L-2HG and LDHC guarded centromere morphology in diakinesis cells.
<p>(A) Experimental Design (Created with Biorender). This experiment aimed to study the effect of 2HG on compacting oxamate-decompacted diakinesis centromeres. Diakinesis cells were isolated from two groups: mice injected once with oxamate (1.3 g/kg) and sacrificed after 24 hours (Ox) and mice...
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2025
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| _version_ | 1851484667174191104 |
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| author | Nina Mayorek (241888) |
| author2 | Miriam Schlossberg (21696644) Yousef Mansour (21696647) Nir Pillar (5249612) Ilan Stein (131429) Fatima Mushasha (21696650) Guy Baziza Paz (21696653) Eleonora Medvedev (21696656) Zakhariya Manevitch (21696659) Julia Menzel (12879920) Elina Aizenshtein (21696662) Boris Sarvin (8232306) Nikita Sarvin (21696665) Erwin Goldberg (2868524) Bryan A. Niedenberger (21696668) Christopher B. Geyer (13840432) Tomer Shlomi (22999) Michael Klutstein (22329) Eli Pikarsky (103960) |
| author2_role | author author author author author author author author author author author author author author author author author author |
| author_facet | Nina Mayorek (241888) Miriam Schlossberg (21696644) Yousef Mansour (21696647) Nir Pillar (5249612) Ilan Stein (131429) Fatima Mushasha (21696650) Guy Baziza Paz (21696653) Eleonora Medvedev (21696656) Zakhariya Manevitch (21696659) Julia Menzel (12879920) Elina Aizenshtein (21696662) Boris Sarvin (8232306) Nikita Sarvin (21696665) Erwin Goldberg (2868524) Bryan A. Niedenberger (21696668) Christopher B. Geyer (13840432) Tomer Shlomi (22999) Michael Klutstein (22329) Eli Pikarsky (103960) |
| author_role | author |
| dc.creator.none.fl_str_mv | Nina Mayorek (241888) Miriam Schlossberg (21696644) Yousef Mansour (21696647) Nir Pillar (5249612) Ilan Stein (131429) Fatima Mushasha (21696650) Guy Baziza Paz (21696653) Eleonora Medvedev (21696656) Zakhariya Manevitch (21696659) Julia Menzel (12879920) Elina Aizenshtein (21696662) Boris Sarvin (8232306) Nikita Sarvin (21696665) Erwin Goldberg (2868524) Bryan A. Niedenberger (21696668) Christopher B. Geyer (13840432) Tomer Shlomi (22999) Michael Klutstein (22329) Eli Pikarsky (103960) |
| dc.date.none.fl_str_mv | 2025-07-10T17:56:42Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pgen.1011785.g005 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/L-2HG_and_LDHC_guarded_centromere_morphology_in_diakinesis_cells_/29537597 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cell Biology Genetics Molecular Biology Developmental Biology Biological Sciences not elsewhere classified spindle assembly checkpoint satellite rna expression sac ), suggesting producing functional spermatozoa histone methylation changes including chromocenter clustering 2hg ), yet regulates centromere condensation hydroxyglutarate regulates centromere chromocenter condensation centromere dysfunction vivo results synaptonemal complex localizing along heterochromatin conformation epigenetic modifications diplotene stages acute depletion |
| dc.title.none.fl_str_mv | L-2HG and LDHC guarded centromere morphology in diakinesis cells. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>(A) Experimental Design (Created with Biorender). This experiment aimed to study the effect of 2HG on compacting oxamate-decompacted diakinesis centromeres. Diakinesis cells were isolated from two groups: mice injected once with oxamate (1.3 g/kg) and sacrificed after 24 hours (Ox) and mice injected with vehicle-only (Ctr). Diakinesis cells from oxamate-injected mice, displaying enlarged centromeres (see B), were subjected to a 10-minute incubation at 32°C with 0.6 mM octyl L/D-2HG, while cells from control mice were treated under identical conditions with vehicle only. After incubation, nuclear spreads were prepared and immunostained for various markers: CREST/SCP3 (B-D, K), LDHC/SCP3 (H-J), CENP-C (E), CENP-N (F), and CENP-A (G). Representative images of E-G are presented in (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011785#pgen.1011785.s005" target="_blank">S5 Fig</a>). Cross-sectional area measurements of centromeres immunostained with the above antibodies were conducted (B-C, E-H). Another group of diakinesis cells underwent the same procedure but were incubated with 5 mM lactate instead of 2HG, and centromeres were immunostained with CREST antibodies (N). Each point in (B-C, E-H, and N) represents the mean area of all centromeres in one cell, while in (L-M), each point represents the mean staining intensity of centromeres in one cell. Data are presented as mean ± SE of n = 30-60 cells in each treatment. Statistical analyses were conducted using t-test for (B) and Tukey’s multiple comparisons test for (C-H and L-N). Immunostained areas were measured using Zeiss ZEN 3.3 software and expressed as a fraction of the mean of all experimental groups in arbitrary units (a.u). (D and I) Confocal images of centromere areas immunostained with CREST antibodies (quantified in C) and LDHC (quantified in H) are depicted. Scale bars in D and I = 10 µm. (J-K) High-resolution images of centromeres captured by the N-SIM microscope are provided, scale bar = 1 µm.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_20d06676b51c81d7533bd8f17c9c77d4 |
