Function determination and kinetics of NagS.

Function determination and kinetics of NagS.

<p>(<b>a</b>) <sup>1</sup>H NMR spectrum of the enzymatic reaction mixture of GlcNAc-6P with either the active NagS (top) or the heated-inactivated one (bottom). The associated NMR peaks of the reaction product are highlighted in red circles and arrows. (<b>b</...

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Main Author: Chao Li (145513) (author)
Other Authors: Mia Urem (22683580) (author), Ioli Kotsogianni (9930187) (author), Josephine Lau (20442343) (author), Chao Du (288036) (author), Somayah S. Elsayed (9192571) (author), Nathaniel I. Martin (847378) (author), Iain W. McNae (840777) (author), Patrick Voskamp (2311429) (author), Christoph Mayer (57204) (author), Sébastien Rigali (724082) (author), Navraj Pannu (10163763) (author), Jan Pieter Abrahams (1429531) (author), Lennart Schada von Borzyskowski (22683583) (author), Gilles P. van Wezel (7838948) (author)
Published: 2025
Subjects:
Biochemistry
Microbiology
Ecology
Developmental Biology
Inorganic Chemistry
Biological Sciences not elsewhere classified
controls nutrient signaling
also revealed 6
highlighted key residues
glcnac sensing requires
toxicity pathway dependent
6p deacetylase naga
novel glcnac 6
key role
substrate glcnac
novel enzyme
work uncovers
wall leads
unprecedented reaction
substrate mycelium
structural analogue
streptomycetaceae </
streptomyces </
revolves around
promiscuous activity
nature ’
n </
multicellular lifestyle
metabolic checkpoint
medicine makers
lytic dismantling
landmark event
hyphal cell
highly conserved
growth media
function analysis
clinical antibiotics
central metabolism
catalytic inhibitor
antibiotic production
active site
>- acetylglucosamine
6p dehydratase
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Summary:<p>(<b>a</b>) <sup>1</sup>H NMR spectrum of the enzymatic reaction mixture of GlcNAc-6P with either the active NagS (top) or the heated-inactivated one (bottom). The associated NMR peaks of the reaction product are highlighted in red circles and arrows. (<b>b</b>) HRESIMS spectrum of the NagS product, compound <b>1</b>: <i>m</i>/<i>z</i> 282.0387 [M-H]<sup>−</sup> (calculated for C<sub>8</sub>H<sub>13</sub>NO<sub>8</sub>P, 282.0384) <b>(c)</b> <sup>1</sup>H NMR spectrum of the enzymatic reaction mixture of ManNAc-6P with either the active NagS (top) or the heat-inactivated one (bottom). The associated NMR peaks of the reaction product are highlighted in red circles. (<b>d</b>) Reactions catalyzed by NagS. NagS dehydrates both GlcNAc-6P and ManNAc-6P to produce compound <b>1</b>. Michaelis-Menten curves were fitted, and selected curves are shown for NagS with the substrates GlcNAc-6P (<b>e</b>) with <i>K</i><sub>m</sub> value of 0.45 ± 0.03 mM and <i>k</i><sub>cat</sub>/<i>K</i><sub>m</sub> value of 5.48 × 10<sup>4</sup> M<sup>−1</sup>·s<sup>−1</sup>, and ManNAc-6P (<b>f</b>) with <i>K</i><sub>m</sub> value of 0.68 ± 0.05 mM and <i>k</i><sub>cat</sub>/<i>K</i><sub>m</sub> value of 1.32 × 10<sup>3</sup> M<sup>−1</sup>·s<sup>−1</sup>. In <b>e</b> and <b>f</b>, the <i>V</i><sub>0</sub> data were plotted against the substrate concentration, and each assay was performed in triplicate and expressed as a mean ± standard error. The data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003514#pbio.3003514.s022" target="_blank">S1 Data</a>.</p> <p>(TIF)</p>

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