Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models
<p>ART558 treatment leads to increased residual IR-induced DNA damage foci. <b>A,</b> γH2AX and 53BP1 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed at the indicated times points after IR. <b>B,</b> Representative images from irradiated...
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| _version_ | 1849927633239801856 |
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| author | Gonzalo Rodriguez-Berriguete (15056057) |
| author2 | Marco Ranzani (15056060) Remko Prevo (15056063) Rathi Puliyadi (15056066) Nicole Machado (15056069) Hannah R. Bolland (15056072) Val Millar (15056075) Daniel Ebner (15056078) Marie Boursier (15056081) Aurora Cerutti (15056084) Alessandro Cicconi (15056087) Alessandro Galbiati (15056090) Diego Grande (15056093) Vera Grinkevich (15056096) Jayesh B. Majithiya (15056099) Desiree Piscitello (15056102) Eeson Rajendra (15056105) Martin L. Stockley (15056108) Simon J. Boulton (15056111) Ester M. Hammond (15056114) Robert A. Heald (15056117) Graeme C.M. Smith (15056120) Helen M.R. Robinson (15056123) Geoff S. Higgins (15056126) |
| author2_role | author author author author author author author author author author author author author author author author author author author author author author author |
| author_facet | Gonzalo Rodriguez-Berriguete (15056057) Marco Ranzani (15056060) Remko Prevo (15056063) Rathi Puliyadi (15056066) Nicole Machado (15056069) Hannah R. Bolland (15056072) Val Millar (15056075) Daniel Ebner (15056078) Marie Boursier (15056081) Aurora Cerutti (15056084) Alessandro Cicconi (15056087) Alessandro Galbiati (15056090) Diego Grande (15056093) Vera Grinkevich (15056096) Jayesh B. Majithiya (15056099) Desiree Piscitello (15056102) Eeson Rajendra (15056105) Martin L. Stockley (15056108) Simon J. Boulton (15056111) Ester M. Hammond (15056114) Robert A. Heald (15056117) Graeme C.M. Smith (15056120) Helen M.R. Robinson (15056123) Geoff S. Higgins (15056126) |
| author_role | author |
| dc.creator.none.fl_str_mv | Gonzalo Rodriguez-Berriguete (15056057) Marco Ranzani (15056060) Remko Prevo (15056063) Rathi Puliyadi (15056066) Nicole Machado (15056069) Hannah R. Bolland (15056072) Val Millar (15056075) Daniel Ebner (15056078) Marie Boursier (15056081) Aurora Cerutti (15056084) Alessandro Cicconi (15056087) Alessandro Galbiati (15056090) Diego Grande (15056093) Vera Grinkevich (15056096) Jayesh B. Majithiya (15056099) Desiree Piscitello (15056102) Eeson Rajendra (15056105) Martin L. Stockley (15056108) Simon J. Boulton (15056111) Ester M. Hammond (15056114) Robert A. Heald (15056117) Graeme C.M. Smith (15056120) Helen M.R. Robinson (15056123) Geoff S. Higgins (15056126) |
| dc.date.none.fl_str_mv | 2025-11-25T13:08:00Z |
| dc.identifier.none.fl_str_mv | 10.1158/1078-0432.30707644 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Figure_2_from_Small-Molecule_Pol_Inhibitors_Provide_Safe_and_Effective_Tumor_Radiosensitization_in_Preclinical_Models/30707644 |
| dc.rights.none.fl_str_mv | CC BY info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cancer Molecular and Cellular Biology Therapeutic Research and Development DNA Damage and Repair DNA repair Radiation Oncology Molecular targets of radiation response Small Molecule Agents |
| dc.title.none.fl_str_mv | Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>ART558 treatment leads to increased residual IR-induced DNA damage foci. <b>A,</b> γH2AX and 53BP1 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed at the indicated times points after IR. <b>B,</b> Representative images from irradiated cells from the experiment described in <b>A</b>, at 16 hours after IR. <b>C,</b> Micronuclei in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed 48 hours after IR. Data points indicate the mean ± SD from triplicate wells and graphs are representative from three separate experiments. <b>D,</b> Representative images from irradiated cells from the experiment described in <b>C</b>. Micronuclei are indicated with arrow heads. dsDNA, double-stranded DNA. <b>E,</b> RAD51 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed 6 hours after IR. <b>F,</b> Representative images from irradiated cells from the experiment described in <b>E</b>. Lines and error bars in <b>A</b> and <b>E</b> correspond to average ± SD representative from three independent experiments, and statistical significance was calculated using nonparametric one-way ANOVA (Kruskal–Wallis) with Dunn correction for multiple comparisons (*, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001).</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_2ca34954a588425653a6628a7c61c3c0 |
