Figure 4 from Extracellular Vesicle Secretion by Leukemia Cells <i>In Vivo</i> Promotes CLL Progression by Hampering Antitumor T-cell Responses
<p>LME-sEVs impact CD8<sup>+</sup> T-cell transcriptome, proteome, and metabolome. <b>A,</b> Volcano plot showing DEG identified by RNA-seq from CD8<sup>+</sup> T cells treated for 48 hours with LME- (<i>n</i> = 4) or HCME-sEVs (<i>n</i&...
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2025
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| Resumo: | <p>LME-sEVs impact CD8<sup>+</sup> T-cell transcriptome, proteome, and metabolome. <b>A,</b> Volcano plot showing DEG identified by RNA-seq from CD8<sup>+</sup> T cells treated for 48 hours with LME- (<i>n</i> = 4) or HCME-sEVs (<i>n</i> = 3) with FDR <0.05 and log<sub>2</sub>FC >1. <b>B</b> and <b>C,</b> Hierarchical clustering of all DEG (<b>B</b>) and of selected genes from relevant cell functions (<b>C</b>). <b>D,</b> Volcano plot showing differentially expressed proteins (DEP) identified by mass spectrometry from CD8<sup>+</sup> T cells treated for 96 hours with LME- (<i>n</i> = 3) and HCME-sEVs (<i>n</i> = 3) with FDR <0.05 and log<sub>2</sub>FC >1. <b>E</b> and <b>F,</b> GzmB mRNA expression and GzmB and perforin levels in CD8<sup>+</sup> T cells treated for 48 hours with HCME- or LME-sEVs. <b>G</b> and <b>H,</b> Ontology analysis of enriched (<b>G</b>) or diminished (<b>H</b>) DEP in CD8<sup>+</sup> T cells treated with LME- or HCME-sEVs (from <b>D</b>). <b>I,</b> Levels of glucose measured by mass spectrometry in culture medium in CD8<sup>+</sup> T-cell treated with LME- or HCME-sEVs for 96 hours. Negative value represents consumption. <b>J,</b> Immunoblot analysis of glycolysis-related proteins from CD8<sup>+</sup> T cells treated for 96 hours with LME- or HCME-sEVs. <b>K,</b> Levels of ADP and ATP generated from <sup>13</sup>C-glucose measured by mass spectrometry in CD8<sup>+</sup> T cells treated with LME- (<i>n</i> = 5) or HCME-sEVs (<i>n</i> = 6) for 96 hours. <b>L,</b> Oxygen consumption measured by SeaHorse assay from CD8<sup>+</sup> T cells treated with LME- or HCME-sEVs for 96h. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ****, <i>P</i> < 0.0001 (unpaired Student <i>t</i> test). Data are mean and SEM.</p> |
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