Extinction coefficient measurement of NagS.

Extinction coefficient measurement of NagS.

<p>(<b>a</b>) This standard curve of GlcNAc-6P was obtained by plotting the concentration of GlcNAc-6P (1, 1.67, 2.67, 3.33, 4, and 5 mM) with the corresponding peak area detected in the LC–MS spectrum. (<b>b</b>) Area of the GlcNAc-6P peak after the reaction catalyzed...

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Main Author: Chao Li (145513) (author)
Other Authors: Mia Urem (22683580) (author), Ioli Kotsogianni (9930187) (author), Josephine Lau (20442343) (author), Chao Du (288036) (author), Somayah S. Elsayed (9192571) (author), Nathaniel I. Martin (847378) (author), Iain W. McNae (840777) (author), Patrick Voskamp (2311429) (author), Christoph Mayer (57204) (author), Sébastien Rigali (724082) (author), Navraj Pannu (10163763) (author), Jan Pieter Abrahams (1429531) (author), Lennart Schada von Borzyskowski (22683583) (author), Gilles P. van Wezel (7838948) (author)
Published: 2025
Subjects:
Biochemistry
Microbiology
Ecology
Developmental Biology
Inorganic Chemistry
Biological Sciences not elsewhere classified
controls nutrient signaling
also revealed 6
highlighted key residues
glcnac sensing requires
toxicity pathway dependent
6p deacetylase naga
novel glcnac 6
key role
substrate glcnac
novel enzyme
work uncovers
wall leads
unprecedented reaction
substrate mycelium
structural analogue
streptomycetaceae </
streptomyces </
revolves around
promiscuous activity
nature ’
n </
multicellular lifestyle
metabolic checkpoint
medicine makers
lytic dismantling
landmark event
hyphal cell
highly conserved
growth media
function analysis
clinical antibiotics
central metabolism
catalytic inhibitor
antibiotic production
active site
>- acetylglucosamine
6p dehydratase
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Summary:<p>(<b>a</b>) This standard curve of GlcNAc-6P was obtained by plotting the concentration of GlcNAc-6P (1, 1.67, 2.67, 3.33, 4, and 5 mM) with the corresponding peak area detected in the LC–MS spectrum. (<b>b</b>) Area of the GlcNAc-6P peak after the reaction catalyzed by NagS. The concentration of remaining GlcNAc-6P is calculated to be 2.15 mM. The data underlying this Figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003514#pbio.3003514.s022" target="_blank">S1 Data</a>.</p> <p>(TIF)</p>

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