| identifier_str_mv | 10.1158/1078-0432.30707644 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30707644 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY |
| spelling | Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical ModelsGonzalo Rodriguez-Berriguete (15056057)Marco Ranzani (15056060)Remko Prevo (15056063)Rathi Puliyadi (15056066)Nicole Machado (15056069)Hannah R. Bolland (15056072)Val Millar (15056075)Daniel Ebner (15056078)Marie Boursier (15056081)Aurora Cerutti (15056084)Alessandro Cicconi (15056087)Alessandro Galbiati (15056090)Diego Grande (15056093)Vera Grinkevich (15056096)Jayesh B. Majithiya (15056099)Desiree Piscitello (15056102)Eeson Rajendra (15056105)Martin L. Stockley (15056108)Simon J. Boulton (15056111)Ester M. Hammond (15056114)Robert A. Heald (15056117)Graeme C.M. Smith (15056120)Helen M.R. Robinson (15056123)Geoff S. Higgins (15056126)CancerMolecular and Cellular BiologyTherapeutic Research and DevelopmentDNA Damage and RepairDNA repairRadiation OncologyMolecular targets of radiation responseSmall Molecule Agents<p>ART558 treatment leads to increased residual IR-induced DNA damage foci. <b>A,</b> γH2AX and 53BP1 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed at the indicated times points after IR. <b>B,</b> Representative images from irradiated cells from the experiment described in <b>A</b>, at 16 hours after IR. <b>C,</b> Micronuclei in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed 48 hours after IR. Data points indicate the mean ± SD from triplicate wells and graphs are representative from three separate experiments. <b>D,</b> Representative images from irradiated cells from the experiment described in <b>C</b>. Micronuclei are indicated with arrow heads. dsDNA, double-stranded DNA. <b>E,</b> RAD51 foci in HCT116 and H460 cells treated with 3 μmol/L ART558 and 4 Gy IR, assessed 6 hours after IR. <b>F,</b> Representative images from irradiated cells from the experiment described in <b>E</b>. Lines and error bars in <b>A</b> and <b>E</b> correspond to average ± SD representative from three independent experiments, and statistical significance was calculated using nonparametric one-way ANOVA (Kruskal–Wallis) with Dunn correction for multiple comparisons (*, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001).</p>2025-11-25T13:08:00ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1158/1078-0432.30707644https://figshare.com/articles/figure/Figure_2_from_Small-Molecule_Pol_Inhibitors_Provide_Safe_and_Effective_Tumor_Radiosensitization_in_Preclinical_Models/30707644CC BYinfo:eu-repo/semantics/openAccessoai:figshare.com:article/307076442025-11-25T13:08:00Z |
| spellingShingle | Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models Gonzalo Rodriguez-Berriguete (15056057) Cancer Molecular and Cellular Biology Therapeutic Research and Development DNA Damage and Repair DNA repair Radiation Oncology Molecular targets of radiation response Small Molecule Agents |
| status_str | publishedVersion |
| title | Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models |
| title_full | Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models |
| title_fullStr | Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models |
| title_full_unstemmed | Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models |
| title_short | Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models |
| title_sort | Figure 2 from Small-Molecule Polθ Inhibitors Provide Safe and Effective Tumor Radiosensitization in Preclinical Models |
| topic | Cancer Molecular and Cellular Biology Therapeutic Research and Development DNA Damage and Repair DNA repair Radiation Oncology Molecular targets of radiation response Small Molecule Agents |