RNG2 spans between the APR and the conoid, forming 22 tethers.
<p><b>(A)</b> Illustration of endogenous tagging of RNG2, including three epitope tags and a minimal auxin inducible (mAID) motif. The estimation of the protein length corresponding to the distance coverage of the central part of the protein in linear conformation. <b>(B)<...
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| _version_ | 1849927642210369536 |
|---|---|
| author | Romuald Haase (22339777) |
| author2 | Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| author2_role | author author author author author author author author author author |
| author_facet | Romuald Haase (22339777) Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| author_role | author |
| dc.creator.none.fl_str_mv | Romuald Haase (22339777) Bingjian Ren (22676577) Albert Tell i Puig (22676580) Alessandro Bonavoglia (19415718) Jean-Baptiste Marq (375239) Rémy Visentin (22676583) Nicolas Dos Santos Pacheco (22676586) Bohumil Maco (383443) Ricardo Mondragón-Flores (10737057) Oscar Vadas (2416228) Dominique Soldati-Favre (79026) |
| dc.date.none.fl_str_mv | 2025-11-24T18:31:07Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3003506.g003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/RNG2_spans_between_the_APR_and_the_conoid_forming_22_tethers_/30697359 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |
| dc.title.none.fl_str_mv | RNG2 spans between the APR and the conoid, forming 22 tethers. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(A)</b> Illustration of endogenous tagging of RNG2, including three epitope tags and a minimal auxin inducible (mAID) motif. The estimation of the protein length corresponding to the distance coverage of the central part of the protein in linear conformation. <b>(B)</b> Protein depletion after 48 h of auxin (IAA) treatment assessed by western blot. <b>(C)</b> Western blot of parasite lysate collected in intracellular or extracellular state <b>(D)</b> U-ExM image of the locations of RNG2-mAID depending on the tag insertion and RNG2 polyclonal antibody (recognizing the central region). Images were taken with extruded and retracted conoids. Scale bar = 3 μm. <b>(E)</b> Iterative expansion microscopy (iU-ExM) of the N-terminal (Myc), middle (Ty), and C-terminal (HA) tags of RNG2-mAID. Scale bar = 3 μm. Right panel: Distribution of the numbers of dots counted for the anti-HA staining across 13 images. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(F) Left panel:</b> Electron microscopy images of the <i>T</i>oxoplasma <i>gondii</i> apical complex as an ultrastructure control. <b>Right panel:</b> Immunoelectron microscopy images obtained with the α-GAC and α-RNG2 antibody. Red arrows = gold particles. Scale bar = 100 nm.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_291c783183bdaa79699d4e918574ac48 |
| identifier_str_mv | 10.1371/journal.pbio.3003506.g003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697359 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | RNG2 spans between the APR and the conoid, forming 22 tethers.Romuald Haase (22339777)Bingjian Ren (22676577)Albert Tell i Puig (22676580)Alessandro Bonavoglia (19415718)Jean-Baptiste Marq (375239)Rémy Visentin (22676583)Nicolas Dos Santos Pacheco (22676586)Bohumil Maco (383443)Ricardo Mondragón-Flores (10737057)Oscar Vadas (2416228)Dominique Soldati-Favre (79026)BiophysicsBiochemistryMicrobiologyCell BiologyPhysiologyInfectious DiseasesEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedPhysical Sciences not elsewhere classifiedtoxoplasma gondii </remains apically anchoredheterologous expression systemsdiv >< papical polar ringbiochemical analysis revealedproper conoid anchorageensures conoid functionalityless parasites revealedhost cell invasionenable parasite motilitycontrolling parasite motilityalthough rng2 depletionintact conoid organelleorganelle traversesintact proteinconditional depletionbiochemical propertiesdetached conoidgliding motilityunstable proteinundergoes extrusiontwo structuresstrong candidatestriking detachmentstill unclearrng2 tethersrng2 ledrng2 emergesrhoptries followresilient bridgeprotein localizedplasma membranepivotal proteinlikely facilitatelarge coiledimmunoelectron microscopyfunctional activityenabling rng2electron tomographydependent extrusioncritical roleconcatenated assembliescomprehensive mutagenesiscoil domainscalcium waves<p><b>(A)</b> Illustration of endogenous tagging of RNG2, including three epitope tags and a minimal auxin inducible (mAID) motif. The estimation of the protein length corresponding to the distance coverage of the central part of the protein in linear conformation. <b>(B)</b> Protein depletion after 48 h of auxin (IAA) treatment assessed by western blot. <b>(C)</b> Western blot of parasite lysate collected in intracellular or extracellular state <b>(D)</b> U-ExM image of the locations of RNG2-mAID depending on the tag insertion and RNG2 polyclonal antibody (recognizing the central region). Images were taken with extruded and retracted conoids. Scale bar = 3 μm. <b>(E)</b> Iterative expansion microscopy (iU-ExM) of the N-terminal (Myc), middle (Ty), and C-terminal (HA) tags of RNG2-mAID. Scale bar = 3 μm. Right panel: Distribution of the numbers of dots counted for the anti-HA staining across 13 images. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.s008" target="_blank">S2 Data</a>. <b>(F) Left panel:</b> Electron microscopy images of the <i>T</i>oxoplasma <i>gondii</i> apical complex as an ultrastructure control. <b>Right panel:</b> Immunoelectron microscopy images obtained with the α-GAC and α-RNG2 antibody. Red arrows = gold particles. Scale bar = 100 nm.</p>2025-11-24T18:31:07ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pbio.3003506.g003https://figshare.com/articles/figure/RNG2_spans_between_the_APR_and_the_conoid_forming_22_tethers_/30697359CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306973592025-11-24T18:31:07Z |
| spellingShingle | RNG2 spans between the APR and the conoid, forming 22 tethers. Romuald Haase (22339777) Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |
| status_str | publishedVersion |
| title | RNG2 spans between the APR and the conoid, forming 22 tethers. |
| title_full | RNG2 spans between the APR and the conoid, forming 22 tethers. |
| title_fullStr | RNG2 spans between the APR and the conoid, forming 22 tethers. |
| title_full_unstemmed | RNG2 spans between the APR and the conoid, forming 22 tethers. |
| title_short | RNG2 spans between the APR and the conoid, forming 22 tethers. |
| title_sort | RNG2 spans between the APR and the conoid, forming 22 tethers. |
| topic | Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves |