Membrane integrity in the <i>yqhG</i> mutant.

Membrane integrity in the <i>yqhG</i> mutant.

<p>Fluorescence-based quantification of membrane permeability in wild-type (WT), Δ<i>yqhG</i>, and complemented (Δ<i>yqhG</i>-C) strains using SYTOX Green uptake. Bacteria were incubated with 1 μM SYTOX Green, and fluorescence intensity (RFU) was measured after 30 min....

पूर्ण विवरण

में बचाया:

ग्रंथसूची विवरण
मुख्य लेखक:	Jianhua Liao (1393228) (author)
अन्य लेखक:	Jun Cheng (194158) (author), Baoqing Liu (1508821) (author), Yuzhi Shao (22683349) (author), Chunyan Meng (5993228) (author)
प्रकाशित:	2025
विषय:	Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence
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समान संसाधन

Surface motility of the <i>yqhG</i> mutant.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Oxidative stress resistance of the <i>yqhG</i> mutant.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

YqhG is required for systemic virulence but not for <i>in vitro</i> growth.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Kidney bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Heart bacterial burden in mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Optical density measurements of MRSA wild-type, Δ<i>yqhG</i>, and complemented strains during <i>in vitro</i> growth.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Survival data of BALB/c mice infected with MRSA wild-type, Δ<i>yqhG</i>, and complemented strains.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Crystal violet quantification of biofilm formation in MRSA wild-type, ΔyqhG, and complemented strains.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Spleen bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Liver bacterial burden in mice infected with MRSA wild-type, ΔyqhG, and complemented strains.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Amount of PSMs (μg/1mL culture) in MRSA wild-type, ΔyqhG, and complemented strains.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Quantification of cell-associated PSMs in wild-type, Δ<i>yqhG</i>, and complemented strains. Cell-associated PSMs were extracted from bacterial pellets using 6 M guanidine hydrochloride and quantified by reversed-phase HPLC. The Δ<i>yqhG</i> mutant exhibited a significant reduction in surface-bound PSMs compared with the wild-type strain, whereas complementation restored PSM levels to near wild-type values. Data represent mean ± s.e.m. from three independent experiments. Statistical analysis was performed using one-way ANOVA; *<i>P</i> < 0.05, ns, not significant.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Primers Used in This Study.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Bacterial Strains and Plasmids Used in This Study.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Biofilm formation in MRSA strains.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Viable CFU counts following hydrogen peroxide exposure.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Colony diameter measurements from soft agar motility assays.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

SYTOX Green fluorescence measurements for membrane permeability assessment.
द्वारा: Jianhua Liao (1393228)
प्रकाशित: (2025)

Table 1_Hard to jump: host shifts appear unlikely in a T4-like phage evolved in the lab.xlsx
द्वारा: Yu Ning (588573)
प्रकाशित: (2025)

Supplementary file 1_Hard to jump: host shifts appear unlikely in a T4-like phage evolved in the lab.docx
द्वारा: Yu Ning (588573)
प्रकाशित: (2025)

Protocatechuic Acid alleviates D-gal-induced renal senescence and injury in mice by regulating taurine metabolism
द्वारा: Songhao Tian (22609826)
प्रकाशित: (2025)

Table 1_Melatonin mitigates cadmium-induced oxidative damage and modulates polysaccharide biosynthesis in Bletilla striata.xlsx
द्वारा: Deqiong Niu (19691873)
प्रकाशित: (2025)

Data Sheet 1_Melatonin mitigates cadmium-induced oxidative damage and modulates polysaccharide biosynthesis in Bletilla striata.docx
द्वारा: Deqiong Niu (19691873)
प्रकाशित: (2025)

Potent phenolics with antimigratory and anti-MRSA powers from fungus associated with <i>Saussurea involucrata</i>
द्वारा: Wei-hua Yuan (22683169)
प्रकाशित: (2025)

Effects of <i>T. gondii</i> infection on rat gut microbiota diversity.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Untargeted serum metabolomics analysis before and after <i>T. gondii</i> infection.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Functional analysis of the intestinal microbial community following <i>T. gondii</i> infection.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Composition of intestinal microbial communities in the large intestine of rats before and after <i>T. gondii</i> infection.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Composition of intestinal microbial communities in the small intestine of rats before and after <i>T. gondii</i> infection.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Differential metabolite results, listing metabolites with significant changes across treatment groups, including fold changes, and <i>p</i>-values.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Correlation network between intestinal bacterial species and differential serum metabolites.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

KEGG pathway enrichment analysis of differential serum metabolites.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Spearman correlation analysis between gut microbial taxa and circulating metabolites, detailing correlation coefficients, significance values, and taxon–metabolite pairings indicative of microbiome–host metabolic interactions.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Pathway enrichment analysis of differential metabolites, highlighting significantly impacted metabolic pathways and associated enrichment statistics based on KEGG or other pathway databases.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Orthogonal partial least squares-discriminant analysis (OPLS-DA) of serum metabolomics data.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Quality control assessment of serum metabolomics data.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Heatmap of enriched KEGG pathways across samples, illustrating variations in pathway abundance.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

Graphical abstract.
द्वारा: Ji-Xin Zhao (22056386)
प्रकाशित: (2025)

The U sequence method.
द्वारा: Pierre Angelvy (22683450)
प्रकाशित: (2025)

Provisional study schedule of MUSIBOT.
द्वारा: Pierre Angelvy (22683450)
प्रकाशित: (2025)

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