Imaging Electrical Activity of Retinal Ganglion Cells with Fluorescent Voltage and Calcium Indicator Proteins in Retinal Degenerative <i>rd1</i> Blind Mice
In order to understand the retinal network, it is essential to identify functional connectivity among retinal neurons. For this purpose, imaging neuronal activity through fluorescent indicator proteins has been a promising approach, offering simultaneous measurements of neuronal activities from diff...
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2025
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| _version_ | 1849927644240412672 |
|---|---|
| author | Younginha Jung (22676081) |
| author2 | Sungmoo Lee (7554905) Jun Kyu Rhee (11633650) Chae-Eun Lee (14228438) Bradley J. Baker (7554908) Yoon-Kyu Song (6257246) |
| author2_role | author author author author author |
| author_facet | Younginha Jung (22676081) Sungmoo Lee (7554905) Jun Kyu Rhee (11633650) Chae-Eun Lee (14228438) Bradley J. Baker (7554908) Yoon-Kyu Song (6257246) |
| author_role | author |
| dc.creator.none.fl_str_mv | Younginha Jung (22676081) Sungmoo Lee (7554905) Jun Kyu Rhee (11633650) Chae-Eun Lee (14228438) Bradley J. Baker (7554908) Yoon-Kyu Song (6257246) |
| dc.date.none.fl_str_mv | 2025-11-24T17:38:16Z |
| dc.identifier.none.fl_str_mv | 10.1021/acschemneuro.5c00740.s003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/media/Imaging_Electrical_Activity_of_Retinal_Ganglion_Cells_with_Fluorescent_Voltage_and_Calcium_Indicator_Proteins_in_Retinal_Degenerative_i_rd1_i_Blind_Mice/30696122 |
| dc.rights.none.fl_str_mv | CC BY-NC 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Medicine Microbiology Cell Biology Neuroscience Physiology Biotechnology Developmental Biology Chemical Sciences not elsewhere classified potent experimental platform offering simultaneous measurements evoked somatic spiking fluorescent protein sensors fluorescent indicator proteins retinal stimulation strategies retinal signaling pathways retinal ganglion cells calcium indicator proteins calcium concentrations evoked visualize membrane voltage subthreshold voltage changes imaging neuronal activity imaging electrical activity retinal voltage imaging electrical activity fluorescent voltage retinal tissues retinal network retinal degenerative neuronal activities calcium calcium dynamics visual prosthesis show current results indicate rd1 </ promising approach millisecond resolution different regions current pulses cellular precision |
| dc.title.none.fl_str_mv | Imaging Electrical Activity of Retinal Ganglion Cells with Fluorescent Voltage and Calcium Indicator Proteins in Retinal Degenerative <i>rd1</i> Blind Mice |
| dc.type.none.fl_str_mv | Dataset Media info:eu-repo/semantics/publishedVersion dataset |
| description | In order to understand the retinal network, it is essential to identify functional connectivity among retinal neurons. For this purpose, imaging neuronal activity through fluorescent indicator proteins has been a promising approach, offering simultaneous measurements of neuronal activities from different regions of the circuit. In this study, we used genetically encoded indicatorsBongwoori-R3 for voltage or GCaMP6f for calciumto visualize membrane voltage or calcium dynamics, respectively, as spatial maps within individual retinal ganglion cells from retinal tissues of photoreceptor-degenerated <i>rd1</i> mice. Retinal voltage imaging was able to show current-evoked somatic spiking as well as subthreshold voltage changes, while calcium imaging showed changes in calcium concentrations evoked by current pulses in retinal ganglion cells. These results indicate that the combination of fluorescent protein sensors and high-speed imaging methods permits the imaging of electrical activity with cellular precision and millisecond resolution. Hence, we expect our method will provide a potent experimental platform for the study of retinal signaling pathways, as well as the development of retinal stimulation strategies in visual prosthesis. |
| eu_rights_str_mv | openAccess |
| id | Manara_1f49d5c6644378bc399587a4d8718a65 |
| identifier_str_mv | 10.1021/acschemneuro.5c00740.s003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30696122 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY-NC 4.0 |
| spelling | Imaging Electrical Activity of Retinal Ganglion Cells with Fluorescent Voltage and Calcium Indicator Proteins in Retinal Degenerative <i>rd1</i> Blind MiceYounginha Jung (22676081)Sungmoo Lee (7554905)Jun Kyu Rhee (11633650)Chae-Eun Lee (14228438)Bradley J. Baker (7554908)Yoon-Kyu Song (6257246)BiophysicsBiochemistryMedicineMicrobiologyCell BiologyNeurosciencePhysiologyBiotechnologyDevelopmental BiologyChemical Sciences not elsewhere classifiedpotent experimental platformoffering simultaneous measurementsevoked somatic spikingfluorescent protein sensorsfluorescent indicator proteinsretinal stimulation strategiesretinal signaling pathwaysretinal ganglion cellscalcium indicator proteinscalcium concentrations evokedvisualize membrane voltagesubthreshold voltage changesimaging neuronal activityimaging electrical activityretinal voltage imagingelectrical activityfluorescent voltageretinal tissuesretinal networkretinal degenerativeneuronal activitiescalcium calcium dynamicsvisual prosthesisshow currentresults indicaterd1 </promising approachmillisecond resolutiondifferent regionscurrent pulsescellular precisionIn order to understand the retinal network, it is essential to identify functional connectivity among retinal neurons. For this purpose, imaging neuronal activity through fluorescent indicator proteins has been a promising approach, offering simultaneous measurements of neuronal activities from different regions of the circuit. In this study, we used genetically encoded indicatorsBongwoori-R3 for voltage or GCaMP6f for calciumto visualize membrane voltage or calcium dynamics, respectively, as spatial maps within individual retinal ganglion cells from retinal tissues of photoreceptor-degenerated <i>rd1</i> mice. Retinal voltage imaging was able to show current-evoked somatic spiking as well as subthreshold voltage changes, while calcium imaging showed changes in calcium concentrations evoked by current pulses in retinal ganglion cells. These results indicate that the combination of fluorescent protein sensors and high-speed imaging methods permits the imaging of electrical activity with cellular precision and millisecond resolution. Hence, we expect our method will provide a potent experimental platform for the study of retinal signaling pathways, as well as the development of retinal stimulation strategies in visual prosthesis.2025-11-24T17:38:16ZDatasetMediainfo:eu-repo/semantics/publishedVersiondataset10.1021/acschemneuro.5c00740.s003https://figshare.com/articles/media/Imaging_Electrical_Activity_of_Retinal_Ganglion_Cells_with_Fluorescent_Voltage_and_Calcium_Indicator_Proteins_in_Retinal_Degenerative_i_rd1_i_Blind_Mice/30696122CC BY-NC 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306961222025-11-24T17:38:16Z |
| spellingShingle | Imaging Electrical Activity of Retinal Ganglion Cells with Fluorescent Voltage and Calcium Indicator Proteins in Retinal Degenerative <i>rd1</i> Blind Mice Younginha Jung (22676081) Biophysics Biochemistry Medicine Microbiology Cell Biology Neuroscience Physiology Biotechnology Developmental Biology Chemical Sciences not elsewhere classified potent experimental platform offering simultaneous measurements evoked somatic spiking fluorescent protein sensors fluorescent indicator proteins retinal stimulation strategies retinal signaling pathways retinal ganglion cells calcium indicator proteins calcium concentrations evoked visualize membrane voltage subthreshold voltage changes imaging neuronal activity imaging electrical activity retinal voltage imaging electrical activity fluorescent voltage retinal tissues retinal network retinal degenerative neuronal activities calcium calcium dynamics visual prosthesis show current results indicate rd1 </ promising approach millisecond resolution different regions current pulses cellular precision |
| status_str | publishedVersion |
| title | Imaging Electrical Activity of Retinal Ganglion Cells with Fluorescent Voltage and Calcium Indicator Proteins in Retinal Degenerative <i>rd1</i> Blind Mice |
| title_full | Imaging Electrical Activity of Retinal Ganglion Cells with Fluorescent Voltage and Calcium Indicator Proteins in Retinal Degenerative <i>rd1</i> Blind Mice |
| title_fullStr | Imaging Electrical Activity of Retinal Ganglion Cells with Fluorescent Voltage and Calcium Indicator Proteins in Retinal Degenerative <i>rd1</i> Blind Mice |
| title_full_unstemmed | Imaging Electrical Activity of Retinal Ganglion Cells with Fluorescent Voltage and Calcium Indicator Proteins in Retinal Degenerative <i>rd1</i> Blind Mice |
| title_short | Imaging Electrical Activity of Retinal Ganglion Cells with Fluorescent Voltage and Calcium Indicator Proteins in Retinal Degenerative <i>rd1</i> Blind Mice |
| title_sort | Imaging Electrical Activity of Retinal Ganglion Cells with Fluorescent Voltage and Calcium Indicator Proteins in Retinal Degenerative <i>rd1</i> Blind Mice |
| topic | Biophysics Biochemistry Medicine Microbiology Cell Biology Neuroscience Physiology Biotechnology Developmental Biology Chemical Sciences not elsewhere classified potent experimental platform offering simultaneous measurements evoked somatic spiking fluorescent protein sensors fluorescent indicator proteins retinal stimulation strategies retinal signaling pathways retinal ganglion cells calcium indicator proteins calcium concentrations evoked visualize membrane voltage subthreshold voltage changes imaging neuronal activity imaging electrical activity retinal voltage imaging electrical activity fluorescent voltage retinal tissues retinal network retinal degenerative neuronal activities calcium calcium dynamics visual prosthesis show current results indicate rd1 </ promising approach millisecond resolution different regions current pulses cellular precision |