Additional file 3 of Identification of SLC22A17 DNA methylation hotspot as a potential biomarker in cutaneous melanoma
Supplementary Material 3: Bisulfite conversion and Sanger sequencing analysis of SLC22A17 in melanoma cell lines. (A-D) MethDNA analysis of CpG hotspots included in the sequences Prom 1, 2, 3, and 4 within the SLC22A17 promoter region. (E) MethDNA analysis of CpG hotspots belonging to the body regio...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , |
| منشور في: |
2024
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| الملخص: | Supplementary Material 3: Bisulfite conversion and Sanger sequencing analysis of SLC22A17 in melanoma cell lines. (A-D) MethDNA analysis of CpG hotspots included in the sequences Prom 1, 2, 3, and 4 within the SLC22A17 promoter region. (E) MethDNA analysis of CpG hotspots belonging to the body region. (F-G) MethDNA levels of CpGs located in the 3’UTR region. Yellow circles indicate the CpG hotspots. Dark, gray, and yellow bars represent the sequenced fragments, the primers used for amplification, and CCGG restriction sites, respectively. The pie charts indicate the methDNA status for each CpG, reporting the percentage of methylation (red) and unmethylation (green). Gray pie charts refer to the undetected CpG methDNA levels. The methDNA percentage of each CG probeset was computed as the ratio between the height of the cytosine (unconverted CpG cytosine – methylated) and thymine (converted CpG cytosine – unmethylated) peaks retrieved from the sequence chromatogram obtained for each target |
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