2025)

Table 1_Melatonin mitigates cadmium-induced oxidative damage and modulates polysaccharide biosynthesis in Bletilla striata.xlsx
af: Deqiong Niu (19691873)
Udgivet: (2025)

Data Sheet 1_Melatonin mitigates cadmium-induced oxidative damage and modulates polysaccharide biosynthesis in Bletilla striata.docx
af: Deqiong Niu (19691873)
Udgivet: (2025)

Potent phenolics with antimigratory and anti-MRSA powers from fungus associated with <i>Saussurea involucrata</i>
af: Wei-hua Yuan (22683169)
Udgivet: (2025)

Effects of <i>T. gondii</i> infection on rat gut microbiota diversity.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Untargeted serum metabolomics analysis before and after <i>T. gondii</i> infection.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Functional analysis of the intestinal microbial community following <i>T. gondii</i> infection.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Composition of intestinal microbial communities in the large intestine of rats before and after <i>T. gondii</i> infection.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Composition of intestinal microbial communities in the small intestine of rats before and after <i>T. gondii</i> infection.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Differential metabolite results, listing metabolites with significant changes across treatment groups, including fold changes, and <i>p</i>-values.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Correlation network between intestinal bacterial species and differential serum metabolites.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

KEGG pathway enrichment analysis of differential serum metabolites.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Spearman correlation analysis between gut microbial taxa and circulating metabolites, detailing correlation coefficients, significance values, and taxon–metabolite pairings indicative of microbiome–host metabolic interactions.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Pathway enrichment analysis of differential metabolites, highlighting significantly impacted metabolic pathways and associated enrichment statistics based on KEGG or other pathway databases.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Orthogonal partial least squares-discriminant analysis (OPLS-DA) of serum metabolomics data.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Quality control assessment of serum metabolomics data.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Heatmap of enriched KEGG pathways across samples, illustrating variations in pathway abundance.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

Graphical abstract.
af: Ji-Xin Zhao (22056386)
Udgivet: (2025)

The U sequence method.
af: Pierre Angelvy (22683450)
Udgivet: (2025)

Provisional study schedule of MUSIBOT.
af: Pierre Angelvy (22683450)
Udgivet: (2025)

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