Serves as the data source for Figs 1 and 2.
<div><p>Antibiotic resistance is a critical global public health issue. The gut microbiome acts as a reservoir for numerous antibiotic resistance genes (ARGs), which influence both existing and future microbial populations within a community or ecosystem. However, the differences in ARG...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , |
| منشور في: |
2025
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| الموضوعات: | |
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إضافة وسم
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| _version_ | 1852023181249871872 |
|---|---|
| author | Shuai Huang (60512) |
| author2 | Minghui Xing (6239537) Haifeng Wang (188956) |
| author2_role | author author |
| author_facet | Shuai Huang (60512) Minghui Xing (6239537) Haifeng Wang (188956) |
| author_role | author |
| dc.creator.none.fl_str_mv | Shuai Huang (60512) Minghui Xing (6239537) Haifeng Wang (188956) |
| dc.date.none.fl_str_mv | 2025-01-29T18:32:20Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0317827.s002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Serves_as_the_data_source_for_Figs_1_and_2_/28305537 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Microbiology Cell Biology Genetics Ecology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified gut microbiome acts composting substantially transforms collected eight samples 25 genes downregulated principal coordinate analysis div >< p microbial community structure antibiotic resistance genes composted pig manure fresh pig manure china </ p antibiotic resistance pig manure comparative analysis p </ fresh manure blaoxy </ blaoch </ 01 </ two types potential role pcoa ). kaifeng city increased abundance decreased abundance arg profiles arg profile arg expression 14 upregulated |
| dc.title.none.fl_str_mv | Serves as the data source for Figs 1 and 2. |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <div><p>Antibiotic resistance is a critical global public health issue. The gut microbiome acts as a reservoir for numerous antibiotic resistance genes (ARGs), which influence both existing and future microbial populations within a community or ecosystem. However, the differences in ARG expression between fresh and composted feces remain poorly understood. In this study, we collected eight samples from a farm in Kaifeng City, China, comprising both fresh and composted pig manure. Using a high-throughput quantitative PCR array, we analyzed differences in ARG expression between these two types of manure. Our findings revealed significant differences in ARG profiles, as demonstrated by principal coordinate analysis (PCoA). Further analysis identified 39 ARGs (log2FC > 1, <i>p</i> < 0.05) in composted pig manure, with 25 genes downregulated and 14 upregulated. Notably, <i>tetB-01</i>, <i>blaOCH</i>, and <i>blaOXY</i> were the most abundant in composted pig manure compared to fresh manure. Additionally, 16S rRNA species profiling revealed that the composting process significantly altered the microbial community structure, with an increased abundance of Firmicutes and a decreased abundance of Bacteroidetes in composted pig manure. In summary, composting substantially transforms both the microbial community structure and the ARG profile in pig manure, underscoring its potential role in modulating the dynamics of ARGs in agricultural environments.</p></div> |
| eu_rights_str_mv | openAccess |
| id | Manara_2db5cbc2da955b7aec08dbd049fe15e0 |
| identifier_str_mv | 10.1371/journal.pone.0317827.s002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/28305537 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Serves as the data source for Figs 1 and 2.Shuai Huang (60512)Minghui Xing (6239537)Haifeng Wang (188956)MicrobiologyCell BiologyGeneticsEcologyEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedgut microbiome actscomposting substantially transformscollected eight samples25 genes downregulatedprincipal coordinate analysisdiv >< pmicrobial community structureantibiotic resistance genescomposted pig manurefresh pig manurechina </ pantibiotic resistancepig manurecomparative analysisp </fresh manureblaoxy </blaoch </01 </two typespotential rolepcoa ).kaifeng cityincreased abundancedecreased abundancearg profilesarg profilearg expression14 upregulated<div><p>Antibiotic resistance is a critical global public health issue. The gut microbiome acts as a reservoir for numerous antibiotic resistance genes (ARGs), which influence both existing and future microbial populations within a community or ecosystem. However, the differences in ARG expression between fresh and composted feces remain poorly understood. In this study, we collected eight samples from a farm in Kaifeng City, China, comprising both fresh and composted pig manure. Using a high-throughput quantitative PCR array, we analyzed differences in ARG expression between these two types of manure. Our findings revealed significant differences in ARG profiles, as demonstrated by principal coordinate analysis (PCoA). Further analysis identified 39 ARGs (log2FC > 1, <i>p</i> < 0.05) in composted pig manure, with 25 genes downregulated and 14 upregulated. Notably, <i>tetB-01</i>, <i>blaOCH</i>, and <i>blaOXY</i> were the most abundant in composted pig manure compared to fresh manure. Additionally, 16S rRNA species profiling revealed that the composting process significantly altered the microbial community structure, with an increased abundance of Firmicutes and a decreased abundance of Bacteroidetes in composted pig manure. In summary, composting substantially transforms both the microbial community structure and the ARG profile in pig manure, underscoring its potential role in modulating the dynamics of ARGs in agricultural environments.</p></div>2025-01-29T18:32:20ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.1371/journal.pone.0317827.s002https://figshare.com/articles/dataset/Serves_as_the_data_source_for_Figs_1_and_2_/28305537CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/283055372025-01-29T18:32:20Z |
| spellingShingle | Serves as the data source for Figs 1 and 2. Shuai Huang (60512) Microbiology Cell Biology Genetics Ecology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified gut microbiome acts composting substantially transforms collected eight samples 25 genes downregulated principal coordinate analysis div >< p microbial community structure antibiotic resistance genes composted pig manure fresh pig manure china </ p antibiotic resistance pig manure comparative analysis p </ fresh manure blaoxy </ blaoch </ 01 </ two types potential role pcoa ). kaifeng city increased abundance decreased abundance arg profiles arg profile arg expression 14 upregulated |
| status_str | publishedVersion |
| title | Serves as the data source for Figs 1 and 2. |
| title_full | Serves as the data source for Figs 1 and 2. |
| title_fullStr | Serves as the data source for Figs 1 and 2. |
| title_full_unstemmed | Serves as the data source for Figs 1 and 2. |
| title_short | Serves as the data source for Figs 1 and 2. |
| title_sort | Serves as the data source for Figs 1 and 2. |
| topic | Microbiology Cell Biology Genetics Ecology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified gut microbiome acts composting substantially transforms collected eight samples 25 genes downregulated principal coordinate analysis div >< p microbial community structure antibiotic resistance genes composted pig manure fresh pig manure china </ p antibiotic resistance pig manure comparative analysis p </ fresh manure blaoxy </ blaoch </ 01 </ two types potential role pcoa ). kaifeng city increased abundance decreased abundance arg profiles arg profile arg expression 14 upregulated |