Long-Term Single-Particle Tracking of Membrane Proteins Enabled by Upconversion Alkaline-Earth Nanoprobes
Single-particle tracking (SPT) provides mechanistic insights into individual biomolecules but suffers from the limited photostability of conventional probes. Upconversion SPT (uSPT), employing lanthanide-doped nanoparticles (UCNPs) as probes, enables extended imaging durations; however, its broader...
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2025
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| _version_ | 1852018130589581312 |
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| author | Huan Ling (18150399) |
| author2 | Jinyang Liu (2060833) Fan Ding (302565) Fei Zhao (183673) Song Chen (69646) Wenrui Zhang (1486636) Yunxiang Zhang (1301400) Qian Liu (135614) |
| author2_role | author author author author author author author |
| author_facet | Huan Ling (18150399) Jinyang Liu (2060833) Fan Ding (302565) Fei Zhao (183673) Song Chen (69646) Wenrui Zhang (1486636) Yunxiang Zhang (1301400) Qian Liu (135614) |
| author_role | author |
| dc.creator.none.fl_str_mv | Huan Ling (18150399) Jinyang Liu (2060833) Fan Ding (302565) Fei Zhao (183673) Song Chen (69646) Wenrui Zhang (1486636) Yunxiang Zhang (1301400) Qian Liu (135614) |
| dc.date.none.fl_str_mv | 2025-07-29T12:33:56Z |
| dc.identifier.none.fl_str_mv | 10.1021/acs.nanolett.5c01945.s002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/media/Long-Term_Single-Particle_Tracking_of_Membrane_Proteins_Enabled_by_Upconversion_Alkaline-Earth_Nanoprobes/29665515 |
| dc.rights.none.fl_str_mv | CC BY-NC 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Medicine Cell Biology Biotechnology Space Science Physical Sciences not elsewhere classified provides mechanistic insights membrane proteins enabled engineered ucnps exhibit earth nanoprobes single regulating egfr dynamics term single earth materials upconversion alkaline reversible transitions particle tracking nanoparticle size motional states motional heterogeneity markedly altered limited photostability individual egfr individual biomolecules fold increase findings demonstrate enhanced luminescence doped nanoparticles critical role conventional sodium compact dimensions broader application based counterparts based alkaline |
| dc.title.none.fl_str_mv | Long-Term Single-Particle Tracking of Membrane Proteins Enabled by Upconversion Alkaline-Earth Nanoprobes |
| dc.type.none.fl_str_mv | Dataset Media info:eu-repo/semantics/publishedVersion dataset |
| description | Single-particle tracking (SPT) provides mechanistic insights into individual biomolecules but suffers from the limited photostability of conventional probes. Upconversion SPT (uSPT), employing lanthanide-doped nanoparticles (UCNPs) as probes, enables extended imaging durations; however, its broader application is hindered by the trade-off between nanoparticle size and brightness. To overcome this limitation, we designed ∼10 nm core–shell–shell UCNPs incorporating strontium-based alkaline-earth materials, achieving both compact dimensions and enhanced luminescence. These engineered UCNPs exhibit a 4.6-fold increase in brightness compared to that of conventional sodium-based counterparts. Using these probes, we tracked epidermal growth factor receptor (EGFR) dynamics and identified four distinct motion states (immobile, confined, free, and directed) highlighting the motional heterogeneity and reversible transitions of individual EGFR. Treatment with latrunculin A markedly altered these motional states, underscoring the critical role of the cytoskeleton in regulating EGFR dynamics. Collectively, our findings demonstrate the utility of uSPT for probing membrane protein behavior and dissecting complex cellular processes. |
| eu_rights_str_mv | openAccess |
| id | Manara_1a3edbca3feb65f91e08d5bc30efd53c |
| identifier_str_mv | 10.1021/acs.nanolett.5c01945.s002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/29665515 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY-NC 4.0 |
| spelling | Long-Term Single-Particle Tracking of Membrane Proteins Enabled by Upconversion Alkaline-Earth NanoprobesHuan Ling (18150399)Jinyang Liu (2060833)Fan Ding (302565)Fei Zhao (183673)Song Chen (69646)Wenrui Zhang (1486636)Yunxiang Zhang (1301400)Qian Liu (135614)BiophysicsMedicineCell BiologyBiotechnologySpace SciencePhysical Sciences not elsewhere classifiedprovides mechanistic insightsmembrane proteins enabledengineered ucnps exhibitearth nanoprobes singleregulating egfr dynamicsterm singleearth materialsupconversion alkalinereversible transitionsparticle trackingnanoparticle sizemotional statesmotional heterogeneitymarkedly alteredlimited photostabilityindividual egfrindividual biomoleculesfold increasefindings demonstrateenhanced luminescencedoped nanoparticlescritical roleconventional sodiumcompact dimensionsbroader applicationbased counterpartsbased alkalineSingle-particle tracking (SPT) provides mechanistic insights into individual biomolecules but suffers from the limited photostability of conventional probes. Upconversion SPT (uSPT), employing lanthanide-doped nanoparticles (UCNPs) as probes, enables extended imaging durations; however, its broader application is hindered by the trade-off between nanoparticle size and brightness. To overcome this limitation, we designed ∼10 nm core–shell–shell UCNPs incorporating strontium-based alkaline-earth materials, achieving both compact dimensions and enhanced luminescence. These engineered UCNPs exhibit a 4.6-fold increase in brightness compared to that of conventional sodium-based counterparts. Using these probes, we tracked epidermal growth factor receptor (EGFR) dynamics and identified four distinct motion states (immobile, confined, free, and directed) highlighting the motional heterogeneity and reversible transitions of individual EGFR. Treatment with latrunculin A markedly altered these motional states, underscoring the critical role of the cytoskeleton in regulating EGFR dynamics. Collectively, our findings demonstrate the utility of uSPT for probing membrane protein behavior and dissecting complex cellular processes.2025-07-29T12:33:56ZDatasetMediainfo:eu-repo/semantics/publishedVersiondataset10.1021/acs.nanolett.5c01945.s002https://figshare.com/articles/media/Long-Term_Single-Particle_Tracking_of_Membrane_Proteins_Enabled_by_Upconversion_Alkaline-Earth_Nanoprobes/29665515CC BY-NC 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/296655152025-07-29T12:33:56Z |
| spellingShingle | Long-Term Single-Particle Tracking of Membrane Proteins Enabled by Upconversion Alkaline-Earth Nanoprobes Huan Ling (18150399) Biophysics Medicine Cell Biology Biotechnology Space Science Physical Sciences not elsewhere classified provides mechanistic insights membrane proteins enabled engineered ucnps exhibit earth nanoprobes single regulating egfr dynamics term single earth materials upconversion alkaline reversible transitions particle tracking nanoparticle size motional states motional heterogeneity markedly altered limited photostability individual egfr individual biomolecules fold increase findings demonstrate enhanced luminescence doped nanoparticles critical role conventional sodium compact dimensions broader application based counterparts based alkaline |
| status_str | publishedVersion |
| title | Long-Term Single-Particle Tracking of Membrane Proteins Enabled by Upconversion Alkaline-Earth Nanoprobes |
| title_full | Long-Term Single-Particle Tracking of Membrane Proteins Enabled by Upconversion Alkaline-Earth Nanoprobes |
| title_fullStr | Long-Term Single-Particle Tracking of Membrane Proteins Enabled by Upconversion Alkaline-Earth Nanoprobes |
| title_full_unstemmed | Long-Term Single-Particle Tracking of Membrane Proteins Enabled by Upconversion Alkaline-Earth Nanoprobes |
| title_short | Long-Term Single-Particle Tracking of Membrane Proteins Enabled by Upconversion Alkaline-Earth Nanoprobes |
| title_sort | Long-Term Single-Particle Tracking of Membrane Proteins Enabled by Upconversion Alkaline-Earth Nanoprobes |
| topic | Biophysics Medicine Cell Biology Biotechnology Space Science Physical Sciences not elsewhere classified provides mechanistic insights membrane proteins enabled engineered ucnps exhibit earth nanoprobes single regulating egfr dynamics term single earth materials upconversion alkaline reversible transitions particle tracking nanoparticle size motional states motional heterogeneity markedly altered limited photostability individual egfr individual biomolecules fold increase findings demonstrate enhanced luminescence doped nanoparticles critical role conventional sodium compact dimensions broader application based counterparts based alkaline |