Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models

Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models

<p>ART558 treatment leads to increased residual IR-induced DNA damage foci. <b>A,</b> γH2AX and 53BP1 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed at the indicated times points after IR. <b>B,</b> Representative images from irradiated...

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Bibliografski detalji
Glavni autor: Gonzalo Rodriguez-Berriguete (15056057) (author)
Daljnji autori: Marco Ranzani (15056060) (author), Remko Prevo (15056063) (author), Rathi Puliyadi (15056066) (author), Nicole Machado (15056069) (author), Hannah R. Bolland (15056072) (author), Val Millar (15056075) (author), Daniel Ebner (15056078) (author), Marie Boursier (15056081) (author), Aurora Cerutti (15056084) (author), Alessandro Cicconi (15056087) (author), Alessandro Galbiati (15056090) (author), Diego Grande (15056093) (author), Vera Grinkevich (15056096) (author), Jayesh B. Majithiya (15056099) (author), Desiree Piscitello (15056102) (author), Eeson Rajendra (15056105) (author), Martin L. Stockley (15056108) (author), Simon J. Boulton (15056111) (author), Ester M. Hammond (15056114) (author), Robert A. Heald (15056117) (author), Graeme C.M. Smith (15056120) (author), Helen M.R. Robinson (15056123) (author), Geoff S. Higgins (15056126) (author)
Izdano: 2025
Teme:
Cancer
Molecular and Cellular Biology
Therapeutic Research and Development
DNA Damage and Repair
DNA repair
Radiation Oncology
Molecular targets of radiation response
Small Molecule Agents
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Opis
Sažetak:<p>ART558 treatment leads to increased residual IR-induced DNA damage foci. <b>A,</b> γH2AX and 53BP1 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed at the indicated times points after IR. <b>B,</b> Representative images from irradiated cells from the experiment described in <b>A</b>, at 16 hours after IR. <b>C,</b> Micronuclei in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed 48 hours after IR. Data points indicate the mean ± SD from triplicate wells and graphs are representative from three separate experiments. <b>D,</b> Representative images from irradiated cells from the experiment described in <b>C</b>. Micronuclei are indicated with arrow heads. dsDNA, double-stranded DNA. <b>E,</b> RAD51 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed 6 hours after IR. <b>F,</b> Representative images from irradiated cells from the experiment described in <b>E</b>. Lines and error bars in <b>A</b> and <b>E</b> correspond to average ± SD representative from three independent experiments, and statistical significance was calculated using nonparametric one-way ANOVA (Kruskal–Wallis) with Dunn correction for multiple comparisons (*, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001).</p>

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