Data Sheet 1_Long-chain unsaturated free fatty acids reduce the host cell invasion of Listeria monocytogenes outbreak strains.pdf
<p>The Gram-positive bacterium Listeria monocytogenes is a highly adaptable pathogen capable of causing severe foodborne infections, particularly in vulnerable populations. During infection, L. monocytogenes uses a variety of virulence factors to invade and multiply within host cells. The tran...
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2025
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| _version_ | 1852022401461649408 |
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| author | Caroline Borreby (20799791) |
| author2 | Thea Amalie Hvidtfeldt (20799794) Magnus Ganer Jespersen (12491560) Patricia T. dos Santos (12491566) Sofie Dam Houborg (20799797) Eva Maria Sternkopf Lillebæk (9723494) Michael Kemp (6531443) Birgitte H. Kallipolitis (7331498) |
| author2_role | author author author author author author author |
| author_facet | Caroline Borreby (20799791) Thea Amalie Hvidtfeldt (20799794) Magnus Ganer Jespersen (12491560) Patricia T. dos Santos (12491566) Sofie Dam Houborg (20799797) Eva Maria Sternkopf Lillebæk (9723494) Michael Kemp (6531443) Birgitte H. Kallipolitis (7331498) |
| author_role | author |
| dc.creator.none.fl_str_mv | Caroline Borreby (20799791) Thea Amalie Hvidtfeldt (20799794) Magnus Ganer Jespersen (12491560) Patricia T. dos Santos (12491566) Sofie Dam Houborg (20799797) Eva Maria Sternkopf Lillebæk (9723494) Michael Kemp (6531443) Birgitte H. Kallipolitis (7331498) |
| dc.date.none.fl_str_mv | 2025-02-28T06:51:54Z |
| dc.identifier.none.fl_str_mv | 10.3389/fcimb.2025.1542165.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Data_Sheet_1_Long-chain_unsaturated_free_fatty_acids_reduce_the_host_cell_invasion_of_Listeria_monocytogenes_outbreak_strains_pdf/28511327 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Clinical Microbiology Listeria monocytogenes PrfA antimicrobial anti-virulence free fatty acids |
| dc.title.none.fl_str_mv | Data Sheet 1_Long-chain unsaturated free fatty acids reduce the host cell invasion of Listeria monocytogenes outbreak strains.pdf |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <p>The Gram-positive bacterium Listeria monocytogenes is a highly adaptable pathogen capable of causing severe foodborne infections, particularly in vulnerable populations. During infection, L. monocytogenes uses a variety of virulence factors to invade and multiply within host cells. The transcriptional regulator PrfA controls the expression of these virulence factors and is essential for the intracellular lifestyle of L. monocytogenes. Long-chain unsaturated free fatty acids (FFAs) have long been recognized for their antimicrobial activity and were recently shown to inhibit PrfA-dependent virulence gene expression in L. monocytogenes. To date, the antimicrobial and anti-virulent activities of FFAs have been primarily studied in laboratory strains. However, to fully evaluate their potential as anti-infective agents, it is essential to assess the effects of long-chain FFAs on clinically relevant isolates, including outbreak strains associated with high-fat food products. Here, we demonstrate that five different clinically relevant L. monocytogenes isolates are sensitive to the antimicrobial activity of long-chain unsaturated FFAs. Furthermore, at subinhibitory concentrations, these FFAs inhibit PrfA-regulated expression of virulence factors across all tested strains and reduce their invasive potential in non-phagocytic cells. These findings underscore the potential of long-chain unsaturated FFAs in developing new preventive strategies against L. monocytogenes strains associated with severe foodborne infections.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_312cba34dcafe755fb4bf7b6bd963b99 |
| identifier_str_mv | 10.3389/fcimb.2025.1542165.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/28511327 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Data Sheet 1_Long-chain unsaturated free fatty acids reduce the host cell invasion of Listeria monocytogenes outbreak strains.pdfCaroline Borreby (20799791)Thea Amalie Hvidtfeldt (20799794)Magnus Ganer Jespersen (12491560)Patricia T. dos Santos (12491566)Sofie Dam Houborg (20799797)Eva Maria Sternkopf Lillebæk (9723494)Michael Kemp (6531443)Birgitte H. Kallipolitis (7331498)Clinical MicrobiologyListeria monocytogenesPrfAantimicrobialanti-virulencefree fatty acids<p>The Gram-positive bacterium Listeria monocytogenes is a highly adaptable pathogen capable of causing severe foodborne infections, particularly in vulnerable populations. During infection, L. monocytogenes uses a variety of virulence factors to invade and multiply within host cells. The transcriptional regulator PrfA controls the expression of these virulence factors and is essential for the intracellular lifestyle of L. monocytogenes. Long-chain unsaturated free fatty acids (FFAs) have long been recognized for their antimicrobial activity and were recently shown to inhibit PrfA-dependent virulence gene expression in L. monocytogenes. To date, the antimicrobial and anti-virulent activities of FFAs have been primarily studied in laboratory strains. However, to fully evaluate their potential as anti-infective agents, it is essential to assess the effects of long-chain FFAs on clinically relevant isolates, including outbreak strains associated with high-fat food products. Here, we demonstrate that five different clinically relevant L. monocytogenes isolates are sensitive to the antimicrobial activity of long-chain unsaturated FFAs. Furthermore, at subinhibitory concentrations, these FFAs inhibit PrfA-regulated expression of virulence factors across all tested strains and reduce their invasive potential in non-phagocytic cells. These findings underscore the potential of long-chain unsaturated FFAs in developing new preventive strategies against L. monocytogenes strains associated with severe foodborne infections.</p>2025-02-28T06:51:54ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fcimb.2025.1542165.s001https://figshare.com/articles/dataset/Data_Sheet_1_Long-chain_unsaturated_free_fatty_acids_reduce_the_host_cell_invasion_of_Listeria_monocytogenes_outbreak_strains_pdf/28511327CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/285113272025-02-28T06:51:54Z |
| spellingShingle | Data Sheet 1_Long-chain unsaturated free fatty acids reduce the host cell invasion of Listeria monocytogenes outbreak strains.pdf Caroline Borreby (20799791) Clinical Microbiology Listeria monocytogenes PrfA antimicrobial anti-virulence free fatty acids |
| status_str | publishedVersion |
| title | Data Sheet 1_Long-chain unsaturated free fatty acids reduce the host cell invasion of Listeria monocytogenes outbreak strains.pdf |
| title_full | Data Sheet 1_Long-chain unsaturated free fatty acids reduce the host cell invasion of Listeria monocytogenes outbreak strains.pdf |
| title_fullStr | Data Sheet 1_Long-chain unsaturated free fatty acids reduce the host cell invasion of Listeria monocytogenes outbreak strains.pdf |
| title_full_unstemmed | Data Sheet 1_Long-chain unsaturated free fatty acids reduce the host cell invasion of Listeria monocytogenes outbreak strains.pdf |
| title_short | Data Sheet 1_Long-chain unsaturated free fatty acids reduce the host cell invasion of Listeria monocytogenes outbreak strains.pdf |
| title_sort | Data Sheet 1_Long-chain unsaturated free fatty acids reduce the host cell invasion of Listeria monocytogenes outbreak strains.pdf |
| topic | Clinical Microbiology Listeria monocytogenes PrfA antimicrobial anti-virulence free fatty acids |