The effect of oxamate on chromocenters and satellite transcripts.

The effect of oxamate on chromocenters and satellite transcripts.

<p><b>A</b>.Changes in centromeres composition during prophase 1. Pachytene, diplotene and diakinesis cells were stained with SCP3 (green) and CENPA, CENPC, CENPN and CREST antibodies (red). Note gradual appearance of CENPC and CENPN as compared with CENPA. <b>B</b>. Ch...

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Main Author: Nina Mayorek (241888) (author)
Other Authors: Miriam Schlossberg (21696644) (author), Yousef Mansour (21696647) (author), Nir Pillar (5249612) (author), Ilan Stein (131429) (author), Fatima Mushasha (21696650) (author), Guy Baziza Paz (21696653) (author), Eleonora Medvedev (21696656) (author), Zakhariya Manevitch (21696659) (author), Julia Menzel (12879920) (author), Elina Aizenshtein (21696662) (author), Boris Sarvin (8232306) (author), Nikita Sarvin (21696665) (author), Erwin Goldberg (2868524) (author), Bryan A. Niedenberger (21696668) (author), Christopher B. Geyer (13840432) (author), Tomer Shlomi (22999) (author), Michael Klutstein (22329) (author), Eli Pikarsky (103960) (author)
Published: 2025
Subjects:
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Biological Sciences not elsewhere classified
spindle assembly checkpoint
satellite rna expression
sac ), suggesting
producing functional spermatozoa
histone methylation changes
including chromocenter clustering
2hg ), yet
regulates centromere condensation
hydroxyglutarate regulates centromere
chromocenter condensation
centromere dysfunction
vivo results
synaptonemal complex
localizing along
heterochromatin conformation
epigenetic modifications
diplotene stages
acute depletion
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Summary:<p><b>A</b>.Changes in centromeres composition during prophase 1. Pachytene, diplotene and diakinesis cells were stained with SCP3 (green) and CENPA, CENPC, CENPN and CREST antibodies (red). Note gradual appearance of CENPC and CENPN as compared with CENPA. <b>B</b>. Chromocenters demarcated by HP1α antibody are more prominent in diplotene cells as compared to pachytene cells. <b>C-H</b> PDD cells were isolated from two groups: mice injected once with oxamate (1.3g/kg) and sacrificed after 24 hours (Ox) and mice injected with vehicle (Ctr). <b>C-F</b>. Chromocenters of diplotene cells (n = 60, analyzed with a t-test) stained with H3K9me3 were evaluated for the following parameters: area (<b>C</b>), staining intensity (<b>D</b>), DAPI intensity (<b>E</b>), and the ratio of H3K9me3 to DAPI intensities (<b>F</b>). <b>G-H</b> qPCR performed for minor (<b>G</b>) or major (<b>H</b>) satellite RNA. Data represent mean±SE from 3 experiments, t-test.</p> <p>(TIF)</p>

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