Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain.

(A) Left panel, images of plaque assays of the RNG2-mAID strain/ Δrng2 strain and TIR1 in the presence or absence of auxin (IAA). The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journa...

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Xehetasun bibliografikoak
Egile nagusia:	Romuald Haase (22339777) (author)
Beste egile batzuk:	Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
Argitaratua:	2025
Gaiak:	Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves
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Gaia:	<p><b>(A)</b> Left panel, images of plaque assays of the RNG2-mAID strain/ Δ<i>rng2</i> strain and TIR1 in the presence or absence of auxin (IAA). The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(B)</b> Two additional replicates of ethanol-triggered microneme secretion assessed by western blot. ESA = Excreted secreted antigens. <b>(C)</b> Quantification of the egress of the RNG2-mAID strain in the presence or absence of auxin (IAA). One hundred parasites were counted per replicate, and 3 biological replicates were performed. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(D)</b> Gliding trail assay of RNG2-mAID; gliding motility was determined via visualization of the SAG1 trail after BIPPO induction. <b>(E)</b> Quantification of the invasion of the RNG2-mAID strain in the presence or absence of auxin (IAA). One hundred parasites were counted per replicate, and 3 biological replicates were performed. Statistical analysis was performed via one-way ANOVA, and <i>p</i> < 0.05 was considered significant and is plotted in the figure. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>.</p> <p>(TIFF)</p>

Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain.

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