Additional information on the cryo-electron tomography performed on RNG2-mAID strain.

Additional information on the cryo-electron tomography performed on RNG2-mAID strain.

<p><b>(A)</b> Tomogram slices presented in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.g004" target="_blank">Fig 4</a> without any overlay <b>(B)</b> Tomogram slices with and without over...

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Մատենագիտական մանրամասներ
Հիմնական հեղինակ: Romuald Haase (22339777) (author)
Այլ հեղինակներ: Bingjian Ren (22676577) (author), Albert Tell i Puig (22676580) (author), Alessandro Bonavoglia (19415718) (author), Jean-Baptiste Marq (375239) (author), Rémy Visentin (22676583) (author), Nicolas Dos Santos Pacheco (22676586) (author), Bohumil Maco (383443) (author), Ricardo Mondragón-Flores (10737057) (author), Oscar Vadas (2416228) (author), Dominique Soldati-Favre (79026) (author)
Հրապարակվել է: 2025
Խորագրեր:
Biophysics
Biochemistry
Microbiology
Cell Biology
Physiology
Infectious Diseases
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Physical Sciences not elsewhere classified
toxoplasma gondii </
remains apically anchored
heterologous expression systems
div >< p
apical polar ring
biochemical analysis revealed
proper conoid anchorage
ensures conoid functionality
less parasites revealed
host cell invasion
enable parasite motility
controlling parasite motility
although rng2 depletion
intact conoid organelle
organelle traverses
intact protein
conditional depletion
biochemical properties
detached conoid
gliding motility
unstable protein
undergoes extrusion
two structures
strong candidate
striking detachment
still unclear
rng2 tethers
rng2 led
rng2 emerges
rhoptries follow
resilient bridge
protein localized
plasma membrane
pivotal protein
likely facilitate
large coiled
immunoelectron microscopy
functional activity
enabling rng2
electron tomography
dependent extrusion
critical role
concatenated assemblies
comprehensive mutagenesis
coil domains
calcium waves
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Նկարագրություն
Ամփոփում:<p><b>(A)</b> Tomogram slices presented in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003506#pbio.3003506.g004" target="_blank">Fig 4</a> without any overlay <b>(B)</b> Tomogram slices with and without overlay of untreated RNG2-mAID showing the correct positioning of the elements of the apical complex. <b>(C)</b> IFA images showing the localization of the Nd6 protein in presence or absence of RNG2. <b>(D)</b> U-ExM images showing the localization of the Nd6 protein in with the conoid attached or detached from the apical pole of the parasite.</p> <p>(TIFF)</p>

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