| identifier_str_mv | 10.1371/journal.pgen.1011785.g005 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/29537597 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | L-2HG and LDHC guarded centromere morphology in diakinesis cells.Nina Mayorek (241888)Miriam Schlossberg (21696644)Yousef Mansour (21696647)Nir Pillar (5249612)Ilan Stein (131429)Fatima Mushasha (21696650)Guy Baziza Paz (21696653)Eleonora Medvedev (21696656)Zakhariya Manevitch (21696659)Julia Menzel (12879920)Elina Aizenshtein (21696662)Boris Sarvin (8232306)Nikita Sarvin (21696665)Erwin Goldberg (2868524)Bryan A. Niedenberger (21696668)Christopher B. Geyer (13840432)Tomer Shlomi (22999)Michael Klutstein (22329)Eli Pikarsky (103960)Cell BiologyGeneticsMolecular BiologyDevelopmental BiologyBiological Sciences not elsewhere classifiedspindle assembly checkpointsatellite rna expressionsac ), suggestingproducing functional spermatozoahistone methylation changesincluding chromocenter clustering2hg ), yetregulates centromere condensationhydroxyglutarate regulates centromerechromocenter condensationcentromere dysfunctionvivo resultssynaptonemal complexlocalizing alongheterochromatin conformationepigenetic modificationsdiplotene stagesacute depletion<p>(A) Experimental Design (Created with Biorender). This experiment aimed to study the effect of 2HG on compacting oxamate-decompacted diakinesis centromeres. Diakinesis cells were isolated from two groups: mice injected once with oxamate (1.3 g/kg) and sacrificed after 24 hours (Ox) and mice injected with vehicle-only (Ctr). Diakinesis cells from oxamate-injected mice, displaying enlarged centromeres (see B), were subjected to a 10-minute incubation at 32°C with 0.6 mM octyl L/D-2HG, while cells from control mice were treated under identical conditions with vehicle only. After incubation, nuclear spreads were prepared and immunostained for various markers: CREST/SCP3 (B-D, K), LDHC/SCP3 (H-J), CENP-C (E), CENP-N (F), and CENP-A (G). Representative images of E-G are presented in (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011785#pgen.1011785.s005" target="_blank">S5 Fig</a>). Cross-sectional area measurements of centromeres immunostained with the above antibodies were conducted (B-C, E-H). Another group of diakinesis cells underwent the same procedure but were incubated with 5 mM lactate instead of 2HG, and centromeres were immunostained with CREST antibodies (N). Each point in (B-C, E-H, and N) represents the mean area of all centromeres in one cell, while in (L-M), each point represents the mean staining intensity of centromeres in one cell. Data are presented as mean ± SE of n = 30-60 cells in each treatment. Statistical analyses were conducted using t-test for (B) and Tukey’s multiple comparisons test for (C-H and L-N). Immunostained areas were measured using Zeiss ZEN 3.3 software and expressed as a fraction of the mean of all experimental groups in arbitrary units (a.u). (D and I) Confocal images of centromere areas immunostained with CREST antibodies (quantified in C) and LDHC (quantified in H) are depicted. Scale bars in D and I = 10 µm. (J-K) High-resolution images of centromeres captured by the N-SIM microscope are provided, scale bar = 1 µm.</p>2025-07-10T17:56:42ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pgen.1011785.g005https://figshare.com/articles/figure/L-2HG_and_LDHC_guarded_centromere_morphology_in_diakinesis_cells_/29537597CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/295375972025-07-10T17:56:42Z |
| spellingShingle | L-2HG and LDHC guarded centromere morphology in diakinesis cells. Nina Mayorek (241888) Cell Biology Genetics Molecular Biology Developmental Biology Biological Sciences not elsewhere classified spindle assembly checkpoint satellite rna expression sac ), suggesting producing functional spermatozoa histone methylation changes including chromocenter clustering 2hg ), yet regulates centromere condensation hydroxyglutarate regulates centromere chromocenter condensation centromere dysfunction vivo results synaptonemal complex localizing along heterochromatin conformation epigenetic modifications diplotene stages acute depletion |
| status_str | publishedVersion |
| title | L-2HG and LDHC guarded centromere morphology in diakinesis cells. |
| title_full | L-2HG and LDHC guarded centromere morphology in diakinesis cells. |
| title_fullStr | L-2HG and LDHC guarded centromere morphology in diakinesis cells. |
| title_full_unstemmed | L-2HG and LDHC guarded centromere morphology in diakinesis cells. |
| title_short | L-2HG and LDHC guarded centromere morphology in diakinesis cells. |
| title_sort | L-2HG and LDHC guarded centromere morphology in diakinesis cells. |
| topic | Cell Biology Genetics Molecular Biology Developmental Biology Biological Sciences not elsewhere classified spindle assembly checkpoint satellite rna expression sac ), suggesting producing functional spermatozoa histone methylation changes including chromocenter clustering 2hg ), yet regulates centromere condensation hydroxyglutarate regulates centromere chromocenter condensation centromere dysfunction vivo results synaptonemal complex localizing along heterochromatin conformation epigenetic modifications diplotene stages acute depletion |