RNG2 spans between the APR and the conoid, forming 22 tethers.

RNG2 spans between the APR and the conoid, forming 22 tethers.

<p><b>(A)</b> Illustration of endogenous tagging of RNG2, including three epitope tags and a minimal auxin inducible (mAID) motif. The estimation of the protein length corresponding to the distance coverage of the central part of the protein in linear conformation. <b>(B)<...

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Bibliographische Detailangaben
1. Verfasser:	Romuald Haase (22339777) (author)
Weitere Verfasser:	Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
Veröffentlicht:	2025
Schlagworte:	Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves
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Model RNG2 as a molecular tether for conoid apical anchorage.
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Phenotypic consequences of the conoid detachment.
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RNG2 depletion leads to conoid detachment from the apical pole of the parasite.
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Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain.
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RNG2 is functional only as intact, full-length protein.
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Investigation of RNG2 subdomain functions.
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Biochemical investigation of wild-type and processed RNG2.
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Reconstruction of the Fig 4E panel III.
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Reconstruction of the Fig 4E panel II.
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Reconstruction of the Fig 4E panel I.
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Source Numerical Data of this study.
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Additional information on antibodies and primers used in this study.
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