RNG2 spans between the APR and the conoid, forming 22 tethers.

RNG2 spans between the APR and the conoid, forming 22 tethers.

<p><b>(A)</b> Illustration of endogenous tagging of RNG2, including three epitope tags and a minimal auxin inducible (mAID) motif. The estimation of the protein length corresponding to the distance coverage of the central part of the protein in linear conformation. <b>(B)<...

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Bibliografische gegevens
Hoofdauteur:	Romuald Haase (22339777) (author)
Andere auteurs:	Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
Gepubliceerd in:	2025
Onderwerpen:	Biophysics Biochemistry Microbiology Cell Biology Physiology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Physical Sciences not elsewhere classified toxoplasma gondii </ remains apically anchored heterologous expression systems div >< p apical polar ring biochemical analysis revealed proper conoid anchorage ensures conoid functionality less parasites revealed host cell invasion enable parasite motility controlling parasite motility although rng2 depletion intact conoid organelle organelle traverses intact protein conditional depletion biochemical properties detached conoid gliding motility unstable protein undergoes extrusion two structures strong candidate striking detachment still unclear rng2 tethers rng2 led rng2 emerges rhoptries follow resilient bridge protein localized plasma membrane pivotal protein likely facilitate large coiled immunoelectron microscopy functional activity enabling rng2 electron tomography dependent extrusion critical role concatenated assemblies comprehensive mutagenesis coil domains calcium waves
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Model RNG2 as a molecular tether for conoid apical anchorage.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Phenotypic consequences of the conoid detachment.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

RNG2 depletion leads to conoid detachment from the apical pole of the parasite.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Detachment of the conoid in absence of RNG2 impairs parasite motility and invasion, additional phenotyping of the RNG2-mAID strain.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

RNG2 is functional only as intact, full-length protein.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Investigation of RNG2 subdomain functions.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Biochemical investigation of wild-type and processed RNG2.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

<i>Toxoplasma gondii</i> tachyzoite cell organization.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Reconstruction of the Fig 4E panel III.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Reconstruction of the Fig 4E panel II.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Reconstruction of the Fig 4E panel I.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Source Numerical Data of this study.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Additional information on antibodies and primers used in this study.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Additional information on the RNG2 variants expressed in UPRT locus.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Additional information on the cryo-electron tomography performed on RNG2-mAID strain.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Additional information on the processed forms and localization of the RNG2 protein.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Multiple epitopes-tagging across RNG2.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Additional information of the RNG2 purification from insect cells.
door: Romuald Haase (22339777)
Gepubliceerd in: (2025)

Spermidine Prevents Polarity Loss of Absorptive Enterocytes in Jejunum of Lipopolysaccharide-Challenged Mice via 4D-DIA Proteomics Analysis
door: Pengchao Zheng (22674589)
Gepubliceerd in: (2025)

Spermidine Prevents Polarity Loss of Absorptive Enterocytes in Jejunum of Lipopolysaccharide-Challenged Mice via 4D-DIA Proteomics Analysis
door: Pengchao Zheng (22674589)
Gepubliceerd in: (2025)

A. Mouse transcriptome non-negative matrix factorization component weights across all time points and conditions. Larger weight values indicate components with upregulated genes dominating. B. Gene set enrichment plot for selected features from each component in panel A. Components 1, 4, and 5 did not exhibit enrichment in these broad terms. C. Number of genes in enriched gene ontology terms related to immunity, signaling, and transcription for each time point and condition. D. Time course transcriptional profiles for mouse genes characteristic to component 3. E. PARFA-seq validation by quantitative PCR and gene expression changes during immortalized bone marrow-derived macrophage infection by <i>T. gondii</i> assessed by quantitative PCR.
door: Dominykas Murza (22676289)
Gepubliceerd in: (2025)

A. <i>T. gondii</i> gene group changes throughout the time course, expressed as log<sub>2</sub>(fold change) relative to pre-infection tachyzoites (t = 0). Grey area depicts the 95% confidence interval. n value refers to number of genes detected in each group. B. Gene set enrichment plot for most enriched terms from <i>T. gondii</i> transcriptome features at 15 min to 120 min. Five most significant terms are displayed for each database. C and D. Transcriptional profiles of top five upregulated and downregulated <i>T. gondii</i> transcription factors, respectively.
door: Dominykas Murza (22676289)
Gepubliceerd in: (2025)

A. Library preparation strategy: poly-A(+) RNAs are captured by complementarity to reverse transcription primer, then second strand is synthesized using random hexamers and handles for sequencing are attached through two rounds of PCR. B. Mapped read distribution by species and by biotype. C and D. Principal component analysis of mouse and <i>T. gondii</i> transcriptomes, respectively. E and F. Volcano plots of Live <i>Toxoplasma</i>-infected mouse and <i>T. gondii</i> time points, respectively. To calculate Log<sub>2</sub>(Fold change), every timepoint is compared to corresponding samples (either macrophage or <i>Toxoplasma</i>) before infection (t = 0 min). Most significant and most upregulated transcripts are labelled. Number of significantly (p ≤ 0.05) downregulated and upregulated genes for each time point is given in blue and red, respectively. In F, 24-hour time point, red dots mark ROP
door: Dominykas Murza (22676289)
Gepubliceerd in: (2025)

A. Experimental scheme of infection time course and downstream workflow; B. Optimization of tachyzoite concentration to achieve adequate infection rates, with a threshold set at 50% infection efficiency. Error bars represent ± 1 standard deviation. Corresponding MOI values are given for each condition; C. Infection time course micrograph merged from brightfield and green channels. <i>T. gondii</i> exhibits green fluorescence due to stable GFP expression in the strain used. Percentage at the top left indicates infection efficiency (± 1 standard deviation) for each time point.
door: Dominykas Murza (22676289)
Gepubliceerd in: (2025)

Summary of all significant gene enrichment terms for every condition (as combination of sample type and timepoint).
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Gepubliceerd in: (2025)

Oligonucleotides used in this study.
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Gepubliceerd in: (2025)

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