Full, uncropped Western blot images corresponding to Fig 4A.

Full, uncropped Western blot images corresponding to Fig 4A.

<p>Full-size, unprocessed images of the Western blots used for the analysis presented in Figure 4A. (A) The membrane was probed with an anti-FLAG antibody to detect LdrA-FLAG. (B) A parallel membrane, prepared with an identical set of samples, was probed with an anti-OmpA antibody. Lane order...

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Príomhchruthaitheoir:	Yuto Nakamura (1493086) (author)
Rannpháirtithe:	Yoshihiro Yamaguchi (329528) (author)
Foilsithe / Cruthaithe:	2025
Ábhair:	Biochemistry Microbiology Cell Biology Genetics Molecular Biology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified transient growth arrest escherichia coli </ div >< p cells subsequently recover ldr toxin family small membrane protein forming toxin ldra forming toxin damaging toxin accessory protein ldra toxicity ldra results ldra requires including ldra >, ldra suppressive activity results indicate present study novel function molecular basis membrane localization known role key factor homolog ldrd genetic screen findings reveal cytoplasmic domains
Clibeanna:	Cuir clib leis Níl clibeanna ann, Bí ar an gcéad duine le clib a chur leis an taifead seo!

Míreanna comhchosúla

YajC recognition of LdrA requires both transmembrane and cytoplasmic domains.
de réir: Yuto Nakamura (1493086)
Foilsithe / Cruthaithe: (2025)

YajC Specifically Neutralizes Ldr Family Toxins.
de réir: Yuto Nakamura (1493086)
Foilsithe / Cruthaithe: (2025)

YajC co-expression does not alter the membrane localization or abundance of LdrA-FLAG.
de réir: Yuto Nakamura (1493086)
Foilsithe / Cruthaithe: (2025)

Overexpression of YajC is sufficient to alleviate LdrA-induced toxicity.
de réir: Yuto Nakamura (1493086)
Foilsithe / Cruthaithe: (2025)

Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor.
de réir: Yuto Nakamura (1493086)
Foilsithe / Cruthaithe: (2025)

LdrA expression causes transient growth arrest and the FLAG tag does not affect its toxicity.
de réir: Yuto Nakamura (1493086)
Foilsithe / Cruthaithe: (2025)

YajC has no effect on unrelated TA toxins.
de réir: Yuto Nakamura (1493086)
Foilsithe / Cruthaithe: (2025)

Inducible overexpression of YajC-HA.
de réir: Yuto Nakamura (1493086)
Foilsithe / Cruthaithe: (2025)

Controls for LdrA toxicity and recovery experiments.
de réir: Yuto Nakamura (1493086)
Foilsithe / Cruthaithe: (2025)

All raw data
de réir: li lei (22680179)
Foilsithe / Cruthaithe: (2025)

The U sequence method.
de réir: Pierre Angelvy (22683450)
Foilsithe / Cruthaithe: (2025)

Provisional study schedule of MUSIBOT.
de réir: Pierre Angelvy (22683450)
Foilsithe / Cruthaithe: (2025)

Participant timeline: Schedule of enrollment, interventions, and assessments.
de réir: Pierre Angelvy (22683450)
Foilsithe / Cruthaithe: (2025)

Western blot of purified p33 protein transiently expressed in <i>N. benthamiana</i> displaying protein bands which correspond to the expected sizes of p33 monomers, dimers, and tetramers.
de réir: Vicken Aknadibossian (22676694)
Foilsithe / Cruthaithe: (2025)

The file compiles all original, uncropped, and unprocessed data collected in the experiments, including colony diameter, conidia production, conidia size, septa of conidia, DON production, the disease indexes, relative gene expression level.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Antibody information used for Western blot.
de réir: Yingying Chen (500738)
Foilsithe / Cruthaithe: (2025)

Original underlying data in support of Fig 3. This file includes the quantitative data and microscopy images for panels A-C, the original uncropped Western blot gels for Figs A-B, and the original uncropped western blot for the Claudin-5 panel in Fig 3C.
de réir: Yanjiang Li (584137)
Foilsithe / Cruthaithe: (2025)

Summary of the general phenotype of SH3 domain-containing gene mutants.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

SH3 domain-containing genes and the subcellular localization of their respective products in <i>F. graminearum.</i>
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

The IDRs in SH3 domain-containing proteins are important for the fusion of cytoplasmic protein condensates.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

SH3 domain-containing proteins exhibit features of phase separation in <i>F. graminearum.</i>
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Effects of <i>FgHOF1</i> deletion on cytoskeletal and nuclear distribution.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Co-localization of FgAip5-GFP at hyphal tips and various SH3-domain proteins at membrane-associated structures.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Subcellular localizations of SH3 domain-containing proteins in <i>F. graminearum</i> hyphae.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Role of SH3 domain-containing proteins on deoxynivalenol (DON) production in <i>F. graminearum.</i>
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Effect of SH3 domain gene deletions on the pathogenicity of <i>F. graminearum.</i>
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Influence of SH3 domain-containing proteins on sexual development of <i>F. graminearum.</i>
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Conidiation and conidial morphology of the SH3 domain-containing gene mutants.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Impact of SH3 domain-containing proteins on the vegetative growth of <i>F. graminearum.</i>
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Time-lapse images showing the fusion of two FgRvs167<sup>ΔIDR</sup>-GFP protein condensates in young mycelia.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Time-lapse images showing the fusion of two FgPin3<sup>ΔIDR2</sup>-GFP protein condensates in young mycelia.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Time-lapse images showing the fusion of two FgPin<sup>ΔIDR1</sup>-GFP protein condensates in young mycelia.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Time-lapse images showing the fusion of two FgBoi1-GFP protein condensates in young mycelia.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Time-lapse images showing the fusion of two FgRvs167-GFP protein condensates in young mycelia.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Time-lapse images showing the fusion of two FgPin3-GFP protein condensates in young mycelia.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgRvs167<sup>ΔIDR</sup>-GFP.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgBoi1-GFP.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgRvs167-GFP.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgPin3<sup>ΔIDR2</sup>-GFP.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

Live-cell images of fluorescence recovery after photobleaching (FRAP) experiments in the strain expressing FgPin3<sup>ΔIDR1</sup>-GFP.
de réir: Yakubu Saddeeq Abubakar (5558399)
Foilsithe / Cruthaithe: (2025)

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