Primers Used in This Study.
<div><p>The growing prevalence of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) infections, coupled with the increasing resistance to existing antibiotics, underscores the critical need for novel therapeutic approaches to combat this pathogen. In this study, the r...
Saved in:
| 主要作者: | |
|---|---|
| 其他作者: | , , , |
| 出版: |
2025
|
| 主题: | |
| 标签: |
添加标签
没有标签, 成为第一个标记此记录!
|
| _version_ | 1849927628578881536 |
|---|---|
| author | Jianhua Liao (1393228) |
| author2 | Jun Cheng (194158) Baoqing Liu (1508821) Yuzhi Shao (22683349) Chunyan Meng (5993228) |
| author2_role | author author author author |
| author_facet | Jianhua Liao (1393228) Jun Cheng (194158) Baoqing Liu (1508821) Yuzhi Shao (22683349) Chunyan Meng (5993228) |
| author_role | author |
| dc.creator.none.fl_str_mv | Jianhua Liao (1393228) Jun Cheng (194158) Baoqing Liu (1508821) Yuzhi Shao (22683349) Chunyan Meng (5993228) |
| dc.date.none.fl_str_mv | 2025-11-25T18:28:11Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0337292.t002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Primers_Used_in_This_Study_/30713770 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence |
| dc.title.none.fl_str_mv | Primers Used in This Study. |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <div><p>The growing prevalence of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) infections, coupled with the increasing resistance to existing antibiotics, underscores the critical need for novel therapeutic approaches to combat this pathogen. In this study, the role of <i>yqhG</i>, a conserved gene encoding a periplasmic protein, in MRSA virulence and stress adaptation was investigated. <i>yqhG</i> deletion in MRSA significantly attenuated virulence in a murine infection model, leading to reduced bacterial burden in infected organs and improved host survival. In vitro, the <i>yqhG</i> mutant exhibited impaired membrane integrity, reduced motility, and increased sensitivity to oxidative stress, but did not affect biofilm formation. These defects were fully restored upon genetic complementation. These findings highlight the critical role of <i>yqhG</i> in maintaining MRSA’s ability to withstand host-imposed stresses, suggesting that <i>yqhG</i> is a key determinant of MRSA pathogenesis. The study provides new insights into the stress-defense mechanisms employed by MRSA and underscores <i>yqhG</i> as a potential target for therapeutic strategies aimed at combating MRSA infections.</p></div> |
| eu_rights_str_mv | openAccess |
| id | Manara_24e380d73c7e5b358754c53b4e5cd8e5 |
| identifier_str_mv | 10.1371/journal.pone.0337292.t002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30713770 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Primers Used in This Study.Jianhua Liao (1393228)Jun Cheng (194158)Baoqing Liu (1508821)Yuzhi Shao (22683349)Chunyan Meng (5993228)MedicineMicrobiologyCell BiologyBiotechnologyEvolutionary BiologyEcologyScience PolicyInfectious DiseasesVirologyEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedtherapeutic strategies aimednovel therapeutic approachesmurine infection modeldiv >< pdefense mechanisms employedconserved gene encodingcompromising membrane stabilityaffect biofilm formationstaphylococcus aureus </reduced bacterial burdenimproved host survivalmaintaining mrsa ’oxidative stress resistanceyqhg </withstand hostreduced motilityoxidative stressincreasing resistancestress adaptationpotential targetperiplasmic proteinmrsa virulencemrsa pathogenesiskey determinantinfected organsincreased sensitivityimposed stressesgrowing prevalencefindings highlightexisting antibioticscritical needattenuates virulence<div><p>The growing prevalence of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) infections, coupled with the increasing resistance to existing antibiotics, underscores the critical need for novel therapeutic approaches to combat this pathogen. In this study, the role of <i>yqhG</i>, a conserved gene encoding a periplasmic protein, in MRSA virulence and stress adaptation was investigated. <i>yqhG</i> deletion in MRSA significantly attenuated virulence in a murine infection model, leading to reduced bacterial burden in infected organs and improved host survival. In vitro, the <i>yqhG</i> mutant exhibited impaired membrane integrity, reduced motility, and increased sensitivity to oxidative stress, but did not affect biofilm formation. These defects were fully restored upon genetic complementation. These findings highlight the critical role of <i>yqhG</i> in maintaining MRSA’s ability to withstand host-imposed stresses, suggesting that <i>yqhG</i> is a key determinant of MRSA pathogenesis. The study provides new insights into the stress-defense mechanisms employed by MRSA and underscores <i>yqhG</i> as a potential target for therapeutic strategies aimed at combating MRSA infections.</p></div>2025-11-25T18:28:11ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.1371/journal.pone.0337292.t002https://figshare.com/articles/dataset/Primers_Used_in_This_Study_/30713770CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307137702025-11-25T18:28:11Z |
| spellingShingle | Primers Used in This Study. Jianhua Liao (1393228) Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence |
| status_str | publishedVersion |
| title | Primers Used in This Study. |
| title_full | Primers Used in This Study. |
| title_fullStr | Primers Used in This Study. |
| title_full_unstemmed | Primers Used in This Study. |
| title_short | Primers Used in This Study. |
| title_sort | Primers Used in This Study. |
| topic | Medicine Microbiology Cell Biology Biotechnology Evolutionary Biology Ecology Science Policy Infectious Diseases Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified therapeutic strategies aimed novel therapeutic approaches murine infection model div >< p defense mechanisms employed conserved gene encoding compromising membrane stability affect biofilm formation staphylococcus aureus </ reduced bacterial burden improved host survival maintaining mrsa ’ oxidative stress resistance yqhg </ withstand host reduced motility oxidative stress increasing resistance stress adaptation potential target periplasmic protein mrsa virulence mrsa pathogenesis key determinant infected organs increased sensitivity imposed stresses growing prevalence findings highlight existing antibiotics critical need attenuates virulence |