Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain.
<p><b>(A)</b> Left panel, images of plaque assays of the RNG2-mAID strain/ Δ<i>rng2</i> strain and TIR1 in the presence or absence of auxin (IAA). The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journa...
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| _version_ | 1849927642251264000 |
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| author | Romuald Haase (22339777) |
| author2 | Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| author2_role | author author author author author author author author author author |
| author_facet | Romuald Haase (22339777) Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| author_role | author |
| dc.creator.none.fl_str_mv | Romuald Haase (22339777) Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| dc.date.none.fl_str_mv | 2025-11-24T18:30:51Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003506.s004 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Detachment_of_the_conoid_in_absence_of_RNG2_impairs_parasite_motility_and_invasion_additional_phenotyping_of_the_RNG2-mAID_strain_/30697323 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |
| dc.title.none.fl_str_mv | Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(A)</b> Left panel, images of plaque assays of the RNG2-mAID strain/ Δ<i>rng2</i> strain and TIR1 in the presence or absence of auxin (IAA). The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(B)</b> Two additional replicates of ethanol-triggered microneme secretion assessed by western blot. ESA = Excreted secreted antigens. <b>(C)</b> Quantification of the egress of the RNG2-mAID strain in the presence or absence of auxin (IAA). One hundred parasites were counted per replicate, and 3 biological replicates were performed. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(D)</b> Gliding trail assay of RNG2-mAID; gliding motility was determined via visualization of the SAG1 trail after BIPPO induction. <b>(E)</b> Quantification of the invasion of the RNG2-mAID strain in the presence or absence of auxin (IAA). One hundred parasites were counted per replicate, and 3 biological replicates were performed. Statistical analysis was performed via one-way ANOVA, and <i>p</i> < 0.05 was considered significant and is plotted in the figure. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>.</p> <p>(TIFF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_1f9aa20aaad842f86a5b293f82603848 |
| identifier_str_mv | 10.1371/journal.pbio.3003506.s004 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697323 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain.Romuald Haase (22339777)Bingjian Ren (22676577)Albert Tell i Puig (22676580)Alessandro Bonavoglia (19415718)Jean-Baptiste Marq (375239)Rémy Visentin (22676583)Nicolas Dos Santos Pacheco (22676586)Bohumil Maco (383443)Ricardo Mondragón-Flores (10737057)Oscar Vadas (2416228)Dominique Soldati-Favre (79026)BiophysicsBiochemistryMicrobiologyCell BiologyPhysiologyInfectious DiseasesEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedPhysical Sciences not elsewhere classifiedtoxoplasma gondii </remains apically anchoredheterologous expression systemsdiv >< papical polar ringbiochemical analysis revealedproper conoid anchorageensures conoid functionalityless parasites revealedhost cell invasionenable parasite motilitycontrolling parasite motilityalthough rng2 depletionintact conoid organelleorganelle traversesintact proteinconditional depletionbiochemical propertiesdetached conoidgliding motilityunstable proteinundergoes extrusiontwo structuresstrong candidatestriking detachmentstill unclearrng2 tethersrng2 ledrng2 emergesrhoptries followresilient bridgeprotein localizedplasma membranepivotal proteinlikely facilitatelarge coiledimmunoelectron microscopyfunctional activityenabling rng2electron tomographydependent extrusioncritical roleconcatenated assembliescomprehensive mutagenesiscoil domainscalcium waves<p><b>(A)</b> Left panel, images of plaque assays of the RNG2-mAID strain/ Δ<i>rng2</i> strain and TIR1 in the presence or absence of auxin (IAA). The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(B)</b> Two additional replicates of ethanol-triggered microneme secretion assessed by western blot. ESA = Excreted secreted antigens. <b>(C)</b> Quantification of the egress of the RNG2-mAID strain in the presence or absence of auxin (IAA). One hundred parasites were counted per replicate, and 3 biological replicates were performed. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(D)</b> Gliding trail assay of RNG2-mAID; gliding motility was determined via visualization of the SAG1 trail after BIPPO induction. <b>(E)</b> Quantification of the invasion of the RNG2-mAID strain in the presence or absence of auxin (IAA). One hundred parasites were counted per replicate, and 3 biological replicates were performed. Statistical analysis was performed via one-way ANOVA, and <i>p</i> < 0.05 was considered significant and is plotted in the figure. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>.</p> <p>(TIFF)</p>2025-11-24T18:30:51ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003506.s004https://figshare.com/articles/figure/Detachment_of_the_conoid_in_absence_of_RNG2_impairs_parasite_motility_and_invasion_additional_phenotyping_of_the_RNG2-mAID_strain_/30697323CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306973232025-11-24T18:30:51Z |
| spellingShingle | Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain. Romuald Haase (22339777) Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |
| status_str | publishedVersion |
| title | Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain. |
| title_full | Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain. |
| title_fullStr | Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain. |
| title_full_unstemmed | Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain. |
| title_short | Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain. |
| title_sort | Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain. |
| topic | Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |