Schematic overview of cyanogenesis in <i>Chamberlinius hualienensis</i> and cyanohydrin biosynthesis in millipedes, insects, and plants.

Schematic overview of cyanogenesis in <i>Chamberlinius hualienensis</i> and cyanohydrin biosynthesis in millipedes, insects, and plants.

<p>(A) <i>C. hualienensis</i> and a schematic view of cyanogenesis. Paranota of the millipede house defensive glands and consist of reservoir and reaction chamber. (<i>R</i>)-Mandelonitrile (MAN) stored in the reservoir is admitted to the reaction chamber, converted by...

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Bibliografische gegevens
Hoofdauteur:	Takuya Yamaguchi (4543105) (author)
Andere auteurs:	Yasuhisa Asano (727606) (author)
Gepubliceerd in:	2025
Onderwerpen:	Biochemistry Evolutionary Biology Ecology Plant Biology Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified subsequently converting aldoxime natural defensive chemicals indicating sequential duplication catalyse aldoxime dehydration div >< p cyanogenic millipedes evolved chamberlinius hualienensis </ hualienensis </ r </ typically lineage related enzymes plant lineages plant kingdoms paralogous genes millipedes demonstrates mechanisms underlying hydroxynitrile lyase hydrogen cyanide giving rise enzymes independently dependent monooxygenase deeper understanding cytochrome p450 complete set coding genes biological characterisation >)- mandelonitrile
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Distribution of cyanogenesis-related enzymes in millipedes.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Assembly and annotation statistics of <i>Chamberlinius hualienensis</i> genome.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Body weight and accumulation of (<i>R</i>)-mandelonitrile in <i>Chamberlinius hualienensis.</i>
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Characteristics of flavin-dependent monooxygenases (FMOs) from <i>Chamberlinius hualienensis.</i>
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Identification of phenylacetaldoxime (PAOx)-producing flavin dependent monooxygenase from <i>Chamberlinius hualienensis.</i>
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Overview of the processing pipeline used for the assembly of the <i>Chamberlinius hualienensis genome</i> (see materials and methods for detail).
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Amino acid sequence alignment (A) and amino acid sequence identity (B) of CHUA_008368 (CYP4GL4) from <i>Chamberlinius hualienensis</i>, Hho_020879 from <i>Helicorthomorpha holstii.</i>
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Amino acid sequence alignment (A) and amino acid sequence identity (B) of CHUA_003298 (ChuaMOxS) from <i>Chamberlinius hualienensis</i>, Hho_019011 from <i>Helicorthomorpha holstii</i>, and Nno_033811 from <i>Niponia nodulosa.</i>
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

<i>C. hualienensis</i> genome annotation.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Amino acid sequence alignment (A) and and amino acid sequence identity (B) of CHUA_014123 (CYP30008A2) from <i>Chamberlinius hualienensis</i> and its orthorogous proteins, Hho_011653 from <i>Helicorthomorpha holstii</i> and Nno_033394 from <i>Niponia nodulosa.</i>
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Identification of (<i>R</i>)-mandelonitrile biosynthetic enzymes from <i>C. hualienensis.</i>
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

TE contents of millipede and centipede.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

GenomeScope plot of the 21-mer content within the <i>C. hualienensis</i> genome.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Detection of aromatic amino acids-derived aldoximes after the culture of <i>Escherichia coli</i> harboring millipede aldoxime synthase (MOxS, CHUA_003298).
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Amino acid sequence alignment (A) and sequence identity matrix (B) of <i>Chamberliniensis hualienensis</i> flavin-dependent monooxygenases.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Correlations of genome sizes and TE contents among millipedes and centipedes.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Three dimensional structures of hydroxynitrile lyase (HNL) from millipede, plant, and microorganism.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Phylogenetic tree of millipede HNLs, their paralogous proteins, and lipocalins from animals (arthropoda and chordata), plants, and bacteria.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i>
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Gene cluster and characterisation of <i>ChuaHNL</i> and its paralogues.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

TMM normalized read counts of (<i>R</i>)-mandelonitrile biosynthetic genes.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Heterologous production of ChuaHNL and its paralogous proteins in <i>Pichia pastoris.</i>
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Substrate saturation curves for CHUA_005138 (ChuaHNL), CHUA_005137, and CHUA_005136 with (<i>R,S</i>)-mandelonitrile (mandelonitrile cleavage reaction) (A) or benzaldehyde ((<i>R</i>)-mandelonitrile synthetic reaction) (B).
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

(<i>R</i>)-mandelonitrile synthetic reaction catalyzed by CHUA_005138 (ChuaHNL), CHUA_005137, and CHUA_005136.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Purification of N-terminal His-tagged CHUA_005136 from 1.5 L of <i>Pichia pastoris</i> culture supernatant.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Purification of N-terminal His-tagged CHUA_005137 from 1.5 L of <i>Pichia pastoris</i> culture supernatant.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Purification of N-terminal His-tagged ChuaHNL (CHUA_005138) from 1.5 L of <i>Pichia pastoris</i> culture supernatant.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Characterization of CYP4GL4 (CHUA_008368) and CYP30008A2 (CHUA_014123) catalyzing dehydration of (<i>E/Z</i>)-phenylacetaldoxime (PAOx) into phenylacetonitrile.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Formation of phenylacetonitrile (PAN) from (<i>E/Z</i>)-phenylacetaldoxime by yeast harboring CHUA_008368 (CYP4GL4) and CHUA_014123 (CYP30008A2).
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Numerical data underlying Figs 3, 4E, S4, S8, S10, S12, S13,
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

TMM normalized read counts of flavin-dependent monooxygenases (FMOs).
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

RNA-seq for gene expression analysis.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

RNA-seq for gene annotation.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Shotgun sequencing summary statistics (PacBio).
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Shotgun sequencing summary statistics (Illumina).
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Flavin-dependent monooxygenases in myriapods and other species.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Substrate specificity of CYP4GL4 (CHUA_008368) and CYP30008A2 (CHUA_014123) toward aromatic aldoximes.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Phylogenetic tree of cytochrome P450s.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Amino acid sequence alignment of ChuaHNL and its paralogous proteins.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

Amino acid sequence identity matrix of ChuaHNL and its paralogous proteins.
door: Takuya Yamaguchi (4543105)
Gepubliceerd in: (2025)

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