Strain-specific analysis of RVA RNA levels in extraintestinal tissues.
<p>Six-day-old germ-free pigs were orally inoculated with 1 × 10⁶ FFU of each rotavirus. At the indicated post-inoculation time points, animals were euthanized, and tissues (lungs, salivary glands, liver, blood, and spleen) were collected. <b>(A)</b> RT-qPCR RVA RNA detection frequ...
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2025
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| Итог: | <p>Six-day-old germ-free pigs were orally inoculated with 1 × 10⁶ FFU of each rotavirus. At the indicated post-inoculation time points, animals were euthanized, and tissues (lungs, salivary glands, liver, blood, and spleen) were collected. <b>(A)</b> RT-qPCR RVA RNA detection frequency across dpi 1–4; <b>(B)</b> Detection frequency at time points where significant differences were observed. The frequency of RVA detection across tissues from dpi 1–4 was assessed using Fisher’s exact test. Significant differences (*p < 0.05) are indicated. <b>(C)</b> ISH-based RVA RNA detection frequency across dpi 1–4; <b>(D)</b> Detection frequency at time points with significant differences. Statistical analysis was performed using Fisher’s exact test. Significant differences (*p < 0.05) are indicated.</p> |
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