Video 2_NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.avi
<p>Dendritic cells (DCs) are sentinels of the immune system and potent professional antigen-presenting cells with the ability to encounter antigens in the periphery, migrate to draining lymph nodes, and activate naive T cells. A major challenge in studying DC biology is the poor efficacy of en...
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| مؤلفون آخرون: | , , |
| منشور في: |
2025
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| _version_ | 1852017139734544384 |
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| author | Guerric P. B. Samson (11440729) |
| author2 | Nadine Anslinger (22152502) Daniel F. Legler (8745741) Daniela Finke (181180) |
| author2_role | author author author |
| author_facet | Guerric P. B. Samson (11440729) Nadine Anslinger (22152502) Daniel F. Legler (8745741) Daniela Finke (181180) |
| author_role | author |
| dc.creator.none.fl_str_mv | Guerric P. B. Samson (11440729) Nadine Anslinger (22152502) Daniel F. Legler (8745741) Daniela Finke (181180) |
| dc.date.none.fl_str_mv | 2025-09-01T05:21:37Z |
| dc.identifier.none.fl_str_mv | 10.3389/fimmu.2025.1658429.s002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/media/Video_2_NUPA10hd-immortalized_and_genetically_engineered_progenitors_allow_studying_dendritic_cell_immune_functions_avi/30021109 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Genetic Immunology HOX gene family cell migration dendritic cells cytokines immune responses |
| dc.title.none.fl_str_mv | Video 2_NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.avi |
| dc.type.none.fl_str_mv | Dataset Media info:eu-repo/semantics/publishedVersion dataset |
| description | <p>Dendritic cells (DCs) are sentinels of the immune system and potent professional antigen-presenting cells with the ability to encounter antigens in the periphery, migrate to draining lymph nodes, and activate naive T cells. A major challenge in studying DC biology is the poor efficacy of engineering them and generating stable genetically modified DC subsets for preclinical studies and transplantation purposes. Here, we extend studies on Hoxb8-immortalized progenitor cells, previously documented to differentiate into functional DCs, to another Hox-based strategy, namely, constitutive NUP98Hoxa10HD (NUPA10hd) expression in murine hematopoietic progenitor cells. We show that both NUPA10hd- and Hoxb8-immortalized progenitors give rise to functional DCs in vitro, which are capable of CCR7-driven migration and T-cell priming. In contrast to Hoxb8 progenitors, NUPA10hd progenitors show efficient and stable in vivo differentiation into plasmacytoid DCs (pDCs), conventional DC1s (cDC1s), and cDC2s. Finally, we demonstrate the efficacy of the NUPA10hd system in producing genetically modified DCs, allowing the monitoring of DC–T cell interactions and signaling events in migrating DCs. Collectively, NUPA10hd-immortalized progenitors represent a versatile and effective system for investigating immune functions of wild-type and genetically engineered DCs.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_2a34a7aa2a8a7a20831799d50a1bc422 |
| identifier_str_mv | 10.3389/fimmu.2025.1658429.s002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30021109 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Video 2_NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.aviGuerric P. B. Samson (11440729)Nadine Anslinger (22152502)Daniel F. Legler (8745741)Daniela Finke (181180)Genetic ImmunologyHOX gene familycell migrationdendritic cellscytokinesimmune responses<p>Dendritic cells (DCs) are sentinels of the immune system and potent professional antigen-presenting cells with the ability to encounter antigens in the periphery, migrate to draining lymph nodes, and activate naive T cells. A major challenge in studying DC biology is the poor efficacy of engineering them and generating stable genetically modified DC subsets for preclinical studies and transplantation purposes. Here, we extend studies on Hoxb8-immortalized progenitor cells, previously documented to differentiate into functional DCs, to another Hox-based strategy, namely, constitutive NUP98Hoxa10HD (NUPA10hd) expression in murine hematopoietic progenitor cells. We show that both NUPA10hd- and Hoxb8-immortalized progenitors give rise to functional DCs in vitro, which are capable of CCR7-driven migration and T-cell priming. In contrast to Hoxb8 progenitors, NUPA10hd progenitors show efficient and stable in vivo differentiation into plasmacytoid DCs (pDCs), conventional DC1s (cDC1s), and cDC2s. Finally, we demonstrate the efficacy of the NUPA10hd system in producing genetically modified DCs, allowing the monitoring of DC–T cell interactions and signaling events in migrating DCs. Collectively, NUPA10hd-immortalized progenitors represent a versatile and effective system for investigating immune functions of wild-type and genetically engineered DCs.</p>2025-09-01T05:21:37ZDatasetMediainfo:eu-repo/semantics/publishedVersiondataset10.3389/fimmu.2025.1658429.s002https://figshare.com/articles/media/Video_2_NUPA10hd-immortalized_and_genetically_engineered_progenitors_allow_studying_dendritic_cell_immune_functions_avi/30021109CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/300211092025-09-01T05:21:37Z |
| spellingShingle | Video 2_NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.avi Guerric P. B. Samson (11440729) Genetic Immunology HOX gene family cell migration dendritic cells cytokines immune responses |
| status_str | publishedVersion |
| title | Video 2_NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.avi |
| title_full | Video 2_NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.avi |
| title_fullStr | Video 2_NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.avi |
| title_full_unstemmed | Video 2_NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.avi |
| title_short | Video 2_NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.avi |
| title_sort | Video 2_NUPA10hd-immortalized and genetically engineered progenitors allow studying dendritic cell immune functions.avi |
| topic | Genetic Immunology HOX gene family cell migration dendritic cells cytokines immune responses |