The inhibitory impact of glutathione (GSH) and ascorbic acid (vitamin C) compounds on glucose-6-phosphate dehydrogenase (G6PD) enzyme purified from sheep liver
<p>Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme with many essential biochemical functions. However, in various cancer diseases, increased activity of G6PD causes cancer cells to grow, so G6PD inhibitors have become a significant area of research in cancer treatment.</p> <p>...
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2025
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| 總結: | <p>Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme with many essential biochemical functions. However, in various cancer diseases, increased activity of G6PD causes cancer cells to grow, so G6PD inhibitors have become a significant area of research in cancer treatment.</p> <p>Here, G6PD was purified 4530-fold with affinity chromatography using 2’,5′-ADP Sepharose 4B from sheep liver. The effects of reduced glutathione (GSH) and ascorbic acid (vitamin C) on G6PD activity were explored.</p> <p>GSH and ascorbic acid showed a significant inhibitory effect on G6PD, and IC<sub>50</sub> values were found as 0.37 µM and 34.66 µM, respectively. The inhibition type from Lineweaver-Burk plots of these compounds was identified as non-competitive inhibition. The <i>K<sub>i</sub></i> values of GSH and ascorbic acid were calculated as 0.48 µM and 30.47 µM, respectively.</p> <p>In this study, it was observed that GSH and ascorbic acid antioxidant compounds exhibit an inhibitory effect on G6PD and may be protective and preventive against cancer.</p> |